Summary of Study ST002730
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001694. The data can be accessed directly via it's Project DOI: 10.21228/M8R136 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002730 |
| Study Title | Multi-Omics profiling of Candida albicans from agar plate and suspension media |
| Study Type | LC/MS/MS |
| Study Summary | Candida albicans is an opportunistic pathogen that is a significant challenge to healthcare facilities worldwide, commonly found in the human gastrointestinal, respiratory, and genitourinary systems. Morphological transition allows yeast cells to diffuse through bloodstream to colonize internal organs, whilst filamentous forms is related to penetration of host mucosa and epidermal surfaces. With the help of novel analytical techniques and instruments developed in the past years, which enabled accurate, simultaneous detection and quantification of proteins and metabolites. We investigated and compared the proteome and metabolome of C. albicans grown on agar plate verses suspension culture to gain insight into the different environmental adaptation and response to stress. Multi-omics (proteomics & metabolomics) analyses were performed using a high-resolution timsTOF mass spectrometer. From the findings reported in this experiment it is worth highlighting that ease of nutritional access in suspension media favours core growth metabolism and increased translation, while impeded access in solid media favours more diverse metabolic pathways. Core growth and replication machinery are enhanced in suspension media, with several terms related to protein translation and core metabolism increased in this media. In contrast, pathogenic cell wall proteins and proteins related to cell surface were increased in cells grown on solid media. |
| Institute | University of Sharjah |
| Department | Research institute of medical and health science |
| Laboratory | Biomarker Discovery Group |
| Last Name | Facility |
| First Name | Core |
| Address | M32, SIMR, College of Pharmacy, Health Sciences, University of Sharjah, Sharjah, UAE, Sharjah, 000, United Arab Emirates |
| tims-tof@sharjah.ac.ae | |
| Phone | +971 6 5057656 |
| Submit Date | 2023-06-08 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | d |
| Analysis Type Detail | LC-MS |
| Release Date | 2023-06-25 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN004427 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Bruker timsTOF |
| Column | Hamilton Intensity Solo 2 C18 |
| MS Type | ESI |
| MS instrument type | QTOF |
| MS instrument name | Bruker timsTOF |
| Ion Mode | POSITIVE |
| Units | AU |
MS:
| MS ID: | MS004174 |
| Analysis ID: | AN004427 |
| Instrument Name: | Bruker timsTOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| MS Comments: | The ESI source conditions for every injection were as follows: the drying gas flow rate was 10.0 L/min at a temp of 220 ◦C; the capillary voltage was set at 4500 V; the End Plate offset was set at 500 V; the nebulizer pressure of 2.2 bar. The acquisition involved two segments; auto MS scan, which ranged from 0 to 0.3 min for the calibrant sodium formate, and auto MS/MS scan with CID acquisition, which included fragmentation and ranged from 0.3 to 30 min. The acquisition in both segments was performed using the positive mode at 12 Hz. The automatic in-run mass scan range was from 20 to 1300 m/z, the width of the precursor ion was ±0.5, the number of precursors was 3, the cycle time was 0.5 sec., and the threshold was 400 cts. Active exclusion was excluded after 3 spectra and released after 0.2 min. For MS2 acquisition the data dependent acquisition (DDA) was used, and the collision energy stepping fluctuated between 100 and 250% set at 20 eV. m/z measurements were externally calibrated using 10 mM of sodium formate before sample analysis. In addition, sodium formate solution was injected at the beginning of each sample run and used for internal calibration during data processing. |
| Ion Mode: | POSITIVE |
