Summary of Study ST002659

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001020. The data can be accessed directly via it's Project DOI: 10.21228/M8V97D This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

Study ID	ST002659
Study Title	MoTrPAC: Endurance exercise training study in young adult rats, Tissue:Adrenal - Untargeted HILIC-Positive
Study Summary	The goal of the endurance exercise training study in young adult rats (internal code: PASS1B-06) was to perform exercise training studies in adult (6 month) F344 rats, and from these rats collect as many tissues as feasible in order to provide high quality samples for detailed analysis by chemical analysis sites. Tissues were collected from 10-12 rats sedentary control rats concurrent with the collection of the 8-week training groups. The 8-week training group and controls were from the same cohort and same age at euthanasia (either 8). For the older age group, an additional set of controls (n=5-6) were collected with the 1-2 week training group. Rats were either sedentary or underwent an exercise training program. Rats were exercised on the rodent treadmill 5 days per week using a progressive training protocol designed to exercise the rats at approximately 70% of VO2max as outlined in the Table on the next page. Training was performed no earlier than 10:00 am and no later than 5:00 pm over 5 consecutive days per week. Training was initiated with the treadmill set at 70% of VO2 max (see tables) and 5 degrees grade for 20 minutes. The duration of exercise was increased by one minute each day until day 31 of training (start of week 7), when a duration of 50 min was reached. Speed and grade of each training session increased in larger increments due to treadmill parameters. The highest intensity and duration of training began on day 31. This intensity was maintained for the final 10 days of the protocol to ensure steady state had been achieved. If any rats were unable to perform at least 4 days of training per week they were removed from the study and euthanized. It is important to note that the starting treadmill speed varied depending on the sex and age of the rat. The initial and maximum speeds were based on VO2max measurements obtained during the pre-training testing of the compliant rats. Rats assigned to the control group followed a schedule similar to the training group. They were placed in one lane on the treadmill for 15 minutes/day, 5 days per week. The treadmill was set at 0 m/min at an incline that corresponded to the incline being used by the training group.
Institute	Broad Institute of MIT and Harvard
Department	Metabolomics Platform
Laboratory	Clish
Last Name	Clish
First Name	Clary
Address	415 Main St, Cambridge, MA 02142
Email	clary@broadinstitute.org
Phone	(617) 714-7654
Submit Date	2023-05-01
Raw Data Available	Yes
Raw Data File Type(s)	TBD
Analysis Type Detail	LC-MS
Release Date	2023-10-06
Release Version	1

Select appropriate tab below to view additional metadata details:

Factors:

Subject type: Mammal; Subject species: Rattus norvegicus (Factor headings shown in green)

mb_sample_id	local_sample_id	Group	Timepoint	Sex
SA264036	90245016003	Control	8 weeks of training or control time	Female
SA264037	90248016003	Control	8 weeks of training or control time	Female
SA264038	90252016003	Control	8 weeks of training or control time	Female
SA264039	90266016003	Control	8 weeks of training or control time	Female
SA264040	90265016003	Control	8 weeks of training or control time	Female
SA264041	90237016003	Control	8 weeks of training or control time	Male
SA264042	90229016003	Control	8 weeks of training or control time	Male
SA264043	90239016003	Control	8 weeks of training or control time	Male
SA264044	90217016003	Control	8 weeks of training or control time	Male
SA264045	90232016003	Control	8 weeks of training or control time	Male
SA264046	QC_PREFA03	QC-DriftCorrection	0 hour	-
SA264047	QC_PREFA01	QC-DriftCorrection	0 hour	-
SA264048	QC_PREFA02	QC-DriftCorrection	0 hour	-
SA264049	QC_PREFA04	QC-DriftCorrection	0 hour	-
SA264050	QC_PREFB02	QC-Pooled	0 hour	-
SA264051	QC_PREFB04	QC-Pooled	0 hour	-
SA264052	QC_PREFB01	QC-Pooled	0 hour	-
SA264053	QC_PREFB03	QC-Pooled	0 hour	-
SA264054	QC_Sed02	QC-Reference	0 hour	-
SA264055	QC_IPE01	QC-Reference	0 hour	-
SA264056	QC_IPE04	QC-Reference	0 hour	-
SA264057	QC_IPE03	QC-Reference	0 hour	-
SA264058	QC_Sed01	QC-Reference	0 hour	-
SA264059	QC_Sed04	QC-Reference	0 hour	-
SA264060	QC_IPE02	QC-Reference	0 hour	-
SA264061	QC_Sed03	QC-Reference	0 hour	-
SA264062	90564016003	Training	1 week of training or control time	Female
SA264063	90567016003	Training	1 week of training or control time	Female
SA264064	90559016003	Training	1 week of training or control time	Female
SA264065	90560016003	Training	1 week of training or control time	Female
SA264066	90571016003	Training	1 week of training or control time	Female
SA264067	90423016003	Training	1 week of training or control time	Male
SA264068	90426016003	Training	1 week of training or control time	Male
SA264069	90430016003	Training	1 week of training or control time	Male
SA264070	90421016003	Training	1 week of training or control time	Male
SA264071	90422016003	Training	1 week of training or control time	Male
SA264072	90578016003	Training	2 weeks of training	Female
SA264073	90587016003	Training	2 weeks of training	Female
SA264074	90581016003	Training	2 weeks of training	Female
SA264075	90585016003	Training	2 weeks of training	Female
SA264076	90576016003	Training	2 weeks of training	Female
SA264077	90449016003	Training	2 weeks of training	Male
SA264078	90444016003	Training	2 weeks of training	Male
SA264079	90439016003	Training	2 weeks of training	Male
SA264080	90450016003	Training	2 weeks of training	Male
SA264081	90441016003	Training	2 weeks of training	Male
SA264082	90406016003	Training	4 weeks of training	Female
SA264083	90420016003	Training	4 weeks of training	Female
SA264084	90410016003	Training	4 weeks of training	Female
SA264085	90412016003	Training	4 weeks of training	Female
SA264086	90416016003	Training	4 weeks of training	Female
SA264087	90280016003	Training	4 weeks of training	Male
SA264088	90281016003	Training	4 weeks of training	Male
SA264089	90289016003	Training	4 weeks of training	Male
SA264090	90292016003	Training	4 weeks of training	Male
SA264091	90283016003	Training	4 weeks of training	Male
SA264092	90251016003	Training	8 weeks of training or control time	Female
SA264093	90254016003	Training	8 weeks of training or control time	Female
SA264094	90267016003	Training	8 weeks of training or control time	Female
SA264095	90258016003	Training	8 weeks of training or control time	Female
SA264096	90259016003	Training	8 weeks of training or control time	Female
SA264097	90222016003	Training	8 weeks of training or control time	Male
SA264098	90218016003	Training	8 weeks of training or control time	Male
SA264099	90225016003	Training	8 weeks of training or control time	Male
SA264100	90227016003	Training	8 weeks of training or control time	Male
SA264101	90223016003	Training	8 weeks of training or control time	Male

Showing results 1 to 66 of 66

Showing results 1 to 66 of 66