Summary of Study ST000885

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR000614. The data can be accessed directly via it's Project DOI: 10.21228/M8KD9Q This work is supported by NIH grant, U2C- DK119886.


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Study IDST000885
Study TitleGut Microbiota Modulate Brain Insulin Sensitivity and Neurobehavior
Study TypeMetabolite profiling of cecal contents and brains of mice under diet-induced obesity (DIO) with and without antibiotic treatments.
Study SummaryC57BL/6J mice were purchased from Jackson Laboratory and maintained on either a normal chow containing 22% of calories from fat, 23% from protein, and 55% from carbohydrates (Mouse diet 9F 5020; PharmaServ) or a high-fat diet (Open Source Diet, D12492; Research Diets) containing 60% of calories from fat, 20% from protein, and 20% from carbohydrates for 6 weeks. During the last 2 weeks, some of the HFD mice were treated with vancomycin or metronidazole (1 g/L in the drinking water). All mice were housed at 22°C on a 12 h light/dark cycle. All animal studies were approved by the IACUC of Joslin Diabetes Center (# 97-05) and Harvard Medical School (# 05131) and were in accordance with NIH guidelines. The metabolite profiling was conducted on plasma, hypothalamus and nucleus accumbens.
Broad Institute of MIT and Harvard
Last NameAvila-Pacheco
First NameJulian
Address415 Main Street
Submit Date2017-10-30
Num Groups12
Total Subjects24
Raw Data AvailableYes
Raw Data File Type(s)d
Analysis Type DetailLC-MS
Release Date2017-10-30
Release Version1
Julian Avila-Pacheco Julian Avila-Pacheco application/zip

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Combined analysis:

Analysis ID AN001442
Analysis type MS
Chromatography type HILIC
Chromatography system Agilent 1290 infinity II
Column Waters Atlantis HILIC (150 x 2mm)
MS instrument type Triple quadrupole
MS instrument name Agilent 6495 QQQ
Units Peak area


MS ID:MS001332
Analysis ID:AN001442
Instrument Name:Agilent 6495 QQQ
Instrument Type:Triple quadrupole