Summary of Study ST002251

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001439. The data can be accessed directly via it's Project DOI: 10.21228/M8QD9Z This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002251
Study TitleUntargeted metabolomics on plasma from children with asthma, comparing exacerbation-prone to non-exacerbation-prone
Study TypeUntargeted MS
Study SummaryBackground: Some children with asthma remain poorly controlled and have recurrent exacerbations despite treatment with inhaled corticosteroids. Aside from prior exacerbations, there are currently no reliable predictors of exacerbation-prone asthma in these children and limited understanding of potential underlying mechanisms. Objective: We sought to quantify small molecules in the plasma of children with exacerbation- prone asthma through mass spectrometry-based metabolomics. We hypothesized that the plasma metabolome of these children would differ from that of children with non-exacerbation- prone asthma. Methods: Plasma metabolites were extracted from four pediatric asthma cohorts (n=215 total, n=41 with exacerbation-prone asthma) and detected using a ZIC-HILIC column coupled to a Q Exactive HF mass spectrometer. High-confidence annotations were retained for univariate analysis and were confirmed by a sensitivity analysis in subjects on high-dose inhaled corticosteroids. Metabolites that varied by cohort were excluded. Metaboanalyst was used to identify pathways of interest. Concentrations were calculated by reference standardization to NIST SRM 1950. Results: We identified 32 unique, cohort-independent metabolites that differed in children with exacerbation-prone asthma compared to children with non-exacerbation-prone asthma. Comparison of metabolite concentrations to literature-reported values for healthy children revealed that most metabolites were decreased in both asthma groups, but more so in exacerbation-prone asthma. Pathway analysis identified arginine, lysine, and methionine pathways as most impacted. Conclusions: Several plasma metabolites are perturbed in children with exacerbation-prone asthma and are largely related to arginine, lysine, and methionine pathways. While validation is needed, plasma metabolites may be potential biomarkers for exacerbation-prone asthma in children.
Institute
Emory University
DepartmentPediatrics
LaboratoryJoshua Chandler, PhD
Last NameChandler
First NameJoshua
Address2015 Upper Gate Drive NE, Atlanta, GA 30322
Emailjoshua.chandler@emory.edu
Phone404-727-3536
Submit Date2022-07-25
Num Groups2
Total Subjects215
Num Males135
Num Females80
PublicationsJACI-D-22-00220
Raw Data AvailableYes
Raw Data File Type(s)mzML
Analysis Type DetailLC-MS
Release Date2023-01-02
Release Version1
Joshua Chandler Joshua Chandler
https://dx.doi.org/10.21228/M8QD9Z
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id ThreeorMoreBursts ICShighdose Study AgeatEnrollment AsthmaDurationYears Sex Ethnicity Race Height Weight BMI LABA Montelukast
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SA21679420200725_005_QC-ddMS2-posn/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a
SA21679520200720_003_QC-posn/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a
SA21679620200725_003_QC-posn/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a
SA21679720200725_001_Blank-posn/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a
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SA21680320200718_006_QC-ddMS2-negn/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a
SA21680420200718_005_QC-ddMS2-posn/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a
SA21680520200720_027_QC-posn/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a
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SA21688820200717_050_QC-negn/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a
SA21688920200717_049_QC-posn/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a
SA21689020200720_050_QC-negn/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a
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