Summary of Study ST002379

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001524. The data can be accessed directly via it's Project DOI: 10.21228/M8Q13W This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002379
Study TitleGlucose flux analysis of NLRP3 inflammasome activated macrophages
Study TypeBasic research
Study SummaryActivating macrophage NLRP3 inflammasome can promote excessive inflammation, with severe cell and tissue damage and organ dysfunction. Here, we show that pharmacological or genetic inhibition of pyruvate dehydrogenase kinase (PDHK) significantly attenuates NLRP3 inflammasome activation in murine and human macrophages and septic mice by lowering caspase-1 cleavage and IL-1beta secretion. Inhibiting PDHK reverses NLRP3 inflammasome-induced metabolic reprogramming, enhances autophagy, promotes mitochondrial fusion over fission, preserves cristae ultrastructure, and attenuates mitochondrial ROS production. The suppressive effect of PDHK inhibition on the NLRP3 inflammasome is independent of its canonical role as a pyruvate dehydrogenase regulator. We suggest that PDHK inhibition improves mitochondrial fitness by reversing NLRP3 inflammasome activation in acutely inflamed macrophages.
Institute
Wake Forest School of Medicine
Last NameZhu
First NameXuewei
Address575 Patterson Ave, Winston-Salem, NC 27101
Emailxwzhu@wakehealth.edu
Phone3367131445
Submit Date2022-11-15
Raw Data AvailableYes
Raw Data File Type(s)raw(Thermo)
Analysis Type DetailLC-MS
Release Date2022-12-15
Release Version1
Xuewei Zhu Xuewei Zhu
https://dx.doi.org/10.21228/M8Q13W
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Factors:

Subject type: Cultured cells; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Treatment
SA237431LPS_JX06_ATP_2JPS+JX06+ATP
SA237432LPS_JX06_ATP_1JPS+JX06+ATP
SA237433LPS_JX06_ATP_3JPS+JX06+ATP
SA237434LPS_3LPS
SA237435LPS_1LPS
SA237436LPS_2LPS
SA237437LPS_ATP_3LPS+ATP
SA237438LPS_ATP_1LPS+ATP
SA237439LPS_ATP_2LPS+ATP
SA237440Ctrl_C_2No treatment
SA237441Ctrl_C_1No treatment
SA237442Ctrl_C_3No treatment
Showing results 1 to 12 of 12
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