Summary of Study ST002369

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR001523. The data can be accessed directly via it's Project DOI: 10.21228/M8TQ5G This work is supported by NIH grant, U2C- DK119886.


This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002369
Study TitleThe impact of acute Colony Stimulating Factor 1 treatment on serum and liver metabolites in fed and fasted mice LIVER
Study TypeDrug treatment
Study SummaryThe aim of the study was to investigate the impact of expanding tissue macrophage populations on systemic metabolism. Groups of male mice were treated with 4 x daily injections of Colony Stimulating Factor (1), followed by normal feeding or a 24 h fast from day 6 to day 7. Serum and liver were collected for GC-MS metabolomic analysis on a Shimadzu TQ8050.
University of Queensland
DepartmentMater Research-UQ
Last NameIrvine
First NameKatharine
AddressTranslational Research Institute, 37 Kent St
Submit Date2022-11-27
Num Groups4
Total Subjects20
Num Males20
Raw Data AvailableYes
Raw Data File Type(s)mzXML
Analysis Type DetailGC-MS
Release Date2023-08-01
Release Version1
Katharine Irvine Katharine Irvine application/zip

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Combined analysis:

Analysis ID AN003864
Analysis type MS
Chromatography type None (Direct infusion)
Chromatography system Shimadzu TQ8050NX
Column Agilent DB5-MS (30m)
MS instrument type Triple quadrupole
MS instrument name Shimadzu TQ8050NX
Units area


MS ID:MS003605
Analysis ID:AN003864
Instrument Name:Shimadzu TQ8050NX
Instrument Type:Triple quadrupole
MS Comments:The GC-MS system used comprised of an AOC6000 autosampler, a 2030 Shimadzu gas chromatograph and a TQ8050NX triple quadrupole mass spectrometer (Shimadzu, Japan). The mass spectrometer was tuned according to the manufacturer’s recommendations using tris-(perfluorobutyl)-amine (CF43). GC-MS was performed on a 30m Agilent DB-5 column with 0.25mm internal diameter column and 1µm film thickness. The injection temperature (inlet) was set at 280°C, the MS transfer line at 280°C and the ion source adjusted to 200°C. Helium was used as the carrier gas at a flow rate of 1 mL/min and argon gas was used in the collision cell to generate the MRM product ion. The analysis of TMS samples was performed under the following oven temperature program; 100°C start temperature, hold for 4 minutes, followed by a 10°C min-1 oven temperature ramp to 320°C with a following final hold for 11 minutes. Approximately 520 targets were collected using the Shimadzu Smart Metabolite Database, where each target comprised a quantifier MRM along with a qualifier MRM, which covers approximately 350 endogenous metabolites and multiple stable isotopically labelled internal standards. Resultant data was processed using Shimadzu LabSolutions Insight software, where peak integrations were visually validated and manually corrected where required.