Summary of Study ST002648

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001020. The data can be accessed directly via it's Project DOI: 10.21228/M8V97D This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

Study ID	ST002648
Study Title	MoTrPAC: Endurance exercise training study in young adult rats, Tissue:Vastus Lateralis - Untargeted HILIC-Positive
Study Summary	The goal of the endurance exercise training study in young adult rats (internal code: PASS1B-06) was to perform exercise training studies in adult (6 month) F344 rats, and from these rats collect as many tissues as feasible in order to provide high quality samples for detailed analysis by chemical analysis sites. Tissues were collected from 10-12 rats sedentary control rats concurrent with the collection of the 8-week training groups. The 8-week training group and controls were from the same cohort and same age at euthanasia (either 8). For the older age group, an additional set of controls (n=5-6) were collected with the 1-2 week training group. Rats were either sedentary or underwent an exercise training program. Rats were exercised on the rodent treadmill 5 days per week using a progressive training protocol designed to exercise the rats at approximately 70% of VO2max as outlined in the Table on the next page. Training was performed no earlier than 10:00 am and no later than 5:00 pm over 5 consecutive days per week. Training was initiated with the treadmill set at 70% of VO2 max (see tables) and 5 degrees grade for 20 minutes. The duration of exercise was increased by one minute each day until day 31 of training (start of week 7), when a duration of 50 min was reached. Speed and grade of each training session increased in larger increments due to treadmill parameters. The highest intensity and duration of training began on day 31. This intensity was maintained for the final 10 days of the protocol to ensure steady state had been achieved. If any rats were unable to perform at least 4 days of training per week they were removed from the study and euthanized. It is important to note that the starting treadmill speed varied depending on the sex and age of the rat. The initial and maximum speeds were based on VO2max measurements obtained during the pre-training testing of the compliant rats. Rats assigned to the control group followed a schedule similar to the training group. They were placed in one lane on the treadmill for 15 minutes/day, 5 days per week. The treadmill was set at 0 m/min at an incline that corresponded to the incline being used by the training group.
Institute	Broad Institute of MIT and Harvard
Department	Metabolomics Platform
Laboratory	Clish
Last Name	Clish
First Name	Clary
Address	415 Main St, Cambridge, MA 02142
Email	clary@broadinstitute.org
Phone	(617) 714-7654
Submit Date	2023-05-01
Raw Data Available	Yes
Raw Data File Type(s)	TBD
Analysis Type Detail	LC-MS
Release Date	2023-10-06
Release Version	1

Select appropriate tab below to view additional metadata details:

Factors:

Subject type: Mammal; Subject species: Rattus norvegicus (Factor headings shown in green)

mb_sample_id	local_sample_id	Group	Timepoint	Sex
SA263205	90245015604	Control	8 weeks of training or control time	Female
SA263206	90248015604	Control	8 weeks of training or control time	Female
SA263207	90252015604	Control	8 weeks of training or control time	Female
SA263208	90266015604	Control	8 weeks of training or control time	Female
SA263209	90265015604	Control	8 weeks of training or control time	Female
SA263210	90237015604	Control	8 weeks of training or control time	Male
SA263211	90229015604	Control	8 weeks of training or control time	Male
SA263212	90239015604	Control	8 weeks of training or control time	Male
SA263213	90217015604	Control	8 weeks of training or control time	Male
SA263214	90232015604	Control	8 weeks of training or control time	Male
SA263215	QC_PREFA03	QC-DriftCorrection	0 hour	-
SA263216	QC_PREFA01	QC-DriftCorrection	0 hour	-
SA263217	QC_PREFA02	QC-DriftCorrection	0 hour	-
SA263218	QC_PREFA04	QC-DriftCorrection	0 hour	-
SA263219	QC_PREFB02	QC-Pooled	0 hour	-
SA263220	QC_PREFB04	QC-Pooled	0 hour	-
SA263221	QC_PREFB01	QC-Pooled	0 hour	-
SA263222	QC_PREFB03	QC-Pooled	0 hour	-
SA263223	QC_Sed02	QC-Reference	0 hour	-
SA263224	QC_IPE01	QC-Reference	0 hour	-
SA263225	QC_IPE04	QC-Reference	0 hour	-
SA263226	QC_IPE03	QC-Reference	0 hour	-
SA263227	QC_Sed01	QC-Reference	0 hour	-
SA263228	QC_Sed04	QC-Reference	0 hour	-
SA263229	QC_IPE02	QC-Reference	0 hour	-
SA263230	QC_Sed03	QC-Reference	0 hour	-
SA263231	90564015604	Training	1 week of training or control time	Female
SA263232	90567015604	Training	1 week of training or control time	Female
SA263233	90559015604	Training	1 week of training or control time	Female
SA263234	90560015604	Training	1 week of training or control time	Female
SA263235	90571015604	Training	1 week of training or control time	Female
SA263236	90423015604	Training	1 week of training or control time	Male
SA263237	90426015604	Training	1 week of training or control time	Male
SA263238	90430015604	Training	1 week of training or control time	Male
SA263239	90421015604	Training	1 week of training or control time	Male
SA263240	90422015604	Training	1 week of training or control time	Male
SA263241	90578015604	Training	2 weeks of training	Female
SA263242	90587015604	Training	2 weeks of training	Female
SA263243	90581015604	Training	2 weeks of training	Female
SA263244	90585015604	Training	2 weeks of training	Female
SA263245	90576015604	Training	2 weeks of training	Female
SA263246	90449015604	Training	2 weeks of training	Male
SA263247	90444015604	Training	2 weeks of training	Male
SA263248	90439015604	Training	2 weeks of training	Male
SA263249	90450015604	Training	2 weeks of training	Male
SA263250	90441015604	Training	2 weeks of training	Male
SA263251	90406015604	Training	4 weeks of training	Female
SA263252	90420015604	Training	4 weeks of training	Female
SA263253	90410015604	Training	4 weeks of training	Female
SA263254	90412015604	Training	4 weeks of training	Female
SA263255	90416015604	Training	4 weeks of training	Female
SA263256	90280015604	Training	4 weeks of training	Male
SA263257	90281015604	Training	4 weeks of training	Male
SA263258	90289015604	Training	4 weeks of training	Male
SA263259	90292015604	Training	4 weeks of training	Male
SA263260	90283015604	Training	4 weeks of training	Male
SA263261	90251015604	Training	8 weeks of training or control time	Female
SA263262	90254015604	Training	8 weeks of training or control time	Female
SA263263	90267015604	Training	8 weeks of training or control time	Female
SA263264	90258015604	Training	8 weeks of training or control time	Female
SA263265	90259015604	Training	8 weeks of training or control time	Female
SA263266	90222015604	Training	8 weeks of training or control time	Male
SA263267	90218015604	Training	8 weeks of training or control time	Male
SA263268	90225015604	Training	8 weeks of training or control time	Male
SA263269	90227015604	Training	8 weeks of training or control time	Male
SA263270	90223015604	Training	8 weeks of training or control time	Male

Showing results 1 to 66 of 66

Showing results 1 to 66 of 66