Summary of Study ST002675

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001020. The data can be accessed directly via it's Project DOI: 10.21228/M8V97D This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

Study ID	ST002675
Study Title	MoTrPAC: Endurance exercise training study in young adult rats, Tissue:Liver - Untargeted HILIC-Positive
Study Summary	The goal of the endurance exercise training study in young adult rats (internal code: PASS1B-06) was to perform exercise training studies in adult (6 month) F344 rats, and from these rats collect as many tissues as feasible in order to provide high quality samples for detailed analysis by chemical analysis sites. Tissues were collected from 10-12 rats sedentary control rats concurrent with the collection of the 8-week training groups. The 8-week training group and controls were from the same cohort and same age at euthanasia (either 8). For the older age group, an additional set of controls (n=5-6) were collected with the 1-2 week training group. Rats were either sedentary or underwent an exercise training program. Rats were exercised on the rodent treadmill 5 days per week using a progressive training protocol designed to exercise the rats at approximately 70% of VO2max as outlined in the Table on the next page. Training was performed no earlier than 10:00 am and no later than 5:00 pm over 5 consecutive days per week. Training was initiated with the treadmill set at 70% of VO2 max (see tables) and 5 degrees grade for 20 minutes. The duration of exercise was increased by one minute each day until day 31 of training (start of week 7), when a duration of 50 min was reached. Speed and grade of each training session increased in larger increments due to treadmill parameters. The highest intensity and duration of training began on day 31. This intensity was maintained for the final 10 days of the protocol to ensure steady state had been achieved. If any rats were unable to perform at least 4 days of training per week they were removed from the study and euthanized. It is important to note that the starting treadmill speed varied depending on the sex and age of the rat. The initial and maximum speeds were based on VO2max measurements obtained during the pre-training testing of the compliant rats. Rats assigned to the control group followed a schedule similar to the training group. They were placed in one lane on the treadmill for 15 minutes/day, 5 days per week. The treadmill was set at 0 m/min at an incline that corresponded to the incline being used by the training group.
Institute	Broad Institute of MIT and Harvard
Department	Metabolomics Platform
Laboratory	Clish
Last Name	Clish
First Name	Clary
Address	415 Main St, Cambridge, MA 02142
Email	clary@broadinstitute.org
Phone	(617) 714-7654
Submit Date	2023-05-02
Raw Data Available	Yes
Raw Data File Type(s)	TBD
Analysis Type Detail	LC-MS
Release Date	2023-10-06
Release Version	1

Select appropriate tab below to view additional metadata details:

Factors:

Subject type: Mammal; Subject species: Rattus norvegicus (Factor headings shown in green)

mb_sample_id	local_sample_id	Group	Timepoint	Sex
SA265075	90245016807	Control	8 weeks of training or control time	Female
SA265076	90248016807	Control	8 weeks of training or control time	Female
SA265077	90252016807	Control	8 weeks of training or control time	Female
SA265078	90266016807	Control	8 weeks of training or control time	Female
SA265079	90265016807	Control	8 weeks of training or control time	Female
SA265080	90237016807	Control	8 weeks of training or control time	Male
SA265081	90229016807	Control	8 weeks of training or control time	Male
SA265082	90239016807	Control	8 weeks of training or control time	Male
SA265083	90217016807	Control	8 weeks of training or control time	Male
SA265084	90232016807	Control	8 weeks of training or control time	Male
SA265085	QC_PREFA03	QC-DriftCorrection	0 hour	-
SA265086	QC_PREFA01	QC-DriftCorrection	0 hour	-
SA265087	QC_PREFA02	QC-DriftCorrection	0 hour	-
SA265088	QC_PREFA04	QC-DriftCorrection	0 hour	-
SA265089	QC_PREFB02	QC-Pooled	0 hour	-
SA265090	QC_PREFB04	QC-Pooled	0 hour	-
SA265091	QC_PREFB01	QC-Pooled	0 hour	-
SA265092	QC_PREFB03	QC-Pooled	0 hour	-
SA265093	QC_Sed02	QC-Reference	0 hour	-
SA265094	QC_IPE01	QC-Reference	0 hour	-
SA265095	QC_IPE04	QC-Reference	0 hour	-
SA265096	QC_IPE03	QC-Reference	0 hour	-
SA265097	QC_Sed01	QC-Reference	0 hour	-
SA265098	QC_Sed04	QC-Reference	0 hour	-
SA265099	QC_IPE02	QC-Reference	0 hour	-
SA265100	QC_Sed03	QC-Reference	0 hour	-
SA265101	90564016807	Training	1 week of training or control time	Female
SA265102	90567016807	Training	1 week of training or control time	Female
SA265103	90559016807	Training	1 week of training or control time	Female
SA265104	90560016807	Training	1 week of training or control time	Female
SA265105	90571016807	Training	1 week of training or control time	Female
SA265106	90423016807	Training	1 week of training or control time	Male
SA265107	90426016807	Training	1 week of training or control time	Male
SA265108	90430016807	Training	1 week of training or control time	Male
SA265109	90421016807	Training	1 week of training or control time	Male
SA265110	90422016807	Training	1 week of training or control time	Male
SA265111	90578016807	Training	2 weeks of training	Female
SA265112	90587016807	Training	2 weeks of training	Female
SA265113	90581016807	Training	2 weeks of training	Female
SA265114	90585016807	Training	2 weeks of training	Female
SA265115	90576016807	Training	2 weeks of training	Female
SA265116	90449016807	Training	2 weeks of training	Male
SA265117	90444016807	Training	2 weeks of training	Male
SA265118	90439016807	Training	2 weeks of training	Male
SA265119	90450016807	Training	2 weeks of training	Male
SA265120	90441016807	Training	2 weeks of training	Male
SA265121	90406016807	Training	4 weeks of training	Female
SA265122	90420016807	Training	4 weeks of training	Female
SA265123	90410016807	Training	4 weeks of training	Female
SA265124	90412016807	Training	4 weeks of training	Female
SA265125	90416016807	Training	4 weeks of training	Female
SA265126	90280016807	Training	4 weeks of training	Male
SA265127	90281016807	Training	4 weeks of training	Male
SA265128	90289016807	Training	4 weeks of training	Male
SA265129	90292016807	Training	4 weeks of training	Male
SA265130	90283016807	Training	4 weeks of training	Male
SA265131	90251016807	Training	8 weeks of training or control time	Female
SA265132	90254016807	Training	8 weeks of training or control time	Female
SA265133	90267016807	Training	8 weeks of training or control time	Female
SA265134	90258016807	Training	8 weeks of training or control time	Female
SA265135	90259016807	Training	8 weeks of training or control time	Female
SA265136	90222016807	Training	8 weeks of training or control time	Male
SA265137	90218016807	Training	8 weeks of training or control time	Male
SA265138	90225016807	Training	8 weeks of training or control time	Male
SA265139	90227016807	Training	8 weeks of training or control time	Male
SA265140	90223016807	Training	8 weeks of training or control time	Male

Showing results 1 to 66 of 66

Showing results 1 to 66 of 66