Summary of Study ST002187

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR001394. The data can be accessed directly via it's Project DOI: 10.21228/M8J12Z This work is supported by NIH grant, U2C- DK119886.


This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

Perform statistical analysis  |  Show all samples  |  Show named metabolites  |  Download named metabolite data  
Download mwTab file (text)   |  Download mwTab file(JSON)   |  Download data files (Contains raw data)
Study IDST002187
Study TitleInterplay of CodY and CcpA in regulating central metabolism and biofilm formation in S. aureus
Study TypeResearch
Study SummaryStaphylococcus aureus is a medically important pathogen that exhibit high metabolic versatility allowing it to infect various niches within a host. S. aureus utilizes two major transcriptional regulators, CodY and CcpA, to remodel metabolic and virulence gene expression in response to changing environmental conditions. Previous studies revealed that inactivation of either codY or ccpA has a pronounced impact on different aspects of staphylococcal physiology and pathogenesis. To determine the contribution and interplay of these two regulators in modulating central metabolism, virulence, and biofilm development we constructed and characterized codY ccpA double mutant in S. aureus UAMS-1. In line with previous studies, we found that CcpA and CodY control cellular metabolic status by altering carbon flow through the central and overflow metabolic pathways. Our results demonstrate that ccpA inactivation impairs biofilm formation and decreases incorporation of eDNA into the biofilm matrix while disrupting codY resulted in a robust structured biofilm tethered together with eDNA and PIA. Interestingly, inactivation of both codY and ccpA decreases biofilm biomass and neglects eDNA release in the double mutant. Compared to inactivation of codY, the codY ccpA mutant did not overexpress toxins but maintained overexpression of amino acid metabolism pathways. Furthermore, codY ccpA mutant produced higher amounts of PIA, in contrast to the wild-type strain and ccpA mutant. Overall, results of this study suggest that interplay between CodY and CcpA modulates central metabolism to optimize growth on preferred carbon sources while repressing virulence gene expression until nutrient limitation requires scavenging nutrients from the host.
University of Nebraska Medical Center
DepartmentPathology and Microbiology
Last NameSadykov
First NameMarat
AddressUNMC Department of Pathology and Microbiology 985900 Nebraska Medical Center Omaha, NE 68198-5900
Submit Date2022-05-25
Raw Data AvailableYes
Raw Data File Type(s)mzML
Analysis Type DetailLC-MS
Release Date2022-06-14
Release Version1
Marat Sadykov Marat Sadykov application/zip

Select appropriate tab below to view additional metadata details:


Subject type: Bacteria; Subject species: Staphylococcus aureus (Factor headings shown in green)

mb_sample_id local_sample_id Genotype
SA210074codY 3h_3ccpA
SA210075ccpA 6h_1ccpA
SA210076codY 3h_2ccpA
SA210077codY 3h_1ccpA
SA210078ccpA 3h_1ccpA
SA210079ccpA 6h_3ccpA
SA210080ccpA 6h_2ccpA
SA210081ccpA 3h_2ccpA
SA210082ccpA 3h_3ccpA
SA210083codY 6h_2ccpA
SA210084codY 6h_1ccpA
SA210085codY 6h_3ccpA
SA210086codY ccpA 6h_1codY ccpA
SA210087codY ccpA 6h_2codY ccpA
SA210088codY ccpA 6h_3codY ccpA
SA210089codY ccpA 3h_1codY ccpA
SA210090codY ccpA 3h_2codY ccpA
SA210091codY ccpA 3h_3codY ccpA
SA210068WT 6h_2WT
SA210069WT 3h_3WT
SA210070WT 3h_2WT
SA210071WT 6h_1WT
SA210072WT 6h_3WT
SA210073WT 3h_1WT
Showing results 1 to 24 of 24