Summary of Study ST002364
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001519. The data can be accessed directly via it's Project DOI: 10.21228/M8BQ4R This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST002364 |
Study Title | [U-13C]glucose tracing in activated WT, GOT1 or GLUD1 knockout CD8+ T cells |
Study Summary | WT, GOT1 knockout or GLUD1 knockout CD8+ T cells were activated with plate-bound anti-CD3 and soluble anti-CD28 for 24 hours. CD8+ T cells were pulsed with [U-13C]glucose. Intracellular glucose-derived glutamate, serine and α-ketoglutarate levels were quantified using MS. |
Institute | Johns Hopkins University |
Last Name | Xu |
First Name | Wei |
Address | 1650 Orleans Street, Baltimore, MD 21287, USA. |
wxu29@jhmi.edu | |
Phone | 443-220-9936 |
Submit Date | 2022-11-28 |
Raw Data Available | Yes |
Raw Data File Type(s) | d |
Analysis Type Detail | LC-MS |
Release Date | 2022-12-15 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN003859 |
---|---|
Analysis type | MS |
Chromatography type | Ion pair |
Chromatography system | Agilent 1290 Infinity |
Column | Agilent Zorbax Extend C18 (150 x 2.1mm,1.8 um) |
MS Type | ESI |
MS instrument type | QTOF |
MS instrument name | Agilent 6520 QTOF |
Ion Mode | NEGATIVE |
Units | AUC |
MS:
MS ID: | MS003600 |
Analysis ID: | AN003859 |
Instrument Name: | Agilent 6520 QTOF |
Instrument Type: | QTOF |
MS Type: | ESI |
MS Comments: | The optimized ESI Q-TOF parameters for MS experiments were: ion polarity, negative; gas temperature, 325 °C; drying gas, 10 L min-1; nebulizer pressure, 45 psig; capillary voltage, 4,000 V; fragmentor, 140 V; skimmer, 65 V; mass range, 50-1100 m/z; acquisition rate, 1.5 spectra s-1; instrument state, extended dynamic range (1700 m/z, 2 GHz). Spectra were internally mass calibrated in real time by continuous infusion of a reference mass solution using an isocratic pump connected to a dual sprayer feeding into an electrospray ionization source. Data were acquired with MassHunter Acquisition software. A metabolite database with retention times based on the ion-pairing method was developed using Agilent MassHunter PCDL manager software. The isotopologue peak extractions were achieved by Agilent MassHunter Profinder software. |
Ion Mode: | NEGATIVE |