Summary of Study ST002640

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001020. The data can be accessed directly via it's Project DOI: 10.21228/M8V97D This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

Study ID	ST002640
Study Title	MoTrPAC: Endurance exercise training study in young adult rats, Tissue:Cortex - Untargeted HILIC-Positive
Study Summary	The goal of the endurance exercise training study in young adult rats (internal code: PASS1B-06) was to perform exercise training studies in adult (6 month) F344 rats, and from these rats collect as many tissues as feasible in order to provide high quality samples for detailed analysis by chemical analysis sites. Tissues were collected from 10-12 rats sedentary control rats concurrent with the collection of the 8-week training groups. The 8-week training group and controls were from the same cohort and same age at euthanasia (either 8). For the older age group, an additional set of controls (n=5-6) were collected with the 1-2 week training group. Rats were either sedentary or underwent an exercise training program. Rats were exercised on the rodent treadmill 5 days per week using a progressive training protocol designed to exercise the rats at approximately 70% of VO2max as outlined in the Table on the next page. Training was performed no earlier than 10:00 am and no later than 5:00 pm over 5 consecutive days per week. Training was initiated with the treadmill set at 70% of VO2 max (see tables) and 5 degrees grade for 20 minutes. The duration of exercise was increased by one minute each day until day 31 of training (start of week 7), when a duration of 50 min was reached. Speed and grade of each training session increased in larger increments due to treadmill parameters. The highest intensity and duration of training began on day 31. This intensity was maintained for the final 10 days of the protocol to ensure steady state had been achieved. If any rats were unable to perform at least 4 days of training per week they were removed from the study and euthanized. It is important to note that the starting treadmill speed varied depending on the sex and age of the rat. The initial and maximum speeds were based on VO2max measurements obtained during the pre-training testing of the compliant rats. Rats assigned to the control group followed a schedule similar to the training group. They were placed in one lane on the treadmill for 15 minutes/day, 5 days per week. The treadmill was set at 0 m/min at an incline that corresponded to the incline being used by the training group.
Institute	Broad Institute of MIT and Harvard
Department	Metabolomics Platform
Laboratory	Clish
Last Name	Clish
First Name	Clary
Address	415 Main St, Cambridge, MA 02142
Email	clary@broadinstitute.org
Phone	(617) 714-7654
Submit Date	2023-05-01
Raw Data Available	Yes
Raw Data File Type(s)	TBD
Analysis Type Detail	LC-MS
Release Date	2023-10-06
Release Version	1

Select appropriate tab below to view additional metadata details:

Factors:

Subject type: Mammal; Subject species: Rattus norvegicus (Factor headings shown in green)

mb_sample_id	local_sample_id	Group	Timepoint	Sex
SA262608	90245015303	Control	8 weeks of training or control time	Female
SA262609	90248015303	Control	8 weeks of training or control time	Female
SA262610	90252015303	Control	8 weeks of training or control time	Female
SA262611	90266015303	Control	8 weeks of training or control time	Female
SA262612	90265015303	Control	8 weeks of training or control time	Female
SA262613	90237015303	Control	8 weeks of training or control time	Male
SA262614	90229015303	Control	8 weeks of training or control time	Male
SA262615	90239015303	Control	8 weeks of training or control time	Male
SA262616	90217015303	Control	8 weeks of training or control time	Male
SA262617	90232015303	Control	8 weeks of training or control time	Male
SA262618	QC_PREFA03	QC-DriftCorrection	0 hour	-
SA262619	QC_PREFA01	QC-DriftCorrection	0 hour	-
SA262620	QC_PREFA02	QC-DriftCorrection	0 hour	-
SA262621	QC_PREFA04	QC-DriftCorrection	0 hour	-
SA262622	QC_PREFB02	QC-Pooled	0 hour	-
SA262623	QC_PREFB04	QC-Pooled	0 hour	-
SA262624	QC_PREFB01	QC-Pooled	0 hour	-
SA262625	QC_PREFB03	QC-Pooled	0 hour	-
SA262626	QC_Sed02	QC-Reference	0 hour	-
SA262627	QC_IPE01	QC-Reference	0 hour	-
SA262628	QC_IPE04	QC-Reference	0 hour	-
SA262629	QC_IPE03	QC-Reference	0 hour	-
SA262630	QC_Sed01	QC-Reference	0 hour	-
SA262631	QC_Sed04	QC-Reference	0 hour	-
SA262632	QC_IPE02	QC-Reference	0 hour	-
SA262633	QC_Sed03	QC-Reference	0 hour	-
SA262634	90564015303	Training	1 week of training or control time	Female
SA262635	90567015303	Training	1 week of training or control time	Female
SA262636	90559015303	Training	1 week of training or control time	Female
SA262637	90560015303	Training	1 week of training or control time	Female
SA262638	90571015303	Training	1 week of training or control time	Female
SA262639	90423015303	Training	1 week of training or control time	Male
SA262640	90426015303	Training	1 week of training or control time	Male
SA262641	90430015303	Training	1 week of training or control time	Male
SA262642	90421015303	Training	1 week of training or control time	Male
SA262643	90422015303	Training	1 week of training or control time	Male
SA262644	90578015303	Training	2 weeks of training	Female
SA262645	90587015303	Training	2 weeks of training	Female
SA262646	90581015303	Training	2 weeks of training	Female
SA262647	90585015303	Training	2 weeks of training	Female
SA262648	90576015303	Training	2 weeks of training	Female
SA262649	90449015303	Training	2 weeks of training	Male
SA262650	90444015303	Training	2 weeks of training	Male
SA262651	90439015303	Training	2 weeks of training	Male
SA262652	90450015303	Training	2 weeks of training	Male
SA262653	90441015303	Training	2 weeks of training	Male
SA262654	90406015303	Training	4 weeks of training	Female
SA262655	90420015303	Training	4 weeks of training	Female
SA262656	90410015303	Training	4 weeks of training	Female
SA262657	90412015303	Training	4 weeks of training	Female
SA262658	90416015303	Training	4 weeks of training	Female
SA262659	90280015303	Training	4 weeks of training	Male
SA262660	90281015303	Training	4 weeks of training	Male
SA262661	90289015303	Training	4 weeks of training	Male
SA262662	90292015303	Training	4 weeks of training	Male
SA262663	90283015303	Training	4 weeks of training	Male
SA262664	90251015303	Training	8 weeks of training or control time	Female
SA262665	90254015303	Training	8 weeks of training or control time	Female
SA262666	90267015303	Training	8 weeks of training or control time	Female
SA262667	90258015303	Training	8 weeks of training or control time	Female
SA262668	90259015303	Training	8 weeks of training or control time	Female
SA262669	90222015303	Training	8 weeks of training or control time	Male
SA262670	90218015303	Training	8 weeks of training or control time	Male
SA262671	90225015303	Training	8 weeks of training or control time	Male
SA262672	90227015303	Training	8 weeks of training or control time	Male
SA262673	90223015303	Training	8 weeks of training or control time	Male

Showing results 1 to 66 of 66

Showing results 1 to 66 of 66