Summary of Study ST002580

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001020. The data can be accessed directly via it's Project DOI: 10.21228/M8V97D This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002580
Study TitleMoTrPAC: Endurance exercise training study in young adult rats, Tissue:Plasma - Targeted Ethanolamides
Study SummaryThe goal of the endurance exercise training study in young adult rats (internal code: PASS1B-06) was to perform exercise training studies in adult (6 month) F344 rats, and from these rats collect as many tissues as feasible in order to provide high quality samples for detailed analysis by chemical analysis sites. Tissues were collected from 10-12 rats sedentary control rats concurrent with the collection of the 8-week training groups. The 8-week training group and controls were from the same cohort and same age at euthanasia (either 8). For the older age group, an additional set of controls (n=5-6) were collected with the 1-2 week training group. Rats were either sedentary or underwent an exercise training program. Rats were exercised on the rodent treadmill 5 days per week using a progressive training protocol designed to exercise the rats at approximately 70% of VO2max as outlined in the Table on the next page. Training was performed no earlier than 10:00 am and no later than 5:00 pm over 5 consecutive days per week. Training was initiated with the treadmill set at 70% of VO2 max (see tables) and 5 degrees grade for 20 minutes. The duration of exercise was increased by one minute each day until day 31 of training (start of week 7), when a duration of 50 min was reached. Speed and grade of each training session increased in larger increments due to treadmill parameters. The highest intensity and duration of training began on day 31. This intensity was maintained for the final 10 days of the protocol to ensure steady state had been achieved. If any rats were unable to perform at least 4 days of training per week they were removed from the study and euthanized. It is important to note that the starting treadmill speed varied depending on the sex and age of the rat. The initial and maximum speeds were based on VO2max measurements obtained during the pre-training testing of the compliant rats. Rats assigned to the control group followed a schedule similar to the training group. They were placed in one lane on the treadmill for 15 minutes/day, 5 days per week. The treadmill was set at 0 m/min at an incline that corresponded to the incline being used by the training group.
Institute
Emory University
DepartmentBiochemistry
LaboratoryIntegrated Lipidomics and Metabolomics Core
Last NameOrtlund
First NameEric
Address1510 Clifton Rd. NE, Room G235, Atlanta, GA 30322
Emaileortlun@emory.edu
Phone(404) 727-5014
Submit Date2023-04-25
Raw Data AvailableYes
Raw Data File Type(s)TBD
Analysis Type DetailLC-MS
Release Date2023-10-06
Release Version1
Eric Ortlund Eric Ortlund
https://dx.doi.org/10.21228/M8V97D
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:


Factors:

Subject type: Mammal; Subject species: Rattus norvegicus (Factor headings shown in green)

mb_sample_id local_sample_id Group Timepoint Sex
SA25889990266013107Control 8 weeks of training or control time Female
SA25890090252013107Control 8 weeks of training or control time Female
SA25890190265013107Control 8 weeks of training or control time Female
SA25890290245013107Control 8 weeks of training or control time Female
SA25890390248013107Control 8 weeks of training or control time Female
SA25890490237013107Control 8 weeks of training or control time Male
SA25890590229013107Control 8 weeks of training or control time Male
SA25890690217013107Control 8 weeks of training or control time Male
SA25890790239013107Control 8 weeks of training or control time Male
SA25890890232013107Control 8 weeks of training or control time Male
SA258909QC_blank_79QC-Blank 0 hour --
SA258910QC_blank_78QC-Blank 0 hour --
SA258911QC_blank_65QC-Blank 0 hour --
SA258912QC_blank_68QC-Blank 0 hour --
SA258913QC_blank_4QC-Blank 0 hour --
SA258914QC_blank_69QC-Blank 0 hour --
SA258915QC_Standard_76QC-ExternalStandard 0 hour --
SA258916QC_Standard_72QC-ExternalStandard 0 hour --
SA258917QC_Standard_73QC-ExternalStandard 0 hour --
SA258918QC_Standard_71QC-ExternalStandard 0 hour --
SA258919QC_Standard_77QC-ExternalStandard 0 hour --
SA258920QC_Standard_70QC-ExternalStandard 0 hour --
SA258921QC_Standard_75QC-ExternalStandard 0 hour --
SA258922QC_Standard_74QC-ExternalStandard 0 hour --
SA258923QC_62QC-Pooled 0 hour --
SA258924QC_67QC-Pooled 0 hour --
SA258925QC_51QC-Pooled 0 hour --
SA258926QC_40QC-Pooled 0 hour --
SA258927QC_7QC-Pooled 0 hour --
SA258928QC_18QC-Pooled 0 hour --
SA258929QC_29QC-Pooled 0 hour --
SA258930QC_prerun_1QC-PreRun 0 hour --
SA258931QC_prerun_2QC-PreRun 0 hour --
SA258932QC_prerun_3QC-PreRun 0 hour --
SA258933G_QC_2_64QC-Reference 0 hour --
SA258934G_QC_2_6QC-Reference 0 hour --
SA258935G_QC_1_5QC-Reference 0 hour --
SA258936G_QC_1_63QC-Reference 0 hour --
SA258937QC_SRMQC-Reference 0 hour --
SA25893890564013107Training 1 week of training or control time Female
SA25893990560013107Training 1 week of training or control time Female
SA25894090571013107Training 1 week of training or control time Female
SA25894190567013107Training 1 week of training or control time Female
SA25894290559013107Training 1 week of training or control time Female
SA25894390430013107Training 1 week of training or control time Male
SA25894490421013107Training 1 week of training or control time Male
SA25894590422013107Training 1 week of training or control time Male
SA25894690423013107Training 1 week of training or control time Male
SA25894790426013107Training 1 week of training or control time Male
SA25894890581013107Training 2 weeks of training Female
SA25894990587013107Training 2 weeks of training Female
SA25895090585013107Training 2 weeks of training Female
SA25895190576013107Training 2 weeks of training Female
SA25895290578013107Training 2 weeks of training Female
SA25895390450013107Training 2 weeks of training Male
SA25895490449013107Training 2 weeks of training Male
SA25895590444013107Training 2 weeks of training Male
SA25895690439013107Training 2 weeks of training Male
SA25895790441013107Training 2 weeks of training Male
SA25895890416013107Training 4 weeks of training Female
SA25895990412013107Training 4 weeks of training Female
SA25896090410013107Training 4 weeks of training Female
SA25896190406013107Training 4 weeks of training Female
SA25896290420013107Training 4 weeks of training Female
SA25896390281013107Training 4 weeks of training Male
SA25896490292013107Training 4 weeks of training Male
SA25896590289013107Training 4 weeks of training Male
SA25896690280013107Training 4 weeks of training Male
SA25896790283013107Training 4 weeks of training Male
SA25896890251013107Training 8 weeks of training or control time Female
SA25896990267013107Training 8 weeks of training or control time Female
SA25897090254013107Training 8 weeks of training or control time Female
SA25897190259013107Training 8 weeks of training or control time Female
SA25897290258013107Training 8 weeks of training or control time Female
SA25897390227013107Training 8 weeks of training or control time Male
SA25897490223013107Training 8 weeks of training or control time Male
SA25897590225013107Training 8 weeks of training or control time Male
SA25897690218013107Training 8 weeks of training or control time Male
SA25897790222013107Training 8 weeks of training or control time Male
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