Summary of Study ST003154

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001960. The data can be accessed directly via it's Project DOI: 10.21228/M8CF0S This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST003154
Study TitleNucleotide metabolism of tumor interstitial fluid in one murine model of pancreatic cancer
Study SummaryThe experiment focus on the nucleotide metabolism of tumor interstitial fluid in one murine model of pancreatic cancer. Briefly, KPC FC1245 cells were genetically engineered using doxycycline inducible CRISPR/Cas9 system platform to target specifically cytidine deaminase (sgCda). sgCda and the control sgNT were then orthotopically injected in the head of the pancreas of 8-10 weeks old C57BL/6J female mice. When the tumor weight reached approximately 0.2-0.4g, mice were sacrificed and tumor interstitial fluid was collected as reported in the Collection section of this study. Standard curves for glutamine, cytidine, uridine, glucose, UTP and UDP were prepared and extracted along with the interstitial fluid samples. The concentrations of glutamine, cytidine, uridine, UDP and UTP were measured by LC-MS, and glucose was measured by GC-MS. A decrease in uridine content, and accordingly in UDP and UTP, and a concomitant accumulation of cytidine was observed in sgCda tumors. On the other hand, no differences were observed in glucose and glutamine abundance.
Institute
VIB-KU Leuven Center for Cancer Biology
Last NameMazzone
First NameMassimiliano
AddressHerestraat 49, box 912, Leuven, Flemish Brabant, 3000, Belgium
Emailmassimiliano.mazzone@kuleuven.be
Phone+32-16-37.32.13
Submit Date2024-03-12
Raw Data AvailableYes
Raw Data File Type(s)mzML
Analysis Type DetailGC/LC-MS
Release Date2024-04-05
Release Version1
Massimiliano Mazzone Massimiliano Mazzone
https://dx.doi.org/10.21228/M8CF0S
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Factors:

Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Sample source Genotype
SA341014STD7_bCalibration Curve N/A
SA341015STD6_bCalibration Curve N/A
SA341016STD8_bCalibration Curve N/A
SA341017STD10_bCalibration Curve N/A
SA341018STD11_bCalibration Curve N/A
SA341019STD5_bCalibration Curve N/A
SA341020STD9_bCalibration Curve N/A
SA341021STD4_bCalibration Curve N/A
SA341022STD12_aCalibration Curve N/A
SA341023STD11_aCalibration Curve N/A
SA341024STD13_aCalibration Curve N/A
SA341025STD14_aCalibration Curve N/A
SA341026STD15_aCalibration Curve N/A
SA341027STD12_bCalibration Curve N/A
SA341028STD13_bCalibration Curve N/A
SA341029STD9_cCalibration Curve N/A
SA341030STD8_cCalibration Curve N/A
SA341031STD10_cCalibration Curve N/A
SA341032STD11_cCalibration Curve N/A
SA341033STD12_cCalibration Curve N/A
SA341034STD7_cCalibration Curve N/A
SA341035STD6_cCalibration Curve N/A
SA341036STD2_cCalibration Curve N/A
SA341037STD14_bCalibration Curve N/A
SA341038STD3_cCalibration Curve N/A
SA341039STD4_cCalibration Curve N/A
SA341040STD5_cCalibration Curve N/A
SA341041STD10_aCalibration Curve N/A
SA341042STD3_bCalibration Curve N/A
SA341043STD4_aCalibration Curve N/A
SA341044STD2_aCalibration Curve N/A
SA341045STD9_aCalibration Curve N/A
SA341046STD5_aCalibration Curve N/A
SA341047STD3_aCalibration Curve N/A
SA341048STD7_aCalibration Curve N/A
SA341049STD8_aCalibration Curve N/A
SA341050STD6_aCalibration Curve N/A
SA341051TIF12Pancreatic tumor interstitial fluid sgCda
SA341052TIF7Pancreatic tumor interstitial fluid sgCda
SA341053TIF11_GCPancreatic tumor interstitial fluid sgCda
SA341054TIF6_GCPancreatic tumor interstitial fluid sgCda
SA341055TIF6Pancreatic tumor interstitial fluid sgCda
SA341056TIF7_GCPancreatic tumor interstitial fluid sgCda
SA341057TIF5_GCPancreatic tumor interstitial fluid sgCda
SA341058TIF19Pancreatic tumor interstitial fluid sgCda
SA341059TIF20Pancreatic tumor interstitial fluid sgCda
SA341060TIF21Pancreatic tumor interstitial fluid sgCda
SA341061TIF13_GCPancreatic tumor interstitial fluid sgCda
SA341062TIF11Pancreatic tumor interstitial fluid sgCda
SA341063TIF18Pancreatic tumor interstitial fluid sgCda
SA341064TIF13Pancreatic tumor interstitial fluid sgCda
SA341065TIF12_GCPancreatic tumor interstitial fluid sgCda
SA341066TIF5Pancreatic tumor interstitial fluid sgCda
SA341067TIF4Pancreatic tumor interstitial fluid sgNT
SA341068TIF2Pancreatic tumor interstitial fluid sgNT
SA341069TIF3Pancreatic tumor interstitial fluid sgNT
SA341070TIF8Pancreatic tumor interstitial fluid sgNT
SA341071TIF9Pancreatic tumor interstitial fluid sgNT
SA341072TIF10Pancreatic tumor interstitial fluid sgNT
SA341073TIF4_GCPancreatic tumor interstitial fluid sgNT
SA341074TIF15Pancreatic tumor interstitial fluid sgNT
SA341075TIF16Pancreatic tumor interstitial fluid sgNT
SA341076TIF17Pancreatic tumor interstitial fluid sgNT
SA341077TIF1Pancreatic tumor interstitial fluid sgNT
SA341078TIF14Pancreatic tumor interstitial fluid sgNT
SA341079TIF1_GCPancreatic tumor interstitial fluid sgNT
SA341080TIF9_GCPancreatic tumor interstitial fluid sgNT
SA341081TIF8_GCPancreatic tumor interstitial fluid sgNT
SA341082TIF3_GCPancreatic tumor interstitial fluid sgNT
SA341083TIF2_GCPancreatic tumor interstitial fluid sgNT
SA341084TIF10_GCPancreatic tumor interstitial fluid sgNT
Showing results 1 to 71 of 71
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