Summary of Study ST002685

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001020. The data can be accessed directly via it's Project DOI: 10.21228/M8V97D This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002685
Study TitleMoTrPAC: Endurance exercise training study in young adult rats, Tissue:Brown Adipose - Untargeted Lipidomics, Reversed-Phase Positive
Study SummaryThe goal of the endurance exercise training study in young adult rats (internal code: PASS1B-06) was to perform exercise training studies in adult (6 month) F344 rats, and from these rats collect as many tissues as feasible in order to provide high quality samples for detailed analysis by chemical analysis sites. Tissues were collected from 10-12 rats sedentary control rats concurrent with the collection of the 8-week training groups. The 8-week training group and controls were from the same cohort and same age at euthanasia (either 8). For the older age group, an additional set of controls (n=5-6) were collected with the 1-2 week training group. Rats were either sedentary or underwent an exercise training program. Rats were exercised on the rodent treadmill 5 days per week using a progressive training protocol designed to exercise the rats at approximately 70% of VO2max as outlined in the Table on the next page. Training was performed no earlier than 10:00 am and no later than 5:00 pm over 5 consecutive days per week. Training was initiated with the treadmill set at 70% of VO2 max (see tables) and 5 degrees grade for 20 minutes. The duration of exercise was increased by one minute each day until day 31 of training (start of week 7), when a duration of 50 min was reached. Speed and grade of each training session increased in larger increments due to treadmill parameters. The highest intensity and duration of training began on day 31. This intensity was maintained for the final 10 days of the protocol to ensure steady state had been achieved. If any rats were unable to perform at least 4 days of training per week they were removed from the study and euthanized. It is important to note that the starting treadmill speed varied depending on the sex and age of the rat. The initial and maximum speeds were based on VO2max measurements obtained during the pre-training testing of the compliant rats. Rats assigned to the control group followed a schedule similar to the training group. They were placed in one lane on the treadmill for 15 minutes/day, 5 days per week. The treadmill was set at 0 m/min at an incline that corresponded to the incline being used by the training group.
Institute
Georgia Institute of Technology
DepartmentDepartment of Chemistry and Biochemistry
LaboratorySystems Mass Spectrometry Core (SyMSC)
Last NameFacundo
First NameFernandez
Address901 Atlantic Dr NW, Atlanta, GA 30332-0400
Emailfacundo.fernandez@chemistry.gatech.edu
Phone(404) 385-4432
Submit Date2023-05-02
Raw Data AvailableYes
Raw Data File Type(s)TBD
Analysis Type DetailLC-MS
Release Date2023-10-06
Release Version1
Fernandez Facundo Fernandez Facundo
https://dx.doi.org/10.21228/M8V97D
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:


Factors:

Subject type: Mammal; Subject species: Rattus norvegicus (Factor headings shown in green)

mb_sample_id local_sample_id Group Timepoint Sex
SA26588590252016907Control 8 weeks of training or control time Female
SA26588690266016907Control 8 weeks of training or control time Female
SA26588790265016907Control 8 weeks of training or control time Female
SA26588890239016907Control 8 weeks of training or control time Male
SA26588990237016907Control 8 weeks of training or control time Male
SA26589090217016907Control 8 weeks of training or control time Male
SA26589190229016907Control 8 weeks of training or control time Male
SA26589290232016907Control 8 weeks of training or control time Male
SA265893IPE_Reference_BAT02QC-Blank 0 hour -
SA265894SampleBlank04QC-Blank 0 hour -
SA265895SampleBlank03QC-Blank 0 hour -
SA265896SampleBlank05QC-Blank 0 hour -
SA265897SampleBlank01QC-Blank 0 hour -
SA265898SampleBlank02QC-Blank 0 hour -
SA265899QC01QC-Pooled 0 hour -
SA265900QC05QC-Pooled 0 hour -
SA265901QC09QC-Pooled 0 hour -
SA265902QC07QC-Pooled 0 hour -
SA265903QC06QC-Pooled 0 hour -
SA265904SampleBlank06QC-Pooled 0 hour -
SA265905QC04QC-Pooled 0 hour -
SA265906QC03QC-Pooled 0 hour -
SA265907QC02QC-Pooled 0 hour -
SA265908SED_Reference_BATQC-Reference 0 hour -
SA265909SED_Reference_BAT02QC-Reference 0 hour -
SA265910IPE_Reference_BATQC-Reference 0 hour -
SA26591190567016907Training 1 week of training or control time Female
SA26591290571016907Training 1 week of training or control time Female
SA26591390560016907Training 1 week of training or control time Female
SA26591490559016907Training 1 week of training or control time Female
SA26591590430016907Training 1 week of training or control time Male
SA26591690421016907Training 1 week of training or control time Male
SA26591790422016907Training 1 week of training or control time Male
SA26591890423016907Training 1 week of training or control time Male
SA26591990426016907Training 1 week of training or control time Male
SA26592090576016907Training 2 weeks of training Female
SA26592190585016907Training 2 weeks of training Female
SA26592290581016907Training 2 weeks of training Female
SA26592390578016907Training 2 weeks of training Female
SA26592490587016907Training 2 weeks of training Female
SA26592590444016907Training 2 weeks of training Male
SA26592690450016907Training 2 weeks of training Male
SA26592790449016907Training 2 weeks of training Male
SA26592890439016907Training 2 weeks of training Male
SA26592990441016907Training 2 weeks of training Male
SA26593090416016907Training 4 weeks of training Female
SA26593190406016907Training 4 weeks of training Female
SA26593290410016907Training 4 weeks of training Female
SA26593390420016907Training 4 weeks of training Female
SA26593490412016907Training 4 weeks of training Female
SA26593590283016907Training 4 weeks of training Male
SA26593690281016907Training 4 weeks of training Male
SA26593790289016907Training 4 weeks of training Male
SA26593890280016907Training 4 weeks of training Male
SA26593990251016907Training 8 weeks of training or control time Female
SA26594090254016907Training 8 weeks of training or control time Female
SA26594190225016907Training 8 weeks of training or control time Male
Showing results 1 to 57 of 57
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