Summary of Study ST002524

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001626. The data can be accessed directly via it's Project DOI: 10.21228/M8HT4B This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

Perform statistical analysis  |  Show all samples  |  Show named metabolites  |  Download named metabolite data  
Download mwTab file (text)   |  Download mwTab file(JSON)   |  Download data files (Contains raw data)
Study IDST002524
Study TitleIn situ microwave fixation provides an instantaneous snapshot of the brain metabolome - Part 1
Study SummaryWe demonstrate exhaustion of glycogen and glucose and an increase in lactate production during conventional rapid brain resection prior to preservation by liquid nitrogen that is not observed with microwave fixation. Next, microwave fixation was employed to define the impact of brain glucose metabolism in the mouse model of streptozotocin-induced type 1 diabetes. Using both total pool and isotope tracing analyses, we identified global glucose hypometabolism in multiple regions of the mouse brain, evidenced by reduced 13C enrichment into glycogen, glycolysis, and the TCA cycle. Reduced glucose metabolism correlated with a marked decrease in GLUT2 expression and several metabolic enzymes in unique brain regions. In conclusion, our study supports the incorporation of microwave fixation to study terminal brain metabolism in rodent models.
Institute
University of Florida
Last NameSun
First NameRamon
Address1200 Newell Drive, ARB
Emailramonsun@ufl.edu
Phone3522948407
Submit Date2023-03-22
Raw Data AvailableYes
Raw Data File Type(s)cdf
Analysis Type DetailGC-MS
Release Date2023-04-17
Release Version1
Ramon Sun Ramon Sun
https://dx.doi.org/10.21228/M8HT4B
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Factors:

Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Tissue Region preservation method
SA254517CTX CP 2cortex cryopreservation
SA254518CTX CP 1cortex cryopreservation
SA254519CTX CP 3cortex cryopreservation
SA254520CTX HPFM 2cortex focused microwave
SA254521CTX HPFM 1cortex focused microwave
SA254522CTX HPFM 3cortex focused microwave
SA254523DVC CP 2dorsal vagal complex cryopreservation
SA254524DVC CP 3dorsal vagal complex cryopreservation
SA254525DVC CP 1dorsal vagal complex cryopreservation
SA254526DVC HPFM 1dorsal vagal complex focused microwave
SA254527DVC HPFM 2dorsal vagal complex focused microwave
SA254528DVC HPFM 3dorsal vagal complex focused microwave
SA254511Hipp CP 1Hippocampus cryopreservation
SA254512Hipp CP 3Hippocampus cryopreservation
SA254513Hipp CP 2Hippocampus cryopreservation
SA254514Hipp HPFM 2Hippocampus focused microwave
SA254515Hipp HPFM 3Hippocampus focused microwave
SA254516Hipp HPFM 1Hippocampus focused microwave
Showing results 1 to 18 of 18
  logo