Summary of Study ST002391
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001538. The data can be accessed directly via it's Project DOI: 10.21228/M8WH85 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002391 |
| Study Title | Evaluation of Two Simultaneous Metabolomic and Proteomic Extraction Protocols Assessed by Ultra-High-Performance Liquid Chromatography Tandem Mass Spectrometry |
| Study Summary | Untargeted multi-omics analysis of plasma is an emerging tool for the identification of novel biomarkers for evaluating disease prognosis and for a better understanding of molecular mechanisms underlying human disease. The successful application of metabolomic and proteomic approaches relies on reproducibly quantifying a wide range of metabolites and proteins. Herein, we report the results of untargeted metabolomic and proteomic analyses from blood plasma samples following analyte extraction by two frequently used solvent systems: chloro-form/methanol and methanol-only. Whole blood samples were collected from participants (n=6) at University Hospital Sharjah (UHS) hospital, then plasma was separated and extracted by two methods i. methanol precipitation and, ii. 4:3 methanol:chloroform extraction. The coverage and reproducibility of the two methods were assessed by ultra-high-performance liquid chromatography-electrospray ionization quadrupole time-of-flight mass spectrometry (UHPLC-ESI-QTOF-MS). The study revealed that metabolite extraction by methanol-only showed greater reproducibility for both metabolomic and proteomic quantifications than did methanol/chloroform, while yielding similar peptide coverage. However, coverage of extracted metabolites was higher with the methanol/chloroform precipitation. |
| Institute | Sharjah Institute for Medical Research |
| Last Name | Facility |
| First Name | Core |
| Address | M32, SIMR, College of Pharmacy, Health Sciences, University of Sharjah |
| tims-tof@sharjah.ac.ae | |
| Phone | 065057656 |
| Submit Date | 2022-12-06 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | d |
| Analysis Type Detail | LC-MS |
| Release Date | 2023-01-06 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Factors:
Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)
| mb_sample_id | local_sample_id | Method |
|---|---|---|
| SA238175 | A02-02-4395 | Methanol;Chloroform |
| SA238176 | A03-01-4396 | Methanol;Chloroform |
| SA238177 | A04-01-4398 | Methanol;Chloroform |
| SA238178 | A02-01-4394 | Methanol;Chloroform |
| SA238179 | A03-02-4397 | Methanol;Chloroform |
| SA238180 | RA04-02-4399 | Methanol;Chloroform |
| SA238181 | A01-02-4393 | Methanol;Chloroform |
| SA238182 | RB04-02-4417 | Methanol;Chloroform |
| SA238183 | RB04-01-4416 | Methanol;Chloroform |
| SA238184 | A04-02-4399 | Methanol;Chloroform |
| SA238185 | RA04-01-4398 | Methanol;Chloroform |
| SA238186 | RA03-01-4408 | Methanol;Chloroform |
| SA238187 | A05-02-4401 | Methanol;Chloroform |
| SA238188 | A06-01-4402 | Methanol;Chloroform |
| SA238189 | A01-01-4392 | Methanol;Chloroform |
| SA238190 | A06-02-4403 | Methanol;Chloroform |
| SA238191 | A05-01-4400 | Methanol;Chloroform |
| SA238192 | RA01-01-4404 | Methanol;Chloroform |
| SA238193 | RA02-02-4407 | Methanol;Chloroform |
| SA238194 | RA02-01-4406 | Methanol;Chloroform |
| SA238195 | RA01-02-4405 | Methanol;Chloroform |
| SA238196 | RA03-02-4409 | Methanol;Chloroform |
| SA238157 | B05-02-4419 | Methanol Only |
| SA238158 | B06-01-4420 | Methanol Only |
| SA238159 | B04-02-4417 | Methanol Only |
| SA238160 | B03-02-4415 | Methanol Only |
| SA238161 | B03-01-4414 | Methanol Only |
| SA238162 | B06-02-4421 | Methanol Only |
| SA238163 | B04-01-4416 | Methanol Only |
| SA238164 | RB01-01-4422 | Methanol Only |
| SA238165 | RB03-01-4426 | Methanol Only |
| SA238166 | RB03-02-4427 | Methanol Only |
| SA238167 | RB02-02-4425 | Methanol Only |
| SA238168 | RB02-01-4424 | Methanol Only |
| SA238169 | RB01-02-4423 | Methanol Only |
| SA238170 | B02-02-4413 | Methanol Only |
| SA238171 | B05-01-4418 | Methanol Only |
| SA238172 | B02-01-4412 | Methanol Only |
| SA238173 | B01-01-4410 | Methanol Only |
| SA238174 | B01-02-4411 | Methanol Only |
| Showing results 1 to 40 of 40 |
