Summary of Study ST002740
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001705. The data can be accessed directly via it's Project DOI: 10.21228/M89T4G This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002740 |
| Study Title | Non-targeted metabolomics screen comparing 13C2-acetate labeling of metabolites in CD8+ T cells and NK cells from mouse spleens. |
| Study Summary | Non-targeted metabolomics screen comparing 13C2-acetate labeling of metabolites in CD8+ T cells and NK cells from mouse spleens (wild type vs ACSS2 knockout C57Bl/6 mice). Metabolites were analyzed using a high-resolution, high-performance LC-MS analysis. |
| Institute | The Wistar Institute |
| Last Name | Schug |
| First Name | Zachary |
| Address | 3601 Spruce St, Philadelphia PA 19104 |
| zschug@wistar.org | |
| Phone | 215-898-3705 |
| Submit Date | 2023-06-20 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | raw(Thermo) |
| Analysis Type Detail | LC-MS |
| Release Date | 2023-07-11 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN004443 |
|---|---|
| Analysis type | MS |
| Chromatography type | HILIC |
| Chromatography system | Thermo Vanquish |
| Column | SeQuant ZIC-pHILIC (150 x 2.1mm, 5um) |
| MS Type | ESI |
| MS instrument type | Orbitrap |
| MS instrument name | Thermo Q Exactive HF-X Orbitrap |
| Ion Mode | UNSPECIFIED |
| Units | Peak Area |
MS:
| MS ID: | MS004190 |
| Analysis ID: | AN004443 |
| Instrument Name: | Thermo Q Exactive HF-X Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | The following parameters were used for the MS analysis: sheath gas flow rate, 30; auxiliary gas flow rate, 5; sweep gas flow rate, 0; auxiliary gas heater temperature, 200 °C; spray voltage, 3.6 kV for positive and negative modes; capillary temperature, 325 °C; and funnel RF level, 65. Samples were analyzed by full MS with polarity switching. Full MS scans were acquired at 60,000 resolution with 2 microscans and a scan range of 72-1080 m/z, automatic gain control (AGC) target of 5e6, and maximum injection time (IT) of 200 ms. |
| Ion Mode: | UNSPECIFIED |
