Summary of Study ST002667

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001020. The data can be accessed directly via it's Project DOI: 10.21228/M8V97D This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

Study ID	ST002667
Study Title	MoTrPAC: Endurance exercise training study in young adult rats, Tissue:Ovaries - Untargeted HILIC-Positive
Study Summary	The goal of the endurance exercise training study in young adult rats (internal code: PASS1B-06) was to perform exercise training studies in adult (6 month) F344 rats, and from these rats collect as many tissues as feasible in order to provide high quality samples for detailed analysis by chemical analysis sites. Tissues were collected from 10-12 rats sedentary control rats concurrent with the collection of the 8-week training groups. The 8-week training group and controls were from the same cohort and same age at euthanasia (either 8). For the older age group, an additional set of controls (n=5-6) were collected with the 1-2 week training group. Rats were either sedentary or underwent an exercise training program. Rats were exercised on the rodent treadmill 5 days per week using a progressive training protocol designed to exercise the rats at approximately 70% of VO2max as outlined in the Table on the next page. Training was performed no earlier than 10:00 am and no later than 5:00 pm over 5 consecutive days per week. Training was initiated with the treadmill set at 70% of VO2 max (see tables) and 5 degrees grade for 20 minutes. The duration of exercise was increased by one minute each day until day 31 of training (start of week 7), when a duration of 50 min was reached. Speed and grade of each training session increased in larger increments due to treadmill parameters. The highest intensity and duration of training began on day 31. This intensity was maintained for the final 10 days of the protocol to ensure steady state had been achieved. If any rats were unable to perform at least 4 days of training per week they were removed from the study and euthanized. It is important to note that the starting treadmill speed varied depending on the sex and age of the rat. The initial and maximum speeds were based on VO2max measurements obtained during the pre-training testing of the compliant rats. Rats assigned to the control group followed a schedule similar to the training group. They were placed in one lane on the treadmill for 15 minutes/day, 5 days per week. The treadmill was set at 0 m/min at an incline that corresponded to the incline being used by the training group.
Institute	Broad Institute of MIT and Harvard
Department	Metabolomics Platform
Laboratory	Clish
Last Name	Clish
First Name	Clary
Address	415 Main St, Cambridge, MA 02142
Email	clary@broadinstitute.org
Phone	(617) 714-7654
Submit Date	2023-05-02
Raw Data Available	Yes
Raw Data File Type(s)	TBD
Analysis Type Detail	LC-MS
Release Date	2023-10-06
Release Version	1

Select appropriate tab below to view additional metadata details:

Factors:

Subject type: Mammal; Subject species: Rattus norvegicus (Factor headings shown in green)

mb_sample_id	local_sample_id	Group	Timepoint	Sex
SA264502	90252016403	Control	8 weeks of training or control time	Female
SA264503	90265016403	Control	8 weeks of training or control time	Female
SA264504	90266016403	Control	8 weeks of training or control time	Female
SA264505	90248016403	Control	8 weeks of training or control time	Female
SA264506	90245016403	Control	8 weeks of training or control time	Female
SA264507	QC_PREFA01	QC-DriftCorrection	0 hour	-
SA264508	QC_PREFA02	QC-DriftCorrection	0 hour	-
SA264509	QC_PREFA03	QC-DriftCorrection	0 hour	-
SA264510	QC_PREFB01	QC-Pooled	0 hour	-
SA264511	QC_PREFB03	QC-Pooled	0 hour	-
SA264512	QC_PREFB02	QC-Pooled	0 hour	-
SA264513	90560016403	Training	1 week of training or control time	Female
SA264514	90571016403	Training	1 week of training or control time	Female
SA264515	90559016403	Training	1 week of training or control time	Female
SA264516	90567016403	Training	1 week of training or control time	Female
SA264517	90564016403	Training	1 week of training or control time	Female
SA264518	90581016403	Training	2 weeks of training	Female
SA264519	90587016403	Training	2 weeks of training	Female
SA264520	90585016403	Training	2 weeks of training	Female
SA264521	90578016403	Training	2 weeks of training	Female
SA264522	90576016403	Training	2 weeks of training	Female
SA264523	90416016403	Training	4 weeks of training	Female
SA264524	90420016403	Training	4 weeks of training	Female
SA264525	90406016403	Training	4 weeks of training	Female
SA264526	90410016403	Training	4 weeks of training	Female
SA264527	90412016403	Training	4 weeks of training	Female
SA264528	90251016403	Training	8 weeks of training or control time	Female
SA264529	90254016403	Training	8 weeks of training or control time	Female
SA264530	90267016403	Training	8 weeks of training or control time	Female
SA264531	90259016403	Training	8 weeks of training or control time	Female
SA264532	90258016403	Training	8 weeks of training or control time	Female

Showing results 1 to 31 of 31

Showing results 1 to 31 of 31