Summary of Study ST001670
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001073. The data can be accessed directly via it's Project DOI: 10.21228/M80Q4N This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST001670 |
Study Title | Metabolomics characterization of zebrafish larvae |
Study Type | MS analysis |
Study Summary | Four different treatment groups were used for metabolite characterization: 5 dpf larvae with/without beta-cell ablation and with/without folinic acid treatment. |
Institute | North Carolina State University |
Department | Molecular and Structural Biochemistry |
Last Name | Duan |
First Name | Likun |
Address | 120 W Broughton Dr. |
lduan2@ncsu.edu | |
Phone | 9195939853 |
Submit Date | 2021-01-15 |
Raw Data Available | Yes |
Raw Data File Type(s) | raw(Thermo) |
Analysis Type Detail | LC-MS |
Release Date | 2021-03-15 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN002725 | AN002726 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | HILIC | Reversed phase |
Chromatography system | Vanquish UHPLC (Thermo Fisher Scientific). | Thermo Vanquish UHPLC |
Column | Waters Xbridge amide HILIC (100 x 2.1mm,3.5um) | Luna C18 LC (150 x 4.6 mm,3um) |
MS Type | ESI | ESI |
MS instrument type | Ion trap | Ion trap |
MS instrument name | Thermo Orbitrap Exploris 480 | Thermo Orbitrap Exploris 480 |
Ion Mode | UNSPECIFIED | UNSPECIFIED |
Units | peak area | peak area |
MS:
MS ID: | MS002522 |
Analysis ID: | AN002725 |
Instrument Name: | Thermo Orbitrap Exploris 480 |
Instrument Type: | Ion trap |
MS Type: | ESI |
MS Comments: | Xcalibur 4.4 from Thermo Scientific for data acquisition and we use Sieve 2.2 for chromatographic alignment and peak integration.To analyze polar metabolites, the mass spectrometer was equipped with a HESI probe and operated in the positive/negative switching mode. The relevant parameters were as listed: vaporizer temperature, 350 °C; ion transfer tube temperature, 300 °C; sheath gas, 35; auxiliary gas, 7; sweep gas, 1; spray voltage, 3.5 kV for positive mode and 2.5 kV for negative mode; RF-lens (%), 30. The resolution was set at 60,000 (at m/z 200). Automatic maximum injection time (max IT) and automated gain control (AGC) were used. |
Ion Mode: | UNSPECIFIED |
MS ID: | MS002523 |
Analysis ID: | AN002726 |
Instrument Name: | Thermo Orbitrap Exploris 480 |
Instrument Type: | Ion trap |
MS Type: | ESI |
MS Comments: | Xcalibur 4.4 from Thermo Scientific for data acquisition and we use Sieve 2.2 for chromatographic alignment and peak integration.To measure folate derivatives, Orbitrap Exploris 480 was operated in SIM mode (selected ion monitoring) targeting the following ions: folic acid (442.1470), 5-methyltetrahydrofolate (460.1939), and folinic acid (474.1732). |
Ion Mode: | UNSPECIFIED |