Summary of Study ST002421

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001559. The data can be accessed directly via it's Project DOI: 10.21228/M85X3W This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002421
Study TitleUBXD8 lipidomics from whole cells (Part 1)
Study SummaryThe intimate association between the endoplasmic reticulum (ER) and mitochondrial membranes at ER-mitochondria contact sites (ERMCS) serves as a platform for several critical cellular processes, in particular lipid synthesis. Enzymes involved in lipid biosynthesis are enriched at contacts and membrane lipid composition at contacts is distinct relative to surrounding membranes. How contacts are remodeled and the subsequent biological consequences of altered contacts such as perturbed lipid metabolism remains poorly understood. Here we investigate if the ER-tethered ubiquitin-X domain adaptor 8 (UBXD8) regulates the lipidome of cells. LC-MS/MS lipidomics found significant changes in distinct lipid species in UBXD8 knockout cells, in particular in saturated or mono-unsaturated lipid species. Perturbation of contacts and inherent lipid synthesis is emerging as a hallmark in a variety of human disorders such as neurodegeneration. Our results suggest that contacts are exquisitely sensitive to alterations to membrane lipid composition and saturation in a manner that is dependent on UBXD8.
Institute
University of Arizona
DepartmentImmunobiology
LaboratoryPurdy Lab
Last NamePurdy
First NameJohn
AddressPO Box 245221, Tucson, Arizona, 85724, USA
Emailpurdylab@gmail.com
Phone520-626-4371
Submit Date2022-12-30
Raw Data AvailableYes
Raw Data File Type(s)mzXML
Analysis Type DetailLC-MS
Release Date2023-01-16
Release Version1
John Purdy John Purdy
https://dx.doi.org/10.21228/M85X3W
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Factors:

Subject type: Cultured cells; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Description
SA24234720191130_pos_rep3_KO_aUBXD8 Knockout
SA24234820191130_pos_rep2_KO_bUBXD8 Knockout
SA24234920191130_neg_rep3_KO_bUBXD8 Knockout
SA24235020191130_pos_rep3_KO_bUBXD8 Knockout
SA24235120190829_neg_KO_1UBXD8 Knockout
SA24235220191230_neg_rep2_KO_aUBXD8 Knockout
SA24235320191230_pos_rep2_KO_bUBXD8 Knockout
SA24235420191230_pos_rep2_KO_aUBXD8 Knockout
SA24235520191230_neg_rep2_KO_bUBXD8 Knockout
SA24235620191130_neg_rep3_KO_aUBXD8 Knockout
SA24235720191130_pos_rep2_KO_aUBXD8 Knockout
SA24235820190829_pos_KO_2UBXD8 Knockout
SA24235920190829_pos_KO_1UBXD8 Knockout
SA24236020191130_neg_rep2_KO_bUBXD8 Knockout
SA24236120191130_neg_rep2_KO_aUBXD8 Knockout
SA24236220190829_neg_KO_2UBXD8 Knockout
SA24236320191230_neg_rep2_WT_aWild-type
SA24236420191230_neg_rep2_WT_bWild-type
SA24236520190829_neg_wt_2Wild-type
SA24236620191230_pos_rep2_WT_bWild-type
SA24236720190829_neg_wt_1Wild-type
SA24236820191230_pos_rep2_WT_aWild-type
SA24236920191130_pos_rep3_WT_aWild-type
SA24237020191130_neg_rep3_WT_bWild-type
SA24237120191130_neg_rep3_WT_aWild-type
SA24237220191130_neg_rep2_WT_aWild-type
SA24237320191130_pos_rep2_WT_aWild-type
SA24237420191130_pos_rep2_WT_bWild-type
SA24237520191130_neg_rep2_WT_bWild-type
SA24237620190829_pos_wt_1Wild-type
SA24237720190829_pos_wt_2Wild-type
SA24237820191130_pos_rep3_WT_bWild-type
Showing results 1 to 32 of 32
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