Summary of Study ST001261
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000846. The data can be accessed directly via it's Project DOI: 10.21228/M8B97R This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST001261 |
| Study Title | Metabolic changes of culture supernatants of Fusobacterium nucleatum co-cultured with other oral microbes (part-II) |
| Study Summary | We used membrane-separated co-culture systems to globally assess extracellular metabolomic changes of Fusobacterium nucleatum co-cultured with Streptococcus gordonii and/or Veillonella parvula. |
| Institute | Osaka University |
| Department | Graduate School of Dentistry, Department of Preventive Dentistry |
| Last Name | Kuboniwa |
| First Name | Masae |
| Address | Yamadaoka 1-8 |
| kuboniwa@dent.osaka-u.ac.jp | |
| Phone | 81668792922 |
| Submit Date | 2019-09-26 |
| Raw Data Available | Yes |
| Analysis Type Detail | LC-MS |
| Release Date | 2022-07-11 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN002092 | AN002093 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | CE | CE |
| Chromatography system | Agilent 6210 | Agilent 6210 |
| Column | None | None |
| MS Type | ESI | ESI |
| MS instrument type | CE-TOF | CE-TOF |
| MS instrument name | Agilent 6210 TOF | Agilent 6210 TOF |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | AU | AU |
MS:
| MS ID: | MS001943 |
| Analysis ID: | AN002092 |
| Instrument Name: | Agilent 6210 TOF |
| Instrument Type: | CE-TOF |
| MS Type: | ESI |
| MS Comments: | The conditions for measurement of cationic metabolites were as follows. Run buffer: Cation Buffer Solution (H3301-1001; Human Metabolome Technologies (HMT)), CE voltage: +27kV, MS ionization: ESI positive, MS capillary voltage: 4,000V, MS scan range: m/z 50-1,000, and sheath liquid: HMT Sheath Liquid (H3301-1020). Identification of metabolites and evaluation of the relative amounts were conducted using Master Hands (version 2.16.0.15 and 2.17.1.11; Keio University, Tokyo, Japan) with the HMT metabolite database. The relative amount of each metabolite was calculated with reference to the internal standard material (HMT). |
| Ion Mode: | POSITIVE |
| MS ID: | MS001944 |
| Analysis ID: | AN002093 |
| Instrument Name: | Agilent 6210 TOF |
| Instrument Type: | CE-TOF |
| MS Type: | ESI |
| MS Comments: | The conditions for measurement of anionic metabolites were as follows. Run buffer: Anion Buffer Solution (I3302-1023), CE voltage: +30kV, MS ionization: ESI negative, MS capillary voltage: 3,500V, MS scan range: m/z 50-1,000, and sheath liquid: HMT Sheath Liquid (H3301-1020). Identification of metabolites and evaluation of the relative amounts were conducted using Master Hands (version 2.16.0.15 and 2.17.1.11; Keio University, Tokyo, Japan) with the HMT metabolite database. The relative amount of each metabolite was calculated with reference to the internal standard material (HMT). |
| Ion Mode: | NEGATIVE |
