Summary of Study ST002071

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001313. The data can be accessed directly via it's Project DOI: 10.21228/M80H5Z This work is supported by NIH grant, U2C- DK119886.

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This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002071
Study TitleMetabolic profiling of mouse CD27+ and CD27- gammadelta T cells
Study SummaryCultured CD27+ and CD27- gammadelta T cells were labeled with 13C-glucose for 24 h. Cells were harvested for LC-MS analysis
Institute
University of Louisville
Last NameZhang
First NameXiang
AddressShumaker Research Bld 349, Louisville, KY 40292
Emailxiang.zhang@louisville.edu
Phone502-852-8878
Submit Date2022-01-07
Raw Data AvailableYes
Raw Data File Type(s)raw(Thermo)
Analysis Type DetailLC-MS
Release Date2022-02-14
Release Version1
Xiang Zhang Xiang Zhang
https://dx.doi.org/10.21228/M80H5Z
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Combined analysis:

Analysis ID AN003376
Analysis type MS
Chromatography type HILIC
Chromatography system Q Exactive
Column SeQuant ZIC-cHILIC
MS Type ESI
MS instrument type Orbitrap
MS instrument name Thermo Exactive Plus Orbitrap
Ion Mode NEGATIVE
Units None

MS:

MS ID:MS003143
Analysis ID:AN003376
Instrument Name:Thermo Exactive Plus Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:A pooled samples for each group was detected under both positive and negative mode using 20 eV, 40eV and 60 eV. The recorded MS/MS spectrum was used for compound identification. For metabolites quantification, FullMS data of each sample was conducted under both positive and negative mode, XCMS software was used for peak deconvolution and our in-house software, MetSign was used for assignment, alignment, and statistical analysis
Ion Mode:NEGATIVE
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