Summary of Study ST001679

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001078. The data can be accessed directly via it's Project DOI: 10.21228/M8C111 This work is supported by NIH grant, U2C- DK119886.

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Study IDST001679
Study TitleQuantitative measurements of sphingomyelins in Th17 cells before and after the knockdown of UGCG gene (GCS pathway: sphingolipid metabolism) (part-V)
Study TypeMS: Untargeted and targeted analysis
Study SummaryPart 5/5: It includes measurements of sphingolipids (sphingomyelins) in Th17 cells before (scrambled / control) and after the knockdown of UGCG gene (GCS pathway: sphingolipid metabolism).
Institute
University of Turku
DepartmentSystems Medicine, Turku Bioscience
LaboratoryMetabolomics
Last NameSen
First NamePartho
AddressTykistökatu 6B, BioCity, 5th Floor, Turku, Southwest, 20521, Finland
Emailpartho.sen@utu.fi
Phone0469608145
Submit Date2021-01-31
Raw Data AvailableYes
Raw Data File Type(s)mzML
Analysis Type DetailLC-MS
Release Date2021-11-02
Release Version1
Partho Sen Partho Sen
https://dx.doi.org/10.21228/M8C111
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Combined analysis:

Analysis ID AN002737
Analysis type MS
Chromatography type Reversed phase
Chromatography system Waters Acquity UHPLC UNSPSC 41115709
Column Waters BEH C18 (00mm x 2.1mm,1.7um )
MS Type ESI
MS instrument type QTOF
MS instrument name Bruker impact II UHPLC-QTOF system
Ion Mode POSITIVE
Units ng/ml

MS:

MS ID:MS002534
Analysis ID:AN002737
Instrument Name:Bruker impact II UHPLC-QTOF system
Instrument Type:QTOF
MS Type:ESI
MS Comments:The SM results for UGCG-silenced Th17 cells data was acquired on a UHPLC-QTOF system from Bruker (Bruker, Billerica, MA, USA) combining an Elute UHPLC binary pump and an Impact II system QTOF system. The samples for this experiments were the same extracts that the Cer data was acquired from and had SM(18:1/17:0) spiked in prior to acquisition. The data was acquired using the Hystar suite of software. MZmine 2 was used for all the untargeted data processing.
Ion Mode:POSITIVE
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