Summary of Study ST002161
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001374. The data can be accessed directly via it's Project DOI: 10.21228/M84127 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002161 |
| Study Title | Glycerate Production from Intestinal Fructose Metabolism Elevated by Dietary Fat Induces Glucose Intolerance Through β-cell Damage |
| Study Summary | Dietary fructose, especially in the context of a high-fat western diet, has been linked to type 2 diabetes. Although the effect of fructose on liver metabolism has been extensively studied, a significant portion of the fructose is first metabolized in the small intestine. Here we report that dietary fat enhances intestinal fructose metabolism, which releases glycerate into the blood. High systemic glycerate levels reduce pancreatic islet sizes and β-cell content, thus inducing glucose intolerance. Our findings provide an additional link between dietary fructose and diabetes that is modulated by dietary fat. |
| Institute | Duke University |
| Last Name | Wong |
| First Name | Chi Wut |
| Address | CEIMAS, 101 Science Dr. Room 2141, Durham, NC, 27709, USA |
| chiwut.wong@duke.edu | |
| Phone | 9495290320 |
| Submit Date | 2022-05-11 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzXML |
| Analysis Type Detail | LC-MS |
| Release Date | 2022-07-06 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN003540 | AN003541 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | HILIC | IR-MALDI |
| Chromatography system | Thermo Vanquish | Other |
| Column | Waters XBridge BEH Amide (150mm x 2.1mm,2.5um) | Other |
| MS Type | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap |
| MS instrument name | Thermo Q Exactive Plus Orbitrap | Thermo Exploris 240 Orbitrap |
| Ion Mode | NEGATIVE | NEGATIVE |
| Units | Total Abundance | Peak Area |
MS:
| MS ID: | MS003298 |
| Analysis ID: | AN003540 |
| Instrument Name: | Thermo Q Exactive Plus Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | Fullscan mass spectrometry. The full scan mass spectrometry analysis was performed on a Thermo Q Exactive PLUS with a HESI source which was set to a spray voltage of -2.7kV under negative mode and 3.5kV under positive mode. The sheath, auxiliary, and sweep gas flow rates of 40, 10, and 2 (arbitrary unit), respectively. The capillary temperature was set to 300°C, and the aux gas heater was 360°C. The S-lens RF level was 45. The m/z range was set to 72 to 1000 m/z under both positive and negative ionization mode. The AGC target was set to 3e6, and the maximum IT was 200 ms. The mass resolution (full-width half maximum) was set to 70,000 @ m/z = 200. |
| Ion Mode: | NEGATIVE |
| Analysis Protocol File: | cwwong_methods.pdf |
| MS ID: | MS003299 |
| Analysis ID: | AN003541 |
| Instrument Name: | Thermo Exploris 240 Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | To ablate targeted tissue regions a 2970-nm wavelength laser was used with a single burst of ten pulses to produce 1 mJ of energy at a rate of 10 kHz. X and Y stage movements of 100 µm were used to achieve oversampling(Nazari and Muddiman, 2015). Ablated analytes were post-ionized in the orthogonal electrospray plume, established by applying a voltage of approximately 3 kV to the electrospray solvent (1 mM acetic acid in 50:50 water/acetonitrile.) Mass spectrometry analysis of ionized molecules was performed in negative mode with internal calibrant used to achieve high mass accuracy (<2.5 ppm) within the 85-225 m/z range. Automatic gain control (AGC) was disabled. A mass resolution power of 240,000FWHM at 200 m/z was used with a fixed injection time (15 ms) to synchronize timing of the ablation plume with ion collection in the C-trap of the mass spectrometer. |
| Ion Mode: | NEGATIVE |
| Analysis Protocol File: | cwwong_methods.pdf |
