Summary of Study ST000048

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR000046. The data can be accessed directly via it's Project DOI: 10.21228/M84S36 This work is supported by NIH grant, U2C- DK119886.


Perform statistical analysis  |  Show all samples  |  Show named metabolites  |  Download named metabolite data  
Download mwTab file (text)   |  Download mwTab file(JSON)   |  Download data files (Contains raw data)
Study IDST000048
Study TitleMetabolomic & lipidomic profiles in response to exogenous insulin & GLP-1 infusions during prolonged fasting (GCMS)
Study TypeTimecourse
Study SummaryThis application requests funding to access state-of-the-art metabolomics and lipidomic platforms at the NIH West Coast Metabolomics Center to analyze plasma samples from recent insulin and glucagon-like peptide-1 (GLP-1) infusion experiments performed in prolong-fasted elephant seals. This suite of studies was designed to better assess the mechanisms contributing to the onset of an insulin resistantlike condition induced by prolonged food deprivation/starvation in mammals. Because elephant seals have evolved robust physiological mechanisms that have allowed them to naturally tolerate such protracted bouts of fasting, they provide an ideal model to address our central hypothesis that increased lipid utilization late in the fast contributes to insulin resistance in elephant seals. Insulin resistance is a common consequence of fasting in mammals and, while the mechanisms by which it manifests are still unclear, a metabolic shift favoring increased mobilization and utilization of lipids during prolonged food deprivation may be a principal causative factor. Insulin resistance has a negative connotation due to its association with obesity and diabetes among humans, but it has been suggested to be an adaptive response to food deprivation.
University of California, Davis
LaboratoryWCMC Metabolomics Core
Last NameFiehn
First NameOliver
Address451 Health Sci Drive, Davis, CA 95616
Submit Date2014-03-25
Num Groups5
Total Subjects117
Study Comments5 general classes are designed in the experiment:
#1 - A=No GLP1/ Late GTT
#2 - B=GLP1 (Low Dose)
#3 - C=GLP1 (High Dose)
#4 - IL- Early Fasting Insulin Infusion
#5 - IE- Late Fasting Insulin Infusion
Each of the 5 classes has 6 timepoints (5x6):
T1 - 0 min
T2 - 10 min
T3 - 30 min
T4 - 60 min
T5 - 120 min
Each timepoint had 5 animals (5 animals x 5 classes x 6 timepoints = 150 total
Because certain animals and timepoints had to be excluded 108 measurments remain.
The experiment also contains 9 technical replicates of pooled samples (pool)
The total sample number is 108 (biological) + 9 pooled samples = 117
Raw Data AvailableYes
Raw Data File Type(s)cdf
Uploaded File Size1.8 G
Analysis Type DetailGC-MS
Release Date2014-04-24
Release Version1
Oliver Fiehn Oliver Fiehn application/zip

Select appropriate tab below to view additional metadata details:

Combined analysis:

Analysis ID AN000084
Analysis type MS
Chromatography type GC
Chromatography system Agilent 6890N
Column Restek corporation Rtx-5Sil MS
MS Type EI
MS instrument type GC-TOF
MS instrument name Leco Pegasus III GC TOF
Units Peak Area


MS ID:MS000065
Analysis ID:AN000084
Instrument Name:Leco Pegasus III GC TOF
Instrument Type:GC-TOF
MS Type:EI
Ion Source Temperature:250°C
Ionization Energy:-70eV
Mass Accuracy:Nominal
Source Temperature:250°C
Scanning Range:80-500 Da
Acquisition Parameters File:SOP_GCTOF11082012.pdf
Processing Parameters File:Data_Dictionary_Fiehn_Laboratory_GCTOF_MS_primary_metabolism_09-27-2013_general.pdf