Summary of Study ST002688

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001020. The data can be accessed directly via it's Project DOI: 10.21228/M8V97D This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

Study ID	ST002688
Study Title	MoTrPAC: Endurance exercise training study in young adult rats, Tissue:White Adipose - Untargeted HILIC-Positive
Study Summary	The goal of the endurance exercise training study in young adult rats (internal code: PASS1B-06) was to perform exercise training studies in adult (6 month) F344 rats, and from these rats collect as many tissues as feasible in order to provide high quality samples for detailed analysis by chemical analysis sites. Tissues were collected from 10-12 rats sedentary control rats concurrent with the collection of the 8-week training groups. The 8-week training group and controls were from the same cohort and same age at euthanasia (either 8). For the older age group, an additional set of controls (n=5-6) were collected with the 1-2 week training group. Rats were either sedentary or underwent an exercise training program. Rats were exercised on the rodent treadmill 5 days per week using a progressive training protocol designed to exercise the rats at approximately 70% of VO2max as outlined in the Table on the next page. Training was performed no earlier than 10:00 am and no later than 5:00 pm over 5 consecutive days per week. Training was initiated with the treadmill set at 70% of VO2 max (see tables) and 5 degrees grade for 20 minutes. The duration of exercise was increased by one minute each day until day 31 of training (start of week 7), when a duration of 50 min was reached. Speed and grade of each training session increased in larger increments due to treadmill parameters. The highest intensity and duration of training began on day 31. This intensity was maintained for the final 10 days of the protocol to ensure steady state had been achieved. If any rats were unable to perform at least 4 days of training per week they were removed from the study and euthanized. It is important to note that the starting treadmill speed varied depending on the sex and age of the rat. The initial and maximum speeds were based on VO2max measurements obtained during the pre-training testing of the compliant rats. Rats assigned to the control group followed a schedule similar to the training group. They were placed in one lane on the treadmill for 15 minutes/day, 5 days per week. The treadmill was set at 0 m/min at an incline that corresponded to the incline being used by the training group.
Institute	Broad Institute of MIT and Harvard
Department	Metabolomics Platform
Laboratory	Clish
Last Name	Clish
First Name	Clary
Address	415 Main St, Cambridge, MA 02142
Email	clary@broadinstitute.org
Phone	(617) 714-7654
Submit Date	2023-05-02
Raw Data Available	Yes
Raw Data File Type(s)	TBD
Analysis Type Detail	LC-MS
Release Date	2023-10-06
Release Version	1

Select appropriate tab below to view additional metadata details:

Factors:

Subject type: Mammal; Subject species: Rattus norvegicus (Factor headings shown in green)

mb_sample_id	local_sample_id	Group	Timepoint	Sex
SA266114	90245017007	Control	8 weeks of training or control time	Female
SA266115	90248017007	Control	8 weeks of training or control time	Female
SA266116	90252017007	Control	8 weeks of training or control time	Female
SA266117	90266017007	Control	8 weeks of training or control time	Female
SA266118	90265017007	Control	8 weeks of training or control time	Female
SA266119	90237017007	Control	8 weeks of training or control time	Male
SA266120	90229017007	Control	8 weeks of training or control time	Male
SA266121	90239017007	Control	8 weeks of training or control time	Male
SA266122	90217017007	Control	8 weeks of training or control time	Male
SA266123	90232017007	Control	8 weeks of training or control time	Male
SA266124	QC_PREFA03	QC-DriftCorrection	0 hour	-
SA266125	QC_PREFA01	QC-DriftCorrection	0 hour	-
SA266126	QC_PREFA02	QC-DriftCorrection	0 hour	-
SA266127	QC_PREFA04	QC-DriftCorrection	0 hour	-
SA266128	QC_PREFB02	QC-Pooled	0 hour	-
SA266129	QC_PREFB04	QC-Pooled	0 hour	-
SA266130	QC_PREFB01	QC-Pooled	0 hour	-
SA266131	QC_PREFB03	QC-Pooled	0 hour	-
SA266132	QC_Sed02	QC-Reference	0 hour	-
SA266133	QC_IPE01	QC-Reference	0 hour	-
SA266134	QC_IPE04	QC-Reference	0 hour	-
SA266135	QC_IPE03	QC-Reference	0 hour	-
SA266136	QC_Sed01	QC-Reference	0 hour	-
SA266137	QC_Sed04	QC-Reference	0 hour	-
SA266138	QC_IPE02	QC-Reference	0 hour	-
SA266139	QC_Sed03	QC-Reference	0 hour	-
SA266140	90564017007	Training	1 week of training or control time	Female
SA266141	90567017007	Training	1 week of training or control time	Female
SA266142	90559017007	Training	1 week of training or control time	Female
SA266143	90560017007	Training	1 week of training or control time	Female
SA266144	90571017007	Training	1 week of training or control time	Female
SA266145	90423017007	Training	1 week of training or control time	Male
SA266146	90426017007	Training	1 week of training or control time	Male
SA266147	90430017007	Training	1 week of training or control time	Male
SA266148	90421017007	Training	1 week of training or control time	Male
SA266149	90422017007	Training	1 week of training or control time	Male
SA266150	90578017007	Training	2 weeks of training	Female
SA266151	90587017007	Training	2 weeks of training	Female
SA266152	90581017007	Training	2 weeks of training	Female
SA266153	90585017007	Training	2 weeks of training	Female
SA266154	90576017007	Training	2 weeks of training	Female
SA266155	90449017007	Training	2 weeks of training	Male
SA266156	90444017007	Training	2 weeks of training	Male
SA266157	90439017007	Training	2 weeks of training	Male
SA266158	90450017007	Training	2 weeks of training	Male
SA266159	90441017007	Training	2 weeks of training	Male
SA266160	90406017007	Training	4 weeks of training	Female
SA266161	90420017007	Training	4 weeks of training	Female
SA266162	90410017007	Training	4 weeks of training	Female
SA266163	90412017007	Training	4 weeks of training	Female
SA266164	90416017007	Training	4 weeks of training	Female
SA266165	90280017007	Training	4 weeks of training	Male
SA266166	90281017007	Training	4 weeks of training	Male
SA266167	90289017007	Training	4 weeks of training	Male
SA266168	90292017007	Training	4 weeks of training	Male
SA266169	90283017007	Training	4 weeks of training	Male
SA266170	90251017007	Training	8 weeks of training or control time	Female
SA266171	90254017007	Training	8 weeks of training or control time	Female
SA266172	90267017007	Training	8 weeks of training or control time	Female
SA266173	90258017007	Training	8 weeks of training or control time	Female
SA266174	90259017007	Training	8 weeks of training or control time	Female
SA266175	90222017007	Training	8 weeks of training or control time	Male
SA266176	90218017007	Training	8 weeks of training or control time	Male
SA266177	90225017007	Training	8 weeks of training or control time	Male
SA266178	90227017007	Training	8 weeks of training or control time	Male
SA266179	90223017007	Training	8 weeks of training or control time	Male

Showing results 1 to 66 of 66

Showing results 1 to 66 of 66