Summary of Study ST002222

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001418. The data can be accessed directly via it's Project DOI: 10.21228/M8F409 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002222
Study TitleGlutaminolysis contribution to the carbon backbone of aspartate and glutamate in ccRCC
Study SummaryThe objective of this experiment is to test the contribution of the carbons derived from glutamine to the generation of aspartate and glutamate in human epithelial renal cells HK2 and ccRCC cell lines 786-O and 786-M1A. To test this hypothesis, we incubated all cells with 13C5-glutamine in Plasmax media with or without a pharmacological inhibitor of glutaminase CB-839. This is Part 7 of a study and the experimental number is MS57.
Institute
CECAD Research Center
Last NameYang
First NameMing
AddressJoseph-Stelzmann-Straße 26, Köln, Koeln, 50931, Germany
Emailming.yang@uni-koeln.de
Phone4922147884306
Submit Date2022-07-15
Raw Data AvailableYes
Raw Data File Type(s)raw(Thermo)
Analysis Type DetailLC-MS
Release Date2022-08-03
Release Version1
Ming Yang Ming Yang
https://dx.doi.org/10.21228/M8F409
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Factors:

Subject type: Cultured cells; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Cell line Treatment
SA212017MS57_049786M1A CB839 100nM
SA212018MS57_048786M1A CB839 100nM
SA212019MS57_047786M1A CB839 100nM
SA212020MS57_050786M1A CB839 100nM
SA212021MS57_046786M1A CB839 100nM
SA212022MS57_057786M1A CB839 1μM
SA212023MS57_058786M1A CB839 1μM
SA212024MS57_059786M1A CB839 1μM
SA212025MS57_060786M1A CB839 1μM
SA212026MS57_056786M1A CB839 1μM
SA212027MS57_051786M1A CB839 500nM
SA212028MS57_055786M1A CB839 500nM
SA212029MS57_052786M1A CB839 500nM
SA212030MS57_054786M1A CB839 500nM
SA212031MS57_053786M1A CB839 500nM
SA212032MS57_042786M1A DMSO
SA212033MS57_041786M1A DMSO
SA212034MS57_043786M1A DMSO
SA212035MS57_044786M1A DMSO
SA212036MS57_045786M1A DMSO
SA212037MS57_027786O CB839 100nM
SA212038MS57_028786O CB839 100nM
SA212039MS57_030786O CB839 100nM
SA212040MS57_029786O CB839 100nM
SA212041MS57_026786O CB839 100nM
SA212042MS57_037786O CB839 1μM
SA212043MS57_036786O CB839 1μM
SA212044MS57_038786O CB839 1μM
SA212045MS57_040786O CB839 1μM
SA212046MS57_039786O CB839 1μM
SA212047MS57_035786O CB839 500nM
SA212048MS57_034786O CB839 500nM
SA212049MS57_031786O CB839 500nM
SA212050MS57_033786O CB839 500nM
SA212051MS57_032786O CB839 500nM
SA212052MS57_022786O DMSO
SA212053MS57_021786O DMSO
SA212054MS57_023786O DMSO
SA212055MS57_024786O DMSO
SA212056MS57_025786O DMSO
SA212057MS57_008HK2 CB839 100nM
SA212058MS57_007HK2 CB839 100nM
SA212059MS57_010HK2 CB839 100nM
SA212060MS57_006HK2 CB839 100nM
SA212061MS57_009HK2 CB839 100nM
SA212062MS57_020HK2 CB839 1μM
SA212063MS57_019HK2 CB839 1μM
SA212064MS57_018HK2 CB839 1μM
SA212065MS57_017HK2 CB839 1μM
SA212066MS57_016HK2 CB839 1μM
SA212067MS57_015HK2 CB839 500nM
SA212068MS57_011HK2 CB839 500nM
SA212069MS57_014HK2 CB839 500nM
SA212070MS57_013HK2 CB839 500nM
SA212071MS57_012HK2 CB839 500nM
SA212072MS57_002HK2 DMSO
SA212073MS57_003HK2 DMSO
SA212074MS57_001HK2 DMSO
SA212075MS57_005HK2 DMSO
SA212076MS57_004HK2 DMSO
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