Summary of Study ST002634

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001020. The data can be accessed directly via it's Project DOI: 10.21228/M8V97D This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

Study ID	ST002634
Study Title	MoTrPAC: Endurance exercise training study in young adult rats, Tissue:Hippocampus - Untargeted HILIC-Positive
Study Summary	The goal of the endurance exercise training study in young adult rats (internal code: PASS1B-06) was to perform exercise training studies in adult (6 month) F344 rats, and from these rats collect as many tissues as feasible in order to provide high quality samples for detailed analysis by chemical analysis sites. Tissues were collected from 10-12 rats sedentary control rats concurrent with the collection of the 8-week training groups. The 8-week training group and controls were from the same cohort and same age at euthanasia (either 8). For the older age group, an additional set of controls (n=5-6) were collected with the 1-2 week training group. Rats were either sedentary or underwent an exercise training program. Rats were exercised on the rodent treadmill 5 days per week using a progressive training protocol designed to exercise the rats at approximately 70% of VO2max as outlined in the Table on the next page. Training was performed no earlier than 10:00 am and no later than 5:00 pm over 5 consecutive days per week. Training was initiated with the treadmill set at 70% of VO2 max (see tables) and 5 degrees grade for 20 minutes. The duration of exercise was increased by one minute each day until day 31 of training (start of week 7), when a duration of 50 min was reached. Speed and grade of each training session increased in larger increments due to treadmill parameters. The highest intensity and duration of training began on day 31. This intensity was maintained for the final 10 days of the protocol to ensure steady state had been achieved. If any rats were unable to perform at least 4 days of training per week they were removed from the study and euthanized. It is important to note that the starting treadmill speed varied depending on the sex and age of the rat. The initial and maximum speeds were based on VO2max measurements obtained during the pre-training testing of the compliant rats. Rats assigned to the control group followed a schedule similar to the training group. They were placed in one lane on the treadmill for 15 minutes/day, 5 days per week. The treadmill was set at 0 m/min at an incline that corresponded to the incline being used by the training group.
Institute	Broad Institute of MIT and Harvard
Department	Metabolomics Platform
Laboratory	Clish
Last Name	Clish
First Name	Clary
Address	415 Main St, Cambridge, MA 02142
Email	clary@broadinstitute.org
Phone	(617) 714-7654
Submit Date	2023-05-01
Raw Data Available	Yes
Raw Data File Type(s)	TBD
Analysis Type Detail	LC-MS
Release Date	2023-10-06
Release Version	1

Select appropriate tab below to view additional metadata details:

Factors:

Subject type: Mammal; Subject species: Rattus norvegicus (Factor headings shown in green)

mb_sample_id	local_sample_id	Group	Timepoint	Sex
SA262139	90245015205	Control	8 weeks of training or control time	Female
SA262140	90248015205	Control	8 weeks of training or control time	Female
SA262141	90252015205	Control	8 weeks of training or control time	Female
SA262142	90266015205	Control	8 weeks of training or control time	Female
SA262143	90265015205	Control	8 weeks of training or control time	Female
SA262144	90237015205	Control	8 weeks of training or control time	Male
SA262145	90229015205	Control	8 weeks of training or control time	Male
SA262146	90239015205	Control	8 weeks of training or control time	Male
SA262147	90217015205	Control	8 weeks of training or control time	Male
SA262148	90232015205	Control	8 weeks of training or control time	Male
SA262149	QC_PREFA03	QC-DriftCorrection	0 hour	-
SA262150	QC_PREFA01	QC-DriftCorrection	0 hour	-
SA262151	QC_PREFA02	QC-DriftCorrection	0 hour	-
SA262152	QC_PREFA04	QC-DriftCorrection	0 hour	-
SA262153	QC_PREFB02	QC-Pooled	0 hour	-
SA262154	QC_PREFB04	QC-Pooled	0 hour	-
SA262155	QC_PREFB01	QC-Pooled	0 hour	-
SA262156	QC_PREFB03	QC-Pooled	0 hour	-
SA262157	QC_Sed02	QC-Reference	0 hour	-
SA262158	QC_IPE01	QC-Reference	0 hour	-
SA262159	QC_IPE04	QC-Reference	0 hour	-
SA262160	QC_IPE03	QC-Reference	0 hour	-
SA262161	QC_Sed01	QC-Reference	0 hour	-
SA262162	QC_Sed04	QC-Reference	0 hour	-
SA262163	QC_IPE02	QC-Reference	0 hour	-
SA262164	QC_Sed03	QC-Reference	0 hour	-
SA262165	90564015205	Training	1 week of training or control time	Female
SA262166	90567015205	Training	1 week of training or control time	Female
SA262167	90559015205	Training	1 week of training or control time	Female
SA262168	90560015205	Training	1 week of training or control time	Female
SA262169	90571015205	Training	1 week of training or control time	Female
SA262170	90423015205	Training	1 week of training or control time	Male
SA262171	90426015205	Training	1 week of training or control time	Male
SA262172	90430015205	Training	1 week of training or control time	Male
SA262173	90421015205	Training	1 week of training or control time	Male
SA262174	90422015205	Training	1 week of training or control time	Male
SA262175	90578015205	Training	2 weeks of training	Female
SA262176	90587015205	Training	2 weeks of training	Female
SA262177	90581015205	Training	2 weeks of training	Female
SA262178	90585015205	Training	2 weeks of training	Female
SA262179	90576015205	Training	2 weeks of training	Female
SA262180	90449015205	Training	2 weeks of training	Male
SA262181	90444015205	Training	2 weeks of training	Male
SA262182	90439015205	Training	2 weeks of training	Male
SA262183	90450015205	Training	2 weeks of training	Male
SA262184	90441015205	Training	2 weeks of training	Male
SA262185	90406015205	Training	4 weeks of training	Female
SA262186	90420015205	Training	4 weeks of training	Female
SA262187	90410015205	Training	4 weeks of training	Female
SA262188	90412015205	Training	4 weeks of training	Female
SA262189	90416015205	Training	4 weeks of training	Female
SA262190	90280015205	Training	4 weeks of training	Male
SA262191	90281015205	Training	4 weeks of training	Male
SA262192	90289015205	Training	4 weeks of training	Male
SA262193	90292015205	Training	4 weeks of training	Male
SA262194	90283015205	Training	4 weeks of training	Male
SA262195	90251015205	Training	8 weeks of training or control time	Female
SA262196	90254015205	Training	8 weeks of training or control time	Female
SA262197	90267015205	Training	8 weeks of training or control time	Female
SA262198	90258015205	Training	8 weeks of training or control time	Female
SA262199	90259015205	Training	8 weeks of training or control time	Female
SA262200	90222015205	Training	8 weeks of training or control time	Male
SA262201	90218015205	Training	8 weeks of training or control time	Male
SA262202	90225015205	Training	8 weeks of training or control time	Male
SA262203	90227015205	Training	8 weeks of training or control time	Male
SA262204	90223015205	Training	8 weeks of training or control time	Male

Showing results 1 to 66 of 66

Showing results 1 to 66 of 66