Summary of Study ST002595

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001020. The data can be accessed directly via it's Project DOI: 10.21228/M8V97D This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

Study ID	ST002595
Study Title	MoTrPAC: Endurance exercise training study in young adult rats, Tissue:Gastrocnemius - Targeted Nucleotides
Study Summary	The goal of the endurance exercise training study in young adult rats (internal code: PASS1B-06) was to perform exercise training studies in adult (6 month) F344 rats, and from these rats collect as many tissues as feasible in order to provide high quality samples for detailed analysis by chemical analysis sites. Tissues were collected from 10-12 rats sedentary control rats concurrent with the collection of the 8-week training groups. The 8-week training group and controls were from the same cohort and same age at euthanasia (either 8). For the older age group, an additional set of controls (n=5-6) were collected with the 1-2 week training group. Rats were either sedentary or underwent an exercise training program. Rats were exercised on the rodent treadmill 5 days per week using a progressive training protocol designed to exercise the rats at approximately 70% of VO2max as outlined in the Table on the next page. Training was performed no earlier than 10:00 am and no later than 5:00 pm over 5 consecutive days per week. Training was initiated with the treadmill set at 70% of VO2 max (see tables) and 5 degrees grade for 20 minutes. The duration of exercise was increased by one minute each day until day 31 of training (start of week 7), when a duration of 50 min was reached. Speed and grade of each training session increased in larger increments due to treadmill parameters. The highest intensity and duration of training began on day 31. This intensity was maintained for the final 10 days of the protocol to ensure steady state had been achieved. If any rats were unable to perform at least 4 days of training per week they were removed from the study and euthanized. It is important to note that the starting treadmill speed varied depending on the sex and age of the rat. The initial and maximum speeds were based on VO2max measurements obtained during the pre-training testing of the compliant rats. Rats assigned to the control group followed a schedule similar to the training group. They were placed in one lane on the treadmill for 15 minutes/day, 5 days per week. The treadmill was set at 0 m/min at an incline that corresponded to the incline being used by the training group.
Institute	Duke University
Department	Duke Molecular Physiology Institute
Laboratory	Metabolomics Core Laboratory
Last Name	Newgard
First Name	Christopher
Address	300 N Duke St, Durham, NC 27701
Email	chris.newgard@duke.edu
Phone	(919) 668-6059
Submit Date	2023-04-25
Raw Data Available	Yes
Raw Data File Type(s)	TBD
Analysis Type Detail	LC-MS
Release Date	2023-10-06
Release Version	1

Select appropriate tab below to view additional metadata details:

Factors:

Subject type: Mammal; Subject species: Rattus norvegicus (Factor headings shown in green)

mb_sample_id	local_sample_id	Group	Timepoint	Sex
SA259688	90248015514	Control	8 weeks of training or control time	Female
SA259689	90245015514	Control	8 weeks of training or control time	Female
SA259690	90252015514	Control	8 weeks of training or control time	Female
SA259691	90266015514	Control	8 weeks of training or control time	Female
SA259692	90265015514	Control	8 weeks of training or control time	Female
SA259693	90229015514	Control	8 weeks of training or control time	Male
SA259694	90217015514	Control	8 weeks of training or control time	Male
SA259695	90232015514	Control	8 weeks of training or control time	Male
SA259696	90237015514	Control	8 weeks of training or control time	Male
SA259697	90239015514	Control	8 weeks of training or control time	Male
SA259698	80008815502_4	QC-ExternalStandard	0 hour	-
SA259699	80008815502_2	QC-ExternalStandard	0 hour	-
SA259700	80009825502_2	QC-ExternalStandard	0 hour	-
SA259701	80009825502_4	QC-ExternalStandard	0 hour	-
SA259702	80009825502_1	QC-ExternalStandard	0 hour	-
SA259703	80008815502_3	QC-ExternalStandard	0 hour	-
SA259704	80009825502_3	QC-ExternalStandard	0 hour	-
SA259705	80008815502_1	QC-ExternalStandard	0 hour	-
SA259706	90564015514	Training	1 week of training or control time	Female
SA259707	90559015514	Training	1 week of training or control time	Female
SA259708	90567015514	Training	1 week of training or control time	Female
SA259709	90560015514	Training	1 week of training or control time	Female
SA259710	90571015514	Training	1 week of training or control time	Female
SA259711	90422015514	Training	1 week of training or control time	Male
SA259712	90426015514	Training	1 week of training or control time	Male
SA259713	90423015514	Training	1 week of training or control time	Male
SA259714	90421015514	Training	1 week of training or control time	Male
SA259715	90430015514	Training	1 week of training or control time	Male
SA259716	90578015514	Training	2 weeks of training	Female
SA259717	90576015514	Training	2 weeks of training	Female
SA259718	90581015514	Training	2 weeks of training	Female
SA259719	90587015514	Training	2 weeks of training	Female
SA259720	90585015514	Training	2 weeks of training	Female
SA259721	90441015514	Training	2 weeks of training	Male
SA259722	90444015514	Training	2 weeks of training	Male
SA259723	90450015514	Training	2 weeks of training	Male
SA259724	90449015514	Training	2 weeks of training	Male
SA259725	90439015514	Training	2 weeks of training	Male
SA259726	90410015514	Training	4 weeks of training	Female
SA259727	90416015514	Training	4 weeks of training	Female
SA259728	90420015514	Training	4 weeks of training	Female
SA259729	90406015514	Training	4 weeks of training	Female
SA259730	90412015514	Training	4 weeks of training	Female
SA259731	90280015514	Training	4 weeks of training	Male
SA259732	90289015514	Training	4 weeks of training	Male
SA259733	90283015514	Training	4 weeks of training	Male
SA259734	90281015514	Training	4 weeks of training	Male
SA259735	90292015514	Training	4 weeks of training	Male
SA259736	90258015514	Training	8 weeks of training or control time	Female
SA259737	90259015514	Training	8 weeks of training or control time	Female
SA259738	90254015514	Training	8 weeks of training or control time	Female
SA259739	90267015514	Training	8 weeks of training or control time	Female
SA259740	90251015514	Training	8 weeks of training or control time	Female
SA259741	90223015514	Training	8 weeks of training or control time	Male
SA259742	90227015514	Training	8 weeks of training or control time	Male
SA259743	90218015514	Training	8 weeks of training or control time	Male
SA259744	90222015514	Training	8 weeks of training or control time	Male
SA259745	90225015514	Training	8 weeks of training or control time	Male

Showing results 1 to 58 of 58

Showing results 1 to 58 of 58