Summary of Study ST002694

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001020. The data can be accessed directly via it's Project DOI: 10.21228/M8V97D This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

Study ID	ST002694
Study Title	MoTrPAC: Endurance exercise training study in young adult rats, Tissue:White Adipose - Untargeted Reversed-Phase Positive
Study Summary	The goal of the endurance exercise training study in young adult rats (internal code: PASS1B-06) was to perform exercise training studies in adult (6 month) F344 rats, and from these rats collect as many tissues as feasible in order to provide high quality samples for detailed analysis by chemical analysis sites. Tissues were collected from 10-12 rats sedentary control rats concurrent with the collection of the 8-week training groups. The 8-week training group and controls were from the same cohort and same age at euthanasia (either 8). For the older age group, an additional set of controls (n=5-6) were collected with the 1-2 week training group. Rats were either sedentary or underwent an exercise training program. Rats were exercised on the rodent treadmill 5 days per week using a progressive training protocol designed to exercise the rats at approximately 70% of VO2max as outlined in the Table on the next page. Training was performed no earlier than 10:00 am and no later than 5:00 pm over 5 consecutive days per week. Training was initiated with the treadmill set at 70% of VO2 max (see tables) and 5 degrees grade for 20 minutes. The duration of exercise was increased by one minute each day until day 31 of training (start of week 7), when a duration of 50 min was reached. Speed and grade of each training session increased in larger increments due to treadmill parameters. The highest intensity and duration of training began on day 31. This intensity was maintained for the final 10 days of the protocol to ensure steady state had been achieved. If any rats were unable to perform at least 4 days of training per week they were removed from the study and euthanized. It is important to note that the starting treadmill speed varied depending on the sex and age of the rat. The initial and maximum speeds were based on VO2max measurements obtained during the pre-training testing of the compliant rats. Rats assigned to the control group followed a schedule similar to the training group. They were placed in one lane on the treadmill for 15 minutes/day, 5 days per week. The treadmill was set at 0 m/min at an incline that corresponded to the incline being used by the training group.
Institute	University of Michigan
Department	Biomedical Research Core Facilities
Laboratory	Metabolomics core
Last Name	Burant
First Name	Charles
Address	6120 Brehm Tower,1000 Wall St., Ann Arbor, MI, 48105-5714
Email	burantc@med.umich.edu
Phone	(734) 232-0842
Submit Date	2023-05-02
Num Groups	65
Total Subjects	50
Raw Data Available	Yes
Raw Data File Type(s)	TBD
Analysis Type Detail	LC-MS
Release Date	2023-10-06
Release Version	1

Select appropriate tab below to view additional metadata details:

Factors:

Subject type: Mammal; Subject species: Rattus norvegicus (Factor headings shown in green)

