Summary of Study ST002649

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001020. The data can be accessed directly via it's Project DOI: 10.21228/M8V97D This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

Study ID	ST002649
Study Title	MoTrPAC: Endurance exercise training study in young adult rats, Tissue:Heart - Untargeted HILIC-Positive
Study Summary	The goal of the endurance exercise training study in young adult rats (internal code: PASS1B-06) was to perform exercise training studies in adult (6 month) F344 rats, and from these rats collect as many tissues as feasible in order to provide high quality samples for detailed analysis by chemical analysis sites. Tissues were collected from 10-12 rats sedentary control rats concurrent with the collection of the 8-week training groups. The 8-week training group and controls were from the same cohort and same age at euthanasia (either 8). For the older age group, an additional set of controls (n=5-6) were collected with the 1-2 week training group. Rats were either sedentary or underwent an exercise training program. Rats were exercised on the rodent treadmill 5 days per week using a progressive training protocol designed to exercise the rats at approximately 70% of VO2max as outlined in the Table on the next page. Training was performed no earlier than 10:00 am and no later than 5:00 pm over 5 consecutive days per week. Training was initiated with the treadmill set at 70% of VO2 max (see tables) and 5 degrees grade for 20 minutes. The duration of exercise was increased by one minute each day until day 31 of training (start of week 7), when a duration of 50 min was reached. Speed and grade of each training session increased in larger increments due to treadmill parameters. The highest intensity and duration of training began on day 31. This intensity was maintained for the final 10 days of the protocol to ensure steady state had been achieved. If any rats were unable to perform at least 4 days of training per week they were removed from the study and euthanized. It is important to note that the starting treadmill speed varied depending on the sex and age of the rat. The initial and maximum speeds were based on VO2max measurements obtained during the pre-training testing of the compliant rats. Rats assigned to the control group followed a schedule similar to the training group. They were placed in one lane on the treadmill for 15 minutes/day, 5 days per week. The treadmill was set at 0 m/min at an incline that corresponded to the incline being used by the training group.
Institute	Broad Institute of MIT and Harvard
Department	Metabolomics Platform
Laboratory	Clish
Last Name	Clish
First Name	Clary
Address	415 Main St, Cambridge, MA 02142
Email	clary@broadinstitute.org
Phone	(617) 714-7654
Submit Date	2023-05-01
Raw Data Available	Yes
Raw Data File Type(s)	TBD
Analysis Type Detail	LC-MS
Release Date	2023-10-06
Release Version	1

Select appropriate tab below to view additional metadata details:

Factors:

Subject type: Mammal; Subject species: Rattus norvegicus (Factor headings shown in green)

mb_sample_id	local_sample_id	Group	Timepoint	Sex
SA263271	90245015806	Control	8 weeks of training or control time	Female
SA263272	90248015806	Control	8 weeks of training or control time	Female
SA263273	90252015806	Control	8 weeks of training or control time	Female
SA263274	90266015806	Control	8 weeks of training or control time	Female
SA263275	90265015806	Control	8 weeks of training or control time	Female
SA263276	90237015806	Control	8 weeks of training or control time	Male
SA263277	90229015806	Control	8 weeks of training or control time	Male
SA263278	90239015806	Control	8 weeks of training or control time	Male
SA263279	90217015806	Control	8 weeks of training or control time	Male
SA263280	90232015806	Control	8 weeks of training or control time	Male
SA263281	QC_PREFA03	QC-DriftCorrection	0 hour	-
SA263282	QC_PREFA01	QC-DriftCorrection	0 hour	-
SA263283	QC_PREFA02	QC-DriftCorrection	0 hour	-
SA263284	QC_PREFA04	QC-DriftCorrection	0 hour	-
SA263285	QC_PREFB02	QC-Pooled	0 hour	-
SA263286	QC_PREFB04	QC-Pooled	0 hour	-
SA263287	QC_PREFB01	QC-Pooled	0 hour	-
SA263288	QC_PREFB03	QC-Pooled	0 hour	-
SA263289	QC_Sed02	QC-Reference	0 hour	-
SA263290	QC_IPE01	QC-Reference	0 hour	-
SA263291	QC_IPE04	QC-Reference	0 hour	-
SA263292	QC_IPE03	QC-Reference	0 hour	-
SA263293	QC_Sed01	QC-Reference	0 hour	-
SA263294	QC_Sed04	QC-Reference	0 hour	-
SA263295	QC_IPE02	QC-Reference	0 hour	-
SA263296	QC_Sed03	QC-Reference	0 hour	-
SA263297	90564015806	Training	1 week of training or control time	Female
SA263298	90567015806	Training	1 week of training or control time	Female
SA263299	90559015806	Training	1 week of training or control time	Female
SA263300	90560015806	Training	1 week of training or control time	Female
SA263301	90571015806	Training	1 week of training or control time	Female
SA263302	90423015806	Training	1 week of training or control time	Male
SA263303	90426015806	Training	1 week of training or control time	Male
SA263304	90430015806	Training	1 week of training or control time	Male
SA263305	90421015806	Training	1 week of training or control time	Male
SA263306	90422015806	Training	1 week of training or control time	Male
SA263307	90578015806	Training	2 weeks of training	Female
SA263308	90587015806	Training	2 weeks of training	Female
SA263309	90581015806	Training	2 weeks of training	Female
SA263310	90585015806	Training	2 weeks of training	Female
SA263311	90576015806	Training	2 weeks of training	Female
SA263312	90449015806	Training	2 weeks of training	Male
SA263313	90444015806	Training	2 weeks of training	Male
SA263314	90439015806	Training	2 weeks of training	Male
SA263315	90450015806	Training	2 weeks of training	Male
SA263316	90441015806	Training	2 weeks of training	Male
SA263317	90406015806	Training	4 weeks of training	Female
SA263318	90420015806	Training	4 weeks of training	Female
SA263319	90410015806	Training	4 weeks of training	Female
SA263320	90412015806	Training	4 weeks of training	Female
SA263321	90416015806	Training	4 weeks of training	Female
SA263322	90280015806	Training	4 weeks of training	Male
SA263323	90281015806	Training	4 weeks of training	Male
SA263324	90289015806	Training	4 weeks of training	Male
SA263325	90292015806	Training	4 weeks of training	Male
SA263326	90283015806	Training	4 weeks of training	Male
SA263327	90251015806	Training	8 weeks of training or control time	Female
SA263328	90254015806	Training	8 weeks of training or control time	Female
SA263329	90267015806	Training	8 weeks of training or control time	Female
SA263330	90258015806	Training	8 weeks of training or control time	Female
SA263331	90259015806	Training	8 weeks of training or control time	Female
SA263332	90222015806	Training	8 weeks of training or control time	Male
SA263333	90218015806	Training	8 weeks of training or control time	Male
SA263334	90225015806	Training	8 weeks of training or control time	Male
SA263335	90227015806	Training	8 weeks of training or control time	Male
SA263336	90223015806	Training	8 weeks of training or control time	Male

Showing results 1 to 66 of 66

Showing results 1 to 66 of 66