Summary of Study ST002682

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001020. The data can be accessed directly via it's Project DOI: 10.21228/M8V97D This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

Study ID	ST002682
Study Title	MoTrPAC: Endurance exercise training study in young adult rats, Tissue:Brown Adipose - Untargeted HILIC-Positive
Study Summary	The goal of the endurance exercise training study in young adult rats (internal code: PASS1B-06) was to perform exercise training studies in adult (6 month) F344 rats, and from these rats collect as many tissues as feasible in order to provide high quality samples for detailed analysis by chemical analysis sites. Tissues were collected from 10-12 rats sedentary control rats concurrent with the collection of the 8-week training groups. The 8-week training group and controls were from the same cohort and same age at euthanasia (either 8). For the older age group, an additional set of controls (n=5-6) were collected with the 1-2 week training group. Rats were either sedentary or underwent an exercise training program. Rats were exercised on the rodent treadmill 5 days per week using a progressive training protocol designed to exercise the rats at approximately 70% of VO2max as outlined in the Table on the next page. Training was performed no earlier than 10:00 am and no later than 5:00 pm over 5 consecutive days per week. Training was initiated with the treadmill set at 70% of VO2 max (see tables) and 5 degrees grade for 20 minutes. The duration of exercise was increased by one minute each day until day 31 of training (start of week 7), when a duration of 50 min was reached. Speed and grade of each training session increased in larger increments due to treadmill parameters. The highest intensity and duration of training began on day 31. This intensity was maintained for the final 10 days of the protocol to ensure steady state had been achieved. If any rats were unable to perform at least 4 days of training per week they were removed from the study and euthanized. It is important to note that the starting treadmill speed varied depending on the sex and age of the rat. The initial and maximum speeds were based on VO2max measurements obtained during the pre-training testing of the compliant rats. Rats assigned to the control group followed a schedule similar to the training group. They were placed in one lane on the treadmill for 15 minutes/day, 5 days per week. The treadmill was set at 0 m/min at an incline that corresponded to the incline being used by the training group.
Institute	Broad Institute of MIT and Harvard
Department	Metabolomics Platform
Laboratory	Clish
Last Name	Clish
First Name	Clary
Address	415 Main St, Cambridge, MA 02142
Email	clary@broadinstitute.org
Phone	(617) 714-7654
Submit Date	2023-05-02
Raw Data Available	Yes
Raw Data File Type(s)	TBD
Analysis Type Detail	LC-MS
Release Date	2023-10-06
Release Version	1

Select appropriate tab below to view additional metadata details:

Factors:

Subject type: Mammal; Subject species: Rattus norvegicus (Factor headings shown in green)

mb_sample_id	local_sample_id	Group	Timepoint	Sex
SA265682	90245016905	Control	8 weeks of training or control time	Female
SA265683	90248016905	Control	8 weeks of training or control time	Female
SA265684	90252016905	Control	8 weeks of training or control time	Female
SA265685	90265016905	Control	8 weeks of training or control time	Female
SA265686	90266016905	Control	8 weeks of training or control time	Female
SA265687	90237016905	Control	8 weeks of training or control time	Male
SA265688	90229016905	Control	8 weeks of training or control time	Male
SA265689	90239016905	Control	8 weeks of training or control time	Male
SA265690	90217016905	Control	8 weeks of training or control time	Male
SA265691	90232016905	Control	8 weeks of training or control time	Male
SA265692	QC_PREFA03	QC-DriftCorrection	0 hour	-
SA265693	QC_PREFA01	QC-DriftCorrection	0 hour	-
SA265694	QC_PREFA02	QC-DriftCorrection	0 hour	-
SA265695	QC_PREFA04	QC-DriftCorrection	0 hour	-
SA265696	QC_PREFB03	QC-Pooled	0 hour	-
SA265697	QC_PREFB01	QC-Pooled	0 hour	-
SA265698	QC_PREFB02	QC-Pooled	0 hour	-
SA265699	QC_PREFB04	QC-Pooled	0 hour	-
SA265700	QC_IPE03	QC-Reference	0 hour	-
SA265701	QC_IPE02	QC-Reference	0 hour	-
SA265702	QC_Sed03	QC-Reference	0 hour	-
SA265703	QC_Sed02	QC-Reference	0 hour	-
SA265704	QC_IPE04	QC-Reference	0 hour	-
SA265705	QC_Sed04	QC-Reference	0 hour	-
SA265706	QC_Sed01	QC-Reference	0 hour	-
SA265707	QC_IPE01	QC-Reference	0 hour	-
SA265708	90567016905	Training	1 week of training or control time	Female
SA265709	90564016905	Training	1 week of training or control time	Female
SA265710	90560016905	Training	1 week of training or control time	Female
SA265711	90571016905	Training	1 week of training or control time	Female
SA265712	90559016905	Training	1 week of training or control time	Female
SA265713	90423016905	Training	1 week of training or control time	Male
SA265714	90426016905	Training	1 week of training or control time	Male
SA265715	90430016905	Training	1 week of training or control time	Male
SA265716	90421016905	Training	1 week of training or control time	Male
SA265717	90422016905	Training	1 week of training or control time	Male
SA265718	90587016905	Training	2 weeks of training	Female
SA265719	90581016905	Training	2 weeks of training	Female
SA265720	90578016905	Training	2 weeks of training	Female
SA265721	90585016905	Training	2 weeks of training	Female
SA265722	90576016905	Training	2 weeks of training	Female
SA265723	90449016905	Training	2 weeks of training	Male
SA265724	90450016905	Training	2 weeks of training	Male
SA265725	90441016905	Training	2 weeks of training	Male
SA265726	90439016905	Training	2 weeks of training	Male
SA265727	90444016905	Training	2 weeks of training	Male
SA265728	90406016905	Training	4 weeks of training	Female
SA265729	90420016905	Training	4 weeks of training	Female
SA265730	90410016905	Training	4 weeks of training	Female
SA265731	90412016905	Training	4 weeks of training	Female
SA265732	90416016905	Training	4 weeks of training	Female
SA265733	90281016905	Training	4 weeks of training	Male
SA265734	90283016905	Training	4 weeks of training	Male
SA265735	90280016905	Training	4 weeks of training	Male
SA265736	90289016905	Training	4 weeks of training	Male
SA265737	90292016905	Training	4 weeks of training	Male
SA265738	90254016905	Training	8 weeks of training or control time	Female
SA265739	90251016905	Training	8 weeks of training or control time	Female
SA265740	90258016905	Training	8 weeks of training or control time	Female
SA265741	90267016905	Training	8 weeks of training or control time	Female
SA265742	90222016905	Training	8 weeks of training or control time	Male
SA265743	90218016905	Training	8 weeks of training or control time	Male
SA265744	90225016905	Training	8 weeks of training or control time	Male
SA265745	90227016905	Training	8 weeks of training or control time	Male
SA265746	90223016905	Training	8 weeks of training or control time	Male

Showing results 1 to 65 of 65

Showing results 1 to 65 of 65