Summary of Study ST002316
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001484. The data can be accessed directly via it's Project DOI: 10.21228/M8WT54 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002316 |
| Study Title | Differential requirements for mitochondrial electron transport chain components in the adult murine liver - Untargeted Metabolomics (qTOF) |
| Study Summary | Wild-type and knockout mice (Ndufa9 and Cox10) livers were harvested on liquid nitrogen. Samples were crushed on liquid nitrogen, lysed in 80% ACN, and immediately injected onto the QE. |
| Institute | University of Texas Southwestern Medical Center at Dallas |
| Department | Children's Research Institute |
| Laboratory | Prashant Mishra |
| Last Name | Lesner |
| First Name | Nicholas |
| Address | 6000 Harry Hines BLVD |
| nicholas.lesner@pennmedicine.upenn.edu | |
| Phone | 2146483784 |
| Submit Date | 2022-08-23 |
| Num Groups | 8 |
| Total Subjects | 60 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzXML |
| Analysis Type Detail | LC-MS |
| Release Date | 2022-11-01 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN003783 |
|---|---|
| Chromatography ID | CH002797 |
| MS ID | MS003526 |
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Agilent 1290 |
| Column | Waters Acquity UPLC HSS T3 (150 x 2.1mm,1.8um) |
| MS Type | ESI |
| MS instrument type | QTOF |
| MS instrument name | Agilent 6550 QTOF |
| Ion Mode | UNSPECIFIED |
| Units | area |
MS:
| MS ID: | MS003526 |
| Analysis ID: | AN003783 |
| Instrument Name: | Agilent 6550 QTOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| MS Comments: | The MS was operated in both positive and negative (ESI+ and ESI-) modes. Raw data files were processed using Profinder B.08.00 SP3 software (Agilent Technologies, CA) with an in-house database containing retention time and accurate mass information on 600 standards from Mass Spectrometry Metabolite Library (IROA Technologies, MA) which was created under the same analysis conditions. The in-house database matching parameters were mass tolerance 10 ppm; retention time tolerance 0.5 min. Peak integration result was manually curated in Profinder for improved consistency |
| Ion Mode: | UNSPECIFIED |
