Summary of Study ST002665

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001020. The data can be accessed directly via it's Project DOI: 10.21228/M8V97D This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

Study ID	ST002665
Study Title	MoTrPAC: Endurance exercise training study in young adult rats, Tissue:Spleen - Untargeted HILIC-Positive
Study Summary	The goal of the endurance exercise training study in young adult rats (internal code: PASS1B-06) was to perform exercise training studies in adult (6 month) F344 rats, and from these rats collect as many tissues as feasible in order to provide high quality samples for detailed analysis by chemical analysis sites. Tissues were collected from 10-12 rats sedentary control rats concurrent with the collection of the 8-week training groups. The 8-week training group and controls were from the same cohort and same age at euthanasia (either 8). For the older age group, an additional set of controls (n=5-6) were collected with the 1-2 week training group. Rats were either sedentary or underwent an exercise training program. Rats were exercised on the rodent treadmill 5 days per week using a progressive training protocol designed to exercise the rats at approximately 70% of VO2max as outlined in the Table on the next page. Training was performed no earlier than 10:00 am and no later than 5:00 pm over 5 consecutive days per week. Training was initiated with the treadmill set at 70% of VO2 max (see tables) and 5 degrees grade for 20 minutes. The duration of exercise was increased by one minute each day until day 31 of training (start of week 7), when a duration of 50 min was reached. Speed and grade of each training session increased in larger increments due to treadmill parameters. The highest intensity and duration of training began on day 31. This intensity was maintained for the final 10 days of the protocol to ensure steady state had been achieved. If any rats were unable to perform at least 4 days of training per week they were removed from the study and euthanized. It is important to note that the starting treadmill speed varied depending on the sex and age of the rat. The initial and maximum speeds were based on VO2max measurements obtained during the pre-training testing of the compliant rats. Rats assigned to the control group followed a schedule similar to the training group. They were placed in one lane on the treadmill for 15 minutes/day, 5 days per week. The treadmill was set at 0 m/min at an incline that corresponded to the incline being used by the training group.
Institute	Broad Institute of MIT and Harvard
Department	Metabolomics Platform
Laboratory	Clish
Last Name	Clish
First Name	Clary
Address	415 Main St, Cambridge, MA 02142
Email	clary@broadinstitute.org
Phone	(617) 714-7654
Submit Date	2023-05-02
Raw Data Available	Yes
Raw Data File Type(s)	TBD
Analysis Type Detail	LC-MS
Release Date	2023-10-06
Release Version	1

Select appropriate tab below to view additional metadata details:

Factors:

Subject type: Mammal; Subject species: Rattus norvegicus (Factor headings shown in green)

mb_sample_id	local_sample_id	Group	Timepoint	Sex
SA264397	90245016203	Control	8 weeks of training or control time	Female
SA264398	90248016203	Control	8 weeks of training or control time	Female
SA264399	90252016203	Control	8 weeks of training or control time	Female
SA264400	90266016203	Control	8 weeks of training or control time	Female
SA264401	90265016203	Control	8 weeks of training or control time	Female
SA264402	90237016203	Control	8 weeks of training or control time	Male
SA264403	90229016203	Control	8 weeks of training or control time	Male
SA264404	90239016203	Control	8 weeks of training or control time	Male
SA264405	90217016203	Control	8 weeks of training or control time	Male
SA264406	90232016203	Control	8 weeks of training or control time	Male
SA264407	QC_PREFA03	QC-DriftCorrection	0 hour	-
SA264408	QC_PREFA01	QC-DriftCorrection	0 hour	-
SA264409	QC_PREFA02	QC-DriftCorrection	0 hour	-
SA264410	QC_PREFA04	QC-DriftCorrection	0 hour	-
SA264411	QC_PREFB02	QC-Pooled	0 hour	-
SA264412	QC_PREFB04	QC-Pooled	0 hour	-
SA264413	QC_PREFB01	QC-Pooled	0 hour	-
SA264414	QC_PREFB03	QC-Pooled	0 hour	-
SA264415	QC_Sed02	QC-Reference	0 hour	-
SA264416	QC_IPE01	QC-Reference	0 hour	-
SA264417	QC_IPE04	QC-Reference	0 hour	-
SA264418	QC_IPE03	QC-Reference	0 hour	-
SA264419	QC_Sed01	QC-Reference	0 hour	-
SA264420	QC_Sed04	QC-Reference	0 hour	-
SA264421	QC_IPE02	QC-Reference	0 hour	-
SA264422	QC_Sed03	QC-Reference	0 hour	-
SA264423	90564016203	Training	1 week of training or control time	Female
SA264424	90567016203	Training	1 week of training or control time	Female
SA264425	90559016203	Training	1 week of training or control time	Female
SA264426	90560016203	Training	1 week of training or control time	Female
SA264427	90571016203	Training	1 week of training or control time	Female
SA264428	90423016203	Training	1 week of training or control time	Male
SA264429	90426016203	Training	1 week of training or control time	Male
SA264430	90430016203	Training	1 week of training or control time	Male
SA264431	90421016203	Training	1 week of training or control time	Male
SA264432	90422016203	Training	1 week of training or control time	Male
SA264433	90578016203	Training	2 weeks of training	Female
SA264434	90587016203	Training	2 weeks of training	Female
SA264435	90581016203	Training	2 weeks of training	Female
SA264436	90585016203	Training	2 weeks of training	Female
SA264437	90576016203	Training	2 weeks of training	Female
SA264438	90449016203	Training	2 weeks of training	Male
SA264439	90444016203	Training	2 weeks of training	Male
SA264440	90439016203	Training	2 weeks of training	Male
SA264441	90450016203	Training	2 weeks of training	Male
SA264442	90441016203	Training	2 weeks of training	Male
SA264443	90406016203	Training	4 weeks of training	Female
SA264444	90420016203	Training	4 weeks of training	Female
SA264445	90410016203	Training	4 weeks of training	Female
SA264446	90412016203	Training	4 weeks of training	Female
SA264447	90416016203	Training	4 weeks of training	Female
SA264448	90280016203	Training	4 weeks of training	Male
SA264449	90281016203	Training	4 weeks of training	Male
SA264450	90289016203	Training	4 weeks of training	Male
SA264451	90292016203	Training	4 weeks of training	Male
SA264452	90283016203	Training	4 weeks of training	Male
SA264453	90251016203	Training	8 weeks of training or control time	Female
SA264454	90254016203	Training	8 weeks of training or control time	Female
SA264455	90267016203	Training	8 weeks of training or control time	Female
SA264456	90258016203	Training	8 weeks of training or control time	Female
SA264457	90259016203	Training	8 weeks of training or control time	Female
SA264458	90222016203	Training	8 weeks of training or control time	Male
SA264459	90218016203	Training	8 weeks of training or control time	Male
SA264460	90225016203	Training	8 weeks of training or control time	Male
SA264461	90227016203	Training	8 weeks of training or control time	Male
SA264462	90223016203	Training	8 weeks of training or control time	Male

Showing results 1 to 66 of 66

Showing results 1 to 66 of 66