Summary of Study ST002422

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001559. The data can be accessed directly via it's Project DOI: 10.21228/M85X3W This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002422
Study TitleUBXD8 lipidomics from whole cells (Part 2)
Study SummaryThe intimate association between the endoplasmic reticulum (ER) and mitochondrial membranes at ER-mitochondria contact sites (ERMCS) serves as a platform for several critical cellular processes, in particular lipid synthesis. Enzymes involved in lipid biosynthesis are enriched at contacts and membrane lipid composition at contacts is distinct relative to surrounding membranes. How contacts are remodeled and the subsequent biological consequences of altered contacts such as perturbed lipid metabolism remains poorly understood. Here we investigate if the ER-tethered ubiquitin-X domain adaptor 8 (UBXD8) regulates the lipids found in mitochondria-associated membranes (MAM). LC-MS/MS lipidomics found significant changes in distinct lipid species in the MAM fraction of UBXD8 knockout cells. Our results suggest that lipids in MAM are regulated by UBXD8.
Institute
University of Arizona
DepartmentImmunobiology
LaboratoryPurdy Lab
Last NamePurdy
First NameJohn
AddressPO Box 245221, Tucson, Arizona, 85724, USA
Emailpurdylab@gmail.com
Phone520-626-4371
Submit Date2023-01-01
Raw Data AvailableYes
Raw Data File Type(s)mzXML
Analysis Type DetailLC-MS
Release Date2023-01-16
Release Version1
John Purdy John Purdy
https://dx.doi.org/10.21228/M85X3W
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Factors:

Subject type: Cultured cells; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Description inj vol
SA24237920220820_tufts_MAMfrac_neg_rep8_KO_10ulUBXD8 Knockout 10
SA24238020220508_neg_KO_rep4_10ulUBXD8 Knockout 10
SA24238120220508_neg_KO_rep3_10ulUBXD8 Knockout 10
SA24238220220508_neg_KO_rep1_10ulUBXD8 Knockout 10
SA24238320220820_tufts_MAMfrac_neg_rep8_KO_15ulUBXD8 Knockout 15
SA24238420220508_pos_KO_rep3_16ulUBXD8 Knockout 16
SA24238520220820_tufts_MAMfrac_pos_rep8_KO_16ulUBXD8 Knockout 16
SA24238620220508_pos_KO_rep4_16ulUBXD8 Knockout 16
SA24238720220508_pos_KO_rep1_16ulUBXD8 Knockout 16
SA24238820220820_tufts_MAMfrac_pos_rep8_KO_4ulUBXD8 Knockout 4
SA24238920220508_pos_KO_rep3_4ulUBXD8 Knockout 4
SA24239020220508_pos_KO_rep1_4ulUBXD8 Knockout 4
SA24239120220508_pos_KO_rep4_4ulUBXD8 Knockout 4
SA24239220220820_tufts_MAMfrac_neg_rep8_KO_5ulUBXD8 Knockout 5
SA24239320220508_pos_KO_rep1_8ulUBXD8 Knockout 8
SA24239420220508_pos_KO_rep4_8ulUBXD8 Knockout 8
SA24239520220820_tufts_MAMfrac_pos_rep8_KO_8ulUBXD8 Knockout 8
SA24239620220508_pos_KO_rep3_8ulUBXD8 Knockout 8
SA24239720220508_neg_WT_rep3_10ulWild-type 10
SA24239820220508_neg_WT_rep4_10ulWild-type 10
SA24239920220508_neg_WT_rep1_10ulWild-type 10
SA24240020220820_tufts_MAMfrac_neg_rep8_WT_10ulWild-type 10
SA24240120220820_tufts_MAMfrac_neg_rep8_WT_15ulWild-type 15
SA24240220220508_pos_WT_rep3_16ulWild-type 16
SA24240320220508_pos_WT_rep1_16ulWild-type 16
SA24240420220508_pos_WT_rep4_16ulWild-type 16
SA24240520220820_tufts_MAMfrac_pos_rep8_WT_16ulWild-type 16
SA24240620220820_tufts_MAMfrac_pos_rep8_WT_4ulWild-type 4
SA24240720220508_pos_WT_rep3_4ulWild-type 4
SA24240820220508_pos_WT_rep4_4ulWild-type 4
SA24240920220508_pos_WT_rep1_4ulWild-type 4
SA24241020220820_tufts_MAMfrac_neg_rep8_WT_5ulWild-type 5
SA24241120220508_pos_WT_rep1_8ulWild-type 8
SA24241220220508_pos_WT_rep3_8ulWild-type 8
SA24241320220508_pos_WT_rep4_8ulWild-type 8
SA24241420220820_tufts_MAMfrac_pos_rep8_WT_8ulWild-type 8
Showing results 1 to 36 of 36
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