Summary of Study ST002732
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001696. The data can be accessed directly via it's Project DOI: 10.21228/M8GM73 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002732 |
| Study Title | Impaired metabolism predicts coronary artery calcification in women with systemic lupus erythematosus |
| Study Summary | Background. Patients with systemic lupus erythematosus (SLE) exhibit a high risk for cardiovascular diseases which is not fully explained by the classical Framingham risk factors. SLE is characterized by major metabolic alterations which could contribute to the elevated prevalence of CVD. In order to address this hypothesis, a comprehensive analysis of the circulating metabolome and lipidome was conducted in a large cohort of 211 women with SLE who underwent a multi-detector computed tomography (CT) scan for quantification of coronary artery calcium (CAC), a robust predictor of coronary heart disease (CHD). Results. Beyond traditional risk factors, including age and hypertension, disease activity and duration were independent risk factor for developing CAC in women with SLE. The presence of coronary calcium was associated with major alterations of circulating lipidome dominated by a high abundance of circulating ceramides with very long chain fatty acids. Alteration in multiple metabolic pathways, including purine metabolism, arginine and proline metabolism, and microbiota-derived metabolites, were also associated with CAC in women with SLE. Backward stepwise logistic regression models of lipidomic and metabolomic variables were used to develop prognostic scores. Strikingly, combining metabolic and lipidomic variables to clinical and biological parameters markedly improved the prediction (Area under the curve: 0.887, P<0.001) of the presence of coronary calcium in women with SLE. Conclusion. The present study uncovers the contribution of disturbed metabolism in the presence of coronary artery calcium and the prediction of CHD in SLE. The identification of these novel lipid and metabolite biomarkers may help to stratify patients for reducing CVD morbidity and mortality in SLE. |
| Institute | INSERM |
| Last Name | Le Goff |
| First Name | Wilfried |
| Address | 91, bd de l'Hopital |
| wilfried.le_goff@sorbonne-universite.fr | |
| Phone | +33140779638 |
| Submit Date | 2023-06-07 |
| Num Groups | 3 |
| Total Subjects | 228 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | wiff |
| Analysis Type Detail | LC-MS |
| Release Date | 2023-06-25 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN004429 | AN004430 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | HILIC | HILIC |
| Chromatography system | Shimadzu 20AD | Shimadzu 20AD |
| Column | Phenomenex Kinetex HILIC (150 x 3mm,2.6um) | Phenomenex Kinetex HILIC (150 x 3mm,2.6um) |
| MS Type | ESI | ESI |
| MS instrument type | QTRAP | QTRAP |
| MS instrument name | ABI Sciex 4000 QTrap | ABI Sciex 4000 QTrap |
| Ion Mode | POSITIVE | POSITIVE |
| Units | relative difference | relative difference |
MS:
| MS ID: | MS004176 |
| Analysis ID: | AN004429 |
| Instrument Name: | ABI Sciex 4000 QTrap |
| Instrument Type: | QTRAP |
| MS Type: | ESI |
| MS Comments: | MRM acquisition of low abundant phospho- and sphingolipid classes: PS, PA, LPC, LPE, cer, PG, PI, PE, PE-P this acquisition is called "short" |
| Ion Mode: | POSITIVE |
| MS ID: | MS004177 |
| Analysis ID: | AN004430 |
| Instrument Name: | ABI Sciex 4000 QTrap |
| Instrument Type: | QTRAP |
| MS Type: | ESI |
| MS Comments: | MRM acquisition of abundant lipids following 100fold dilution for PC and SM analysis. This acquisition is called "short10x" |
| Ion Mode: | POSITIVE |
