Summary of Study ST002438

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001570. The data can be accessed directly via it's Project DOI: 10.21228/M8RM66 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002438
Study TitleOzone alters glycosphingolipid metabolism and exacerbates characteristics of asthma in mice
Study SummaryAsthma is a common chronic respiratory disease exacerbated by multiple environmental factors, including exposure to air pollutants such as ozone. Acute ozone exposure has previously been implicated in airway inflammation, airway hyperreactivity, and other characteristics of asthma. Altered sphingolipid metabolism following ozone exposure may contribute to the molecular mechanisms underlying these previously reported effects. This study aimed to identify changes in metabolomic profiles and characteristics of asthma in allergen-sensitized mice following ozone exposure to provide insights regarding mechanisms of ozone-induced exacerbations in asthma. Adult male and female BALB/c mice were sensitized intranasally to house dust mite allergen (HDM) on days 1, 3, and 5 followed by HDM challenge on days 12-14. Mice were subsequently exposed to ozone following each HDM challenge for 6 hr/day. Bronchoalveolar lavage, plasma, whole lung lobes, and microdissected lung airways were collected from 8 female and 8 male mice for metabolomics analysis. 6 female and 6 male mice underwent methacholine challenge using a forced oscillation technique to assess pulmonary function. HDM-sensitized male mice exposed to ozone displayed synergistically increased airway hyperreactivity as well as increased airway inflammation and eosinophilia relative to control mice. Effects in male mice were significantly more severe than the effects observed in females. Both HDM-sensitized male and female mice exposed to ozone displayed significant decreases in multiple classes of sphingolipids in microdissected airways. However, glycosphingolipids were significantly increased in females and to a lesser extent in males. These results potentially implicate glycosphingolipids in protecting against severe outcomes of ozone exposure that coincide with exacerbation of allergic asthma.
Institute
University of California, Davis
Last NameStevens
First NameNathanial
Address451 Health Sciences Drive
Emailncstevens@ucdavis.edu
Phone8282844315
Submit Date2022-08-25
Raw Data AvailableYes
Raw Data File Type(s)raw(Thermo)
Analysis Type DetailLC-MS
Release Date2023-01-25
Release Version1
Nathanial Stevens Nathanial Stevens
https://dx.doi.org/10.21228/M8RM66
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Factors:

Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Factor
SA243615mx626289_OzAW_BR3BR
SA243616mx626289_OzAW_BR1BR
SA243617mx626289_OzAW_BR4BR
SA243618mx626289_OzAW_BR2BR
SA243619mx626289_OzAW_BR7BR
SA243620mx626289_OzAW_BR6BR
SA243621mx626289_OzAW_BR5BR
SA243622mx626289_OzAW_75HFA_F
SA243623mx626289_OzAW_74HFA_F
SA243624mx626289_OzAW_76HFA_F
SA243625mx626289_OzAW_80HFA_F
SA243626mx626289_OzAW_73HFA_F
SA243627mx626289_OzAW_79HFA_F
SA243628mx626289_OzAW_78HFA_F
SA243629mx626289_OzAW_77HFA_F
SA243630mx626289_OzAW_65HFA_M
SA243631mx626289_OzAW_70HFA_M
SA243632mx626289_OzAW_71HFA_M
SA243633mx626289_OzAW_66HFA_M
SA243634mx626289_OzAW_67HFA_M
SA243635mx626289_OzAW_69HFA_M
SA243636mx626289_OzAW_68HFA_M
SA243637mx626289_OzAW_72HFA_M
SA243638mx626289_OzAW_89HO3_F
SA243639mx626289_OzAW_94HO3_F
SA243640mx626289_OzAW_95HO3_F
SA243641mx626289_OzAW_96HO3_F
SA243642mx626289_OzAW_93HO3_F
SA243643mx626289_OzAW_92HO3_F
SA243644mx626289_OzAW_90HO3_F
SA243645mx626289_OzAW_91HO3_F
SA243646mx626289_OzAW_83HO3_M
SA243647mx626289_OzAW_82HO3_M
SA243648mx626289_OzAW_86HO3_M
SA243649mx626289_OzAW_88HO3_M
SA243650mx626289_OzAW_87HO3_M
SA243651mx626289_OzAW_81HO3_M
SA243652mx626289_OzAW_85HO3_M
SA243653mx626289_OzAW_84HO3_M
SA243654mx626289_OzAW_MB06MB
SA243655mx626289_OzAW_MB07MB
SA243656mx626289_OzAW_MB05MB
SA243657mx626289_OzAW_MB04MB
SA243658mx626289_OzAW_MB01MB
SA243659mx626289_OzAW_MB02MB
SA243660mx626289_OzAW_MB03MB
SA243661mx626289_OzAW_P1P
SA243662mx626289_OzAW_P3P
SA243663mx626289_OzAW_P2P
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SA243665mx626289_OzAW_P7P
SA243666mx626289_OzAW_P4P
SA243667mx626289_OzAW_P6P
SA243668mx626289_OzAW_16SFA_F
SA243669mx626289_OzAW_11SFA_F
SA243670mx626289_OzAW_10SFA_F
SA243671mx626289_OzAW_15SFA_F
SA243672mx626289_OzAW_14SFA_F
SA243673mx626289_OzAW_12SFA_F
SA243674mx626289_OzAW_9SFA_F
SA243675mx626289_OzAW_13SFA_F
SA243676mx626289_OzAW_1SFA_M
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SA243678mx626289_OzAW_5SFA_M
SA243679mx626289_OzAW_4SFA_M
SA243680mx626289_OzAW_3SFA_M
SA243681mx626289_OzAW_7SFA_M
SA243682mx626289_OzAW_8SFA_M
SA243683mx626289_OzAW_2SFA_M
SA243684mx626289_OzAW_26SO3_F
SA243685mx626289_OzAW_29SO3_F
SA243686mx626289_OzAW_30SO3_F
SA243687mx626289_OzAW_32SO3_F
SA243688mx626289_OzAW_28SO3_F
SA243689mx626289_OzAW_31SO3_F
SA243690mx626289_OzAW_25SO3_F
SA243691mx626289_OzAW_27SO3_F
SA243692mx626289_OzAW_18SO3_M
SA243693mx626289_OzAW_19SO3_M
SA243694mx626289_OzAW_20SO3_M
SA243695mx626289_OzAW_22SO3_M
SA243696mx626289_OzAW_24SO3_M
SA243697mx626289_OzAW_21SO3_M
SA243698mx626289_OzAW_17SO3_M
SA243699mx626289_OzAW_23SO3_M
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