Summary of Study ST002791

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001739. The data can be accessed directly via it's Project DOI: 10.21228/M8XD9T This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002791
Study TitleNucleotide metabolism in pancreatic cancer cells
Study SummaryThe Experiment analyzes the cancer cell metabolism in two pancreatic cancer cell lines, (i.e. Panc02 and KPC FC 1245) after the knockdown of the protein cytidine deaminase (CDA). Murine Panc02 and KPC FC1245 pancreatic cancer cell lines were genetically engineered using a doxycycline inducible CRISPR/Cas9 platform with a target specific gRNA for CDA and a control non-targeting NT gRNA. CDA knockdown cells show a significant decrease of intracellular uridine levels and the accumulation of intracellular cytidine. Consistent with the decrease in intracellular uridine, sgCda cells show reduced intracellular levels of UMP, UDP and UTP compared to sgNT cells while we see no change in adenine and cytosine nucleotides (i.e., AMP, ADP, ATP and CMP, CDP, CTP, respectively) or in UDP-hexose.
Institute
VIB-KU Leuven
Last NameMazzone
First NameMassimiliano
AddressCampus Gasthuisberg Herestraat 49, box 912 B-3000 Leuven Belgium
Emailmassimiliano.mazzone@kuleuven.vib.be
Phone+32-16-37.32.13
Submit Date2023-07-16
Raw Data AvailableYes
Raw Data File Type(s)mzML
Analysis Type DetailLC-MS
Release Date2024-01-24
Release Version1
Massimiliano Mazzone Massimiliano Mazzone
https://dx.doi.org/10.21228/M8XD9T
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Factors:

Subject type: Cultured cells; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Genotype Cell Line
SA299530KPC_CDAKD_1sgCDA KPC_FC1245
SA299531KPC_CDAKD_3sgCDA KPC_FC1245
SA299532KPC_CDAKD_4sgCDA KPC_FC1245
SA299533KPC_CDAKD_2sgCDA KPC_FC1245
SA299534Panc02_CDAKD_3sgCDA Panc02
SA299535Panc02_CDAKD_4sgCDA Panc02
SA299536Panc02_CDAKD_2sgCDA Panc02
SA299537Panc02_CDAKD_1sgCDA Panc02
SA299538KPC_NT_2sgNT KPC_FC1245
SA299539KPC_NT_1sgNT KPC_FC1245
SA299540KPC_NT_3sgNT KPC_FC1245
SA299541KPC_NT_4sgNT KPC_FC1245
SA299542Panc02_NT_4sgNT Panc02
SA299543Panc02_NT_1sgNT Panc02
SA299544Panc02_NT_2sgNT Panc02
SA299545Panc02_NT_3sgNT Panc02
Showing results 1 to 16 of 16
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