mb_sample_id	local_sample_id	Group	Timepoint	Sex
SA266854	90266017008	Control	8 weeks of training or control time	Female
SA266855	90245017008	Control	8 weeks of training or control time	Female
SA266856	90248017008	Control	8 weeks of training or control time	Female
SA266857	90265017008	Control	8 weeks of training or control time	Female
SA266858	90252017008	Control	8 weeks of training or control time	Female
SA266859	90217017008	Control	8 weeks of training or control time	Male
SA266860	90229017008	Control	8 weeks of training or control time	Male
SA266861	90237017008	Control	8 weeks of training or control time	Male
SA266862	90232017008	Control	8 weeks of training or control time	Male
SA266863	90239017008	Control	8 weeks of training or control time	Male
SA266864	CS00000SB-04	QC-Blank	0 hour	-
SA266865	CS00000SB-05	QC-Blank	0 hour	-
SA266866	CS00000SB-03	QC-Blank	0 hour	-
SA266867	CS00000SB-02	QC-Blank	0 hour	-
SA266868	CS00000PB-01	QC-Blank	0 hour	-
SA266869	CS00000PB-04	QC-Blank	0 hour	-
SA266870	CS00000PB-02	QC-Blank	0 hour	-
SA266871	CS00000PB-03	QC-Blank	0 hour	-
SA266872	CS00000MP-04	QC-DriftCorrection	0 hour	-
SA266873	CS00000MP-02	QC-DriftCorrection	0 hour	-
SA266874	CS00000MP-05	QC-DriftCorrection	0 hour	-
SA266875	CS00000MP-03	QC-DriftCorrection	0 hour	-
SA266876	CS00000MP-08	QC-DriftCorrection	0 hour	-
SA266877	CS00000MP-01	QC-DriftCorrection	0 hour	-
SA266878	CS00000MP-09	QC-DriftCorrection	0 hour	-
SA266879	CS00000MP-07	QC-DriftCorrection	0 hour	-
SA266880	CS00000MP-06	QC-DriftCorrection	0 hour	-
SA266881	CS0000OP0-03	QC-Pooled	0 hour	-
SA266882	CS0000OP0-05	QC-Pooled	0 hour	-
SA266883	CS0000OP0-04	QC-Pooled	0 hour	-
SA266884	CS0000OP0-02	QC-Pooled	0 hour	-
SA266885	CS0000OP0-01	QC-Pooled	0 hour	-
SA266886	CS0000OP0-06	QC-Pooled	0 hour	-
SA266887	CS0000OP0-07	QC-Pooled	0 hour	-
SA266888	CS000QCMP-03	QC-Pooled	0 hour	-
SA266889	CS000QCMP-02	QC-Pooled	0 hour	-
SA266890	CS000QCMP-01	QC-Pooled	0 hour	-
SA266891	CS000QCMP-04	QC-Pooled	0 hour	-
SA266892	CS0UMRA01-03	QC-ReCAS	0 hour	-
SA266893	CS0UMRA01-02	QC-ReCAS	0 hour	-
SA266894	CS0UMRA01-04	QC-ReCAS	0 hour	-
SA266895	CS0UMRA01-01	QC-ReCAS	0 hour	-
SA266896	CSMR80012-03	QC-Reference	0 hour	-
SA266897	CSMR80012-02	QC-Reference	0 hour	-
SA266898	CSMR80013-04	QC-Reference	0 hour	-
SA266899	CSMR80012-04	QC-Reference	0 hour	-
SA266900	CSMR80012-01	QC-Reference	0 hour	-
SA266901	CSMR80013-03	QC-Reference	0 hour	-
SA266902	CSMR80013-02	QC-Reference	0 hour	-
SA266903	CSMR80013-01	QC-Reference	0 hour	-
SA266904	90559017008	Training	1 week of training or control time	Female
SA266905	90560017008	Training	1 week of training or control time	Female
SA266906	90567017008	Training	1 week of training or control time	Female
SA266907	90571017008	Training	1 week of training or control time	Female
SA266908	90564017008	Training	1 week of training or control time	Female
SA266909	90426017008	Training	1 week of training or control time	Male
SA266910	90423017008	Training	1 week of training or control time	Male
SA266911	90430017008	Training	1 week of training or control time	Male
SA266912	90422017008	Training	1 week of training or control time	Male
SA266913	90421017008	Training	1 week of training or control time	Male
SA266914	90576017008	Training	2 weeks of training	Female
SA266915	90587017008	Training	2 weeks of training	Female
SA266916	90578017008	Training	2 weeks of training	Female
SA266917	90585017008	Training	2 weeks of training	Female
SA266918	90581017008	Training	2 weeks of training	Female
SA266919	90441017008	Training	2 weeks of training	Male
SA266920	90449017008	Training	2 weeks of training	Male
SA266921	90444017008	Training	2 weeks of training	Male
SA266922	90439017008	Training	2 weeks of training	Male
SA266923	90450017008	Training	2 weeks of training	Male
SA266924	90420017008	Training	4 weeks of training	Female
SA266925	90406017008	Training	4 weeks of training	Female
SA266926	90410017008	Training	4 weeks of training	Female
SA266927	90412017008	Training	4 weeks of training	Female
SA266928	90416017008	Training	4 weeks of training	Female
SA266929	90280017008	Training	4 weeks of training	Male
SA266930	90292017008	Training	4 weeks of training	Male
SA266931	90283017008	Training	4 weeks of training	Male
SA266932	90281017008	Training	4 weeks of training	Male
SA266933	90289017008	Training	4 weeks of training	Male
SA266934	90251017008	Training	8 weeks of training or control time	Female
SA266935	90259017008	Training	8 weeks of training or control time	Female
SA266936	90267017008	Training	8 weeks of training or control time	Female
SA266937	90258017008	Training	8 weeks of training or control time	Female
SA266938	90254017008	Training	8 weeks of training or control time	Female
SA266939	90227017008	Training	8 weeks of training or control time	Male
SA266940	90218017008	Training	8 weeks of training or control time	Male
SA266941	90222017008	Training	8 weeks of training or control time	Male
SA266942	90223017008	Training	8 weeks of training or control time	Male
SA266943	90225017008	Training	8 weeks of training or control time	Male

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