Summary of Study ST002400
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001546. The data can be accessed directly via it's Project DOI: 10.21228/M8VM64 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002400 |
| Study Title | Alcohol dehydrogenase 1B is crucial for adipocyte homeostasis |
| Study Summary | Background. Alcohol dehydrogenase (ADH1B), encoded by the ADH1B gene, is a cytosolic enzyme mainly known for its role in ethanol catabolism in the liver. A few studies have paved the way to show an equally important role of ADH1B in adipocytes. This study aimed to better identify the cellular mechanisms and signaling pathways involving ADH1B in adipose tissue and to determine if ADH1B variants might contribute to adipose tissue dysfunction. Results. We showed that CRISPR-Cas9-mediated ADH1B knockout (KO) in human adipose stem cells (ASC) abolished adipocyte differentiation and decreased insulin response. This was accompanied by oxidative stress, altered mitochondrial functions, and cellular senescence. Lipidomic analysis revealed that ADH1B deficiency results in a major remodeling of lipid composition in ASC. An ADH1B homozygous loss-of-function variant was also identified in a patient presenting with a lipodystrophic and insulin resistant syndrome associated with major liver dysfunction, leading to early death. Discussion. This translational study underlines the crucial role of ADH1B in adipose tissue. It unveils cellular mechanisms accounting for its key role in adipogenesis, and adipocyte homeostasis. This study also identifies ADH1B as a candidate gene in monogenic forms of lipodystrophic and insulin resistant syndromes. |
| Institute | INSERM |
| Last Name | Gautheron |
| First Name | Jérémie |
| Address | 27 rue Chaligny |
| jeremie.gautheron@gmail.com | |
| Phone | +33623398373 |
| Submit Date | 2022-12-13 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | wiff |
| Analysis Type Detail | LC-MS |
| Release Date | 2022-12-28 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN003907 | AN003908 | AN003909 | AN003910 | AN003911 |
|---|---|---|---|---|---|
| Analysis type | MS | MS | MS | MS | MS |
| Chromatography type | HILIC | HILIC | HILIC | Reversed phase | Reversed phase |
| Chromatography system | Shimadzu 20AD | Shimadzu 20AD | Shimadzu 20AD | Shimadzu 20AD | Shimadzu 20AD |
| Column | Phenomenex Kinetex HILIC (150 x 3mm,2.6um) | Phenomenex Kinetex HILIC (150 x 3mm,2.6um) | Phenomenex Kinetex HILIC (150 x 3mm,2.6um) | Merck Supelco Ascentis Express C18 (150 x 2.1mm,2.7um) | Merck Supelco Ascentis Express C18 (150 x 2.1mm,2.7um) |
| MS Type | ESI | ESI | ESI | ESI | ESI |
| MS instrument type | QTRAP | QTRAP | QTRAP | QTRAP | QTRAP |
| MS instrument name | ABI Sciex 4000 QTrap | ABI Sciex 4000 QTrap | ABI Sciex 4000 QTrap | ABI Sciex 4000 QTrap | ABI Sciex 4000 QTrap |
| Ion Mode | POSITIVE | POSITIVE | POSITIVE | POSITIVE | POSITIVE |
| Units | mol% of total lipids | mol% of total lipids | mol% of total lipids | mol% of total lipids | mol% of total lipids |
MS:
| MS ID: | MS003646 |
| Analysis ID: | AN003907 |
| Instrument Name: | ABI Sciex 4000 QTrap |
| Instrument Type: | QTRAP |
| MS Type: | ESI |
| MS Comments: | MRM acquisition of broad chromatographic peaks of low abundant phospho- and sphingolipid classes: PS, PA, LPC, LPE and some ceramides this acquisition is called "long_1x" |
| Ion Mode: | POSITIVE |
| MS ID: | MS003647 |
| Analysis ID: | AN003908 |
| Instrument Name: | ABI Sciex 4000 QTrap |
| Instrument Type: | QTRAP |
| MS Type: | ESI |
| MS Comments: | MRM acquisition of narrow chromatographic peaks of low abundant phospho- and sphingolipid classes: cer, PG, PI, PE, PE-P This acquisition is referred to as "short_1x" |
| Ion Mode: | POSITIVE |
| MS ID: | MS003648 |
| Analysis ID: | AN003909 |
| Instrument Name: | ABI Sciex 4000 QTrap |
| Instrument Type: | QTRAP |
| MS Type: | ESI |
| MS Comments: | MRM acquisition of narrow chromatographic peaks of abundant phospho- and sphingolipid classes: PC and SM following 20-fold dilution This acquisition is referred to as "short_20x" |
| Ion Mode: | POSITIVE |
| MS ID: | MS003649 |
| Analysis ID: | AN003910 |
| Instrument Name: | ABI Sciex 4000 QTrap |
| Instrument Type: | QTRAP |
| MS Type: | ESI |
| MS Comments: | MRM acquisition of low abundant neutral lipids: DG This acquisition is referred to as "C18_1x" |
| Ion Mode: | POSITIVE |
| MS ID: | MS003650 |
| Analysis ID: | AN003911 |
| Instrument Name: | ABI Sciex 4000 QTrap |
| Instrument Type: | QTRAP |
| MS Type: | ESI |
| MS Comments: | MRM acquisition of abundant neutral lipids: CE and TG after 10 fold dilution This acquisition is referred to as "C18_10x" |
| Ion Mode: | POSITIVE |
