Summary of Study ST002585

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001665. The data can be accessed directly via it's Project DOI: 10.21228/M8GQ6R This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

Perform statistical analysis  |  Show all samples  |  Show named metabolites  |  Download named metabolite data  
Download mwTab file (text)   |  Download mwTab file(JSON)   |  Download data files (Contains raw data)
Study IDST002585
Study TitleZinc treatment reverses and anti-Zn-regulated miRs suppress esophageal carcinomas in vivo
Study SummaryUsing gas chromatography time-of-flight mass spectrometry GC-TOF MS (3), we performed untargeted metabolomic profiling on esophageal mucosa of Zn-treated vs Zn-untreated rats (n = 10 rats per cohort). Thirty-eight significantly altered metabolites (24 down-, 14 up-regulated, P < 0.05) were identified in the Zn-treated vs Zn-untreated esophagus. Of the 24 down-regulated metabolites, 15 (63%) were involved in anabolic/biosynthetic pathways, including amino acid/pyrimidine/purine metabolism and polyamine biosynthesis. Putrescine (intermediate in polyamine biosynthesis), shown to be up 6.4-fold in ESCC-bearing ZD esophagus (3), was down-regulated -3.96-fold in Zn-treated esophagus. Ornithine decarboxylase (ODC) is the rate liming enzyme in the polyamine biosynthetic pathway to form putrescine, which is converted into spermidine and spermine. Polyamines are indispensable for cell proliferation/tumor growth, and depletion of polyamines inhibits tumor growth (6). Of the 14 metabolites that were significantly up-regulated in the Zn-treated esophagus, five (37%) were carbohydrates, including glucose, which is up-regulated 3.4-fold, pointing to a decreased uptake of glucose and a reversal of the Warburg effect after Zn treatment. Critical to cancer aerobic glycolysis is the metabolic enzyme hexokinase 2 (HK2) that catalyzes the first committed step in glucose metabolism where glucose is phosphorylated to form glucose-6-phosphate. HK2 overexpression accounts for the high glycolytic rate in cancer cells (7). In summary, Zn treatment that significantly reduced ESCC incidence reversed classic cancer cell metabolic phenotypes such as increased glycolysis and nucleoside intermediates, with decrease in putrescine, increase in glucose, accompanied by down-regulation of metabolite enzymes ODC and HK2.
Institute
West Coast Metabolomics Center
Last NamePedrosa
First NameDiego
Address451 Health Science Dr, Davis, CA, 95616, USA
Emaildpedrosa@ucdavis.edu
Phone(530) 752-8129
Submit Date2023-04-20
Raw Data AvailableYes
Raw Data File Type(s)cdf
Analysis Type DetailGC-MS
Release Date2023-05-15
Release Version1
Diego Pedrosa Diego Pedrosa
https://dx.doi.org/10.21228/M8GQ6R
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Factors:

Subject type: Mammal; Subject species: Rattus norvegicus (Factor headings shown in green)

mb_sample_id local_sample_id treatment
SA259171Pool 1Pools
SA259172Pool 2Pools
SA259183A5_005Turmor bearing-untreated
SA259184A4_004Turmor bearing-untreated
SA259185A2_002Turmor bearing-untreated
SA259186A6_006Turmor bearing-untreated
SA259187A3_003Turmor bearing-untreated
SA259188A7_007Turmor bearing-untreated
SA259189A10_010Turmor bearing-untreated
SA259190A9_009Turmor bearing-untreated
SA259191A8_008Turmor bearing-untreated
SA259192A1_001Turmor bearing-untreated
SA259173A16_016Turmor bearing-Zn treated
SA259174A15_015Turmor bearing-Zn treated
SA259175A14_014Turmor bearing-Zn treated
SA259176A17_017Turmor bearing-Zn treated
SA259177A19_019Turmor bearing-Zn treated
SA259178A20_020Turmor bearing-Zn treated
SA259179A13_013Turmor bearing-Zn treated
SA259180A18_018Turmor bearing-Zn treated
SA259181A12_012Turmor bearing-Zn treated
SA259182A11_011Turmor bearing-Zn treated
Showing results 1 to 22 of 22
  logo