Summary of Study ST002641

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001020. The data can be accessed directly via it's Project DOI: 10.21228/M8V97D This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

Study ID	ST002641
Study Title	MoTrPAC: Endurance exercise training study in young adult rats, Tissue:Hypothalamus - Untargeted HILIC-Positive
Study Summary	The goal of the endurance exercise training study in young adult rats (internal code: PASS1B-06) was to perform exercise training studies in adult (6 month) F344 rats, and from these rats collect as many tissues as feasible in order to provide high quality samples for detailed analysis by chemical analysis sites. Tissues were collected from 10-12 rats sedentary control rats concurrent with the collection of the 8-week training groups. The 8-week training group and controls were from the same cohort and same age at euthanasia (either 8). For the older age group, an additional set of controls (n=5-6) were collected with the 1-2 week training group. Rats were either sedentary or underwent an exercise training program. Rats were exercised on the rodent treadmill 5 days per week using a progressive training protocol designed to exercise the rats at approximately 70% of VO2max as outlined in the Table on the next page. Training was performed no earlier than 10:00 am and no later than 5:00 pm over 5 consecutive days per week. Training was initiated with the treadmill set at 70% of VO2 max (see tables) and 5 degrees grade for 20 minutes. The duration of exercise was increased by one minute each day until day 31 of training (start of week 7), when a duration of 50 min was reached. Speed and grade of each training session increased in larger increments due to treadmill parameters. The highest intensity and duration of training began on day 31. This intensity was maintained for the final 10 days of the protocol to ensure steady state had been achieved. If any rats were unable to perform at least 4 days of training per week they were removed from the study and euthanized. It is important to note that the starting treadmill speed varied depending on the sex and age of the rat. The initial and maximum speeds were based on VO2max measurements obtained during the pre-training testing of the compliant rats. Rats assigned to the control group followed a schedule similar to the training group. They were placed in one lane on the treadmill for 15 minutes/day, 5 days per week. The treadmill was set at 0 m/min at an incline that corresponded to the incline being used by the training group.
Institute	Broad Institute of MIT and Harvard
Department	Metabolomics Platform
Laboratory	Clish
Last Name	Clish
First Name	Clary
Address	415 Main St, Cambridge, MA 02142
Email	clary@broadinstitute.org
Phone	(617) 714-7654
Submit Date	2023-05-01
Raw Data Available	Yes
Raw Data File Type(s)	TBD
Analysis Type Detail	LC-MS
Release Date	2023-10-06
Release Version	1

Select appropriate tab below to view additional metadata details:

Factors:

Subject type: Mammal; Subject species: Rattus norvegicus (Factor headings shown in green)

mb_sample_id	local_sample_id	Group	Timepoint	Sex
SA262674	90248015403	Control	8 weeks of training or control time	Female
SA262675	90245015403	Control	8 weeks of training or control time	Female
SA262676	90252015403	Control	8 weeks of training or control time	Female
SA262677	90265015403	Control	8 weeks of training or control time	Female
SA262678	90266015403	Control	8 weeks of training or control time	Female
SA262679	90237015403	Control	8 weeks of training or control time	Male
SA262680	90232015403	Control	8 weeks of training or control time	Male
SA262681	90229015403	Control	8 weeks of training or control time	Male
SA262682	QC_PREFA02	QC-DriftCorrection	0 hour	-
SA262683	QC_PREFA04	QC-DriftCorrection	0 hour	-
SA262684	QC_PREFA03	QC-DriftCorrection	0 hour	-
SA262685	QC_PREFA01	QC-DriftCorrection	0 hour	-
SA262686	QC_PREFB01	QC-Pooled	0 hour	-
SA262687	QC_PREFB03	QC-Pooled	0 hour	-
SA262688	QC_PREFB04	QC-Pooled	0 hour	-
SA262689	QC_PREFB02	QC-Pooled	0 hour	-
SA262690	QC_Sed03	QC-Reference	0 hour	-
SA262691	QC_Sed04	QC-Reference	0 hour	-
SA262692	QC_IPE01	QC-Reference	0 hour	-
SA262693	QC_Sed02	QC-Reference	0 hour	-
SA262694	QC_Sed01	QC-Reference	0 hour	-
SA262695	QC_IPE02	QC-Reference	0 hour	-
SA262696	QC_IPE04	QC-Reference	0 hour	-
SA262697	QC_IPE03	QC-Reference	0 hour	-
SA262698	90567015403	Training	1 week of training or control time	Female
SA262699	90564015403	Training	1 week of training or control time	Female
SA262700	90559015403	Training	1 week of training or control time	Female
SA262701	90560015403	Training	1 week of training or control time	Female
SA262702	90571015403	Training	1 week of training or control time	Female
SA262703	90421015403	Training	1 week of training or control time	Male
SA262704	90423015403	Training	1 week of training or control time	Male
SA262705	90430015403	Training	1 week of training or control time	Male
SA262706	90426015403	Training	1 week of training or control time	Male
SA262707	90422015403	Training	1 week of training or control time	Male
SA262708	90585015403	Training	2 weeks of training	Female
SA262709	90576015403	Training	2 weeks of training	Female
SA262710	90587015403	Training	2 weeks of training	Female
SA262711	90581015403	Training	2 weeks of training	Female
SA262712	90578015403	Training	2 weeks of training	Female
SA262713	90450015403	Training	2 weeks of training	Male
SA262714	90449015403	Training	2 weeks of training	Male
SA262715	90441015403	Training	2 weeks of training	Male
SA262716	90439015403	Training	2 weeks of training	Male
SA262717	90444015403	Training	2 weeks of training	Male
SA262718	90412015403	Training	4 weeks of training	Female
SA262719	90420015403	Training	4 weeks of training	Female
SA262720	90406015403	Training	4 weeks of training	Female
SA262721	90410015403	Training	4 weeks of training	Female
SA262722	90416015403	Training	4 weeks of training	Female
SA262723	90281015403	Training	4 weeks of training	Male
SA262724	90283015403	Training	4 weeks of training	Male
SA262725	90280015403	Training	4 weeks of training	Male
SA262726	90289015403	Training	4 weeks of training	Male
SA262727	90292015403	Training	4 weeks of training	Male
SA262728	90254015403	Training	8 weeks of training or control time	Female
SA262729	90251015403	Training	8 weeks of training or control time	Female
SA262730	90258015403	Training	8 weeks of training or control time	Female
SA262731	90259015403	Training	8 weeks of training or control time	Female
SA262732	90267015403	Training	8 weeks of training or control time	Female
SA262733	90218015403	Training	8 weeks of training or control time	Male
SA262734	90227015403	Training	8 weeks of training or control time	Male
SA262735	90225015403	Training	8 weeks of training or control time	Male

Showing results 1 to 62 of 62

Showing results 1 to 62 of 62