Summary of Study ST002666

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001020. The data can be accessed directly via it's Project DOI: 10.21228/M8V97D This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

Study ID	ST002666
Study Title	MoTrPAC: Endurance exercise training study in young adult rats, Tissue:Testes - Untargeted HILIC-Positive
Study Summary	The goal of the endurance exercise training study in young adult rats (internal code: PASS1B-06) was to perform exercise training studies in adult (6 month) F344 rats, and from these rats collect as many tissues as feasible in order to provide high quality samples for detailed analysis by chemical analysis sites. Tissues were collected from 10-12 rats sedentary control rats concurrent with the collection of the 8-week training groups. The 8-week training group and controls were from the same cohort and same age at euthanasia (either 8). For the older age group, an additional set of controls (n=5-6) were collected with the 1-2 week training group. Rats were either sedentary or underwent an exercise training program. Rats were exercised on the rodent treadmill 5 days per week using a progressive training protocol designed to exercise the rats at approximately 70% of VO2max as outlined in the Table on the next page. Training was performed no earlier than 10:00 am and no later than 5:00 pm over 5 consecutive days per week. Training was initiated with the treadmill set at 70% of VO2 max (see tables) and 5 degrees grade for 20 minutes. The duration of exercise was increased by one minute each day until day 31 of training (start of week 7), when a duration of 50 min was reached. Speed and grade of each training session increased in larger increments due to treadmill parameters. The highest intensity and duration of training began on day 31. This intensity was maintained for the final 10 days of the protocol to ensure steady state had been achieved. If any rats were unable to perform at least 4 days of training per week they were removed from the study and euthanized. It is important to note that the starting treadmill speed varied depending on the sex and age of the rat. The initial and maximum speeds were based on VO2max measurements obtained during the pre-training testing of the compliant rats. Rats assigned to the control group followed a schedule similar to the training group. They were placed in one lane on the treadmill for 15 minutes/day, 5 days per week. The treadmill was set at 0 m/min at an incline that corresponded to the incline being used by the training group.
Institute	Broad Institute of MIT and Harvard
Department	Metabolomics Platform
Laboratory	Clish
Last Name	Clish
First Name	Clary
Address	415 Main St, Cambridge, MA 02142
Email	clary@broadinstitute.org
Phone	(617) 714-7654
Submit Date	2023-05-02
Raw Data Available	Yes
Raw Data File Type(s)	TBD
Analysis Type Detail	LC-MS
Release Date	2023-10-06
Release Version	1

Select appropriate tab below to view additional metadata details:

Factors:

Subject type: Mammal; Subject species: Rattus norvegicus (Factor headings shown in green)

mb_sample_id	local_sample_id	Group	Timepoint	Sex
SA264463	90229016303	Control	8 weeks of training or control time	Male
SA264464	90232016303	Control	8 weeks of training or control time	Male
SA264465	90239016303	Control	8 weeks of training or control time	Male
SA264466	90217016303	Control	8 weeks of training or control time	Male
SA264467	90237016303	Control	8 weeks of training or control time	Male
SA264468	QC_PREFA01	QC-DriftCorrection	0 hour	-
SA264469	QC_PREFA02	QC-DriftCorrection	0 hour	-
SA264470	QC_PREFA03	QC-DriftCorrection	0 hour	-
SA264471	QC_PREFB01	QC-Pooled	0 hour	-
SA264472	QC_PREFB02	QC-Pooled	0 hour	-
SA264473	QC_PREFB03	QC-Pooled	0 hour	-
SA264474	QC_Sed04	QC-Reference	0 hour	-
SA264475	QC_IPE02	QC-Reference	0 hour	-
SA264476	QC_Sed03	QC-Reference	0 hour	-
SA264477	QC_IPE04	QC-Reference	0 hour	-
SA264478	QC_IPE03	QC-Reference	0 hour	-
SA264479	QC_IPE01	QC-Reference	0 hour	-
SA264480	QC_Sed02	QC-Reference	0 hour	-
SA264481	QC_Sed01	QC-Reference	0 hour	-
SA264482	90426016303	Training	1 week of training or control time	Male
SA264483	90430016303	Training	1 week of training or control time	Male
SA264484	90423016303	Training	1 week of training or control time	Male
SA264485	90422016303	Training	1 week of training or control time	Male
SA264486	90421016303	Training	1 week of training or control time	Male
SA264487	90441016303	Training	2 weeks of training	Male
SA264488	90439016303	Training	2 weeks of training	Male
SA264489	90449016303	Training	2 weeks of training	Male
SA264490	90444016303	Training	2 weeks of training	Male
SA264491	90450016303	Training	2 weeks of training	Male
SA264492	90289016303	Training	4 weeks of training	Male
SA264493	90292016303	Training	4 weeks of training	Male
SA264494	90280016303	Training	4 weeks of training	Male
SA264495	90281016303	Training	4 weeks of training	Male
SA264496	90283016303	Training	4 weeks of training	Male
SA264497	90218016303	Training	8 weeks of training or control time	Male
SA264498	90222016303	Training	8 weeks of training or control time	Male
SA264499	90227016303	Training	8 weeks of training or control time	Male
SA264500	90225016303	Training	8 weeks of training or control time	Male
SA264501	90223016303	Training	8 weeks of training or control time	Male

Showing results 1 to 39 of 39

Showing results 1 to 39 of 39