Summary of Study ST001053

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR000706. The data can be accessed directly via it's Project DOI: 10.21228/M8DT2N This work is supported by NIH grant, U2C- DK119886.


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Study IDST001053
Study Title1H-NMR Analysis of Skin and Blubber of Nose Dolphin Metabolome Reveal the Functional Metabolomic Dichotomy of The Organs
Study TypeDolphin Skin and Blubber
Study SummaryThe common bottlenose dolphin (Tursiops truncatus) is carnivorous cetacean thriving in marine environment are one of the most common apex predators found in coastal and estuarine ecosystems. Although recent studies have focused on capturing the circulating metabolomes of these organisms, with respect to pollutants and exposures of the marine environment, the skin and blubber are important protective organs that have not been probed. Using 1HNMR based untargeted metabolomics we quantified 51 metabolites belonging to 74 different metabolic pathways in the skin and blubber of bottle nose dolphins (n=5) samples collected in 2017 from the coast of Mexico. Results indicate that the skin and blubber metabolism are quantitatively different. These metabolite abundances could help discriminate the tissue-types using supervised and unsupervised PCA and PLSDA analysis. Heat maps and random forest analysis point to unique metabolites that are important classifiers of the tissue-type. The altered metabolic patterns, mainly linking fatty acid metabolism and ketogenic amino acids, seem to constitute a characteristic of blubber, while the skin showed diverse metabolites involved in gluceoneogenic pathways. 1H NMR spectra allowed the identification of metabolites associated with these organ types, such as pyruvic acid, arginine, ornithine, 2-hydroxybutyric acid, 3-hydroxyisobutyric acid, and acetic acid, as discriminatory and classifying metabolites. These results would lead to further understanding of dolphin skin and blubber metabolism for better efforts in their conservation as well as a measure of marine pollution and ecotoxicology.
Wake Forest Baptist Medical Center
DepartmentDepartment of Internal Medicine
Last NameMisra
First NameBiswapriya
AddressMedical Center Boulevard NRC Building, G#43, Medical Center Boulevard, Winston Salem, NC, 27157, USA
Submit Date2018-09-12
Num Groups2
Total Subjects4
Num Males3
Num Females1
Study CommentsNA
PublicationsDOI: 1H-NMR Metabolomic Analysis of Skin and Blubber of Bottlenose Dolphins Reveal a Functional Metabolic Dichotomy, Comparative Biochemistry and Physiology - Part D: Genomics and Proteomics Author List: Biswapriya Misra, Ixchel Ruiz-Hernández, Gloria Ivonne Hernández-Bolio, Emanuel Hernandez Nuñez, Raúl Díaz-Gamboa, Reyna Cristina Collí Dulá
Raw Data AvailableYes
Raw Data File Type(s)fid
Analysis Type DetailNMR
Release Date2019-12-17
Release Version1
Biswapriya Misra Biswapriya Misra application/zip

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NMR ID:NM000128
Analysis ID:AN001722
Instrument Name:Varian 600 MHz AR Premium Compact
Instrument Type:FT-NMR
NMR Experiment Type:1D 1H
Spectrometer Frequency:600 MHz
NMR Probe:OneProbe
NMR Solvent:D2O
NMR Tube Size:5 mm
Shimming Method:Gradient shimming
Pulse Sequence:PRESAT
Water Suppression:Yes, satmode
Pulse Width:5.90 (90°)
Power Level:63
Receiver Gain:20
Presaturation Power Level:20
Chemical Shift Ref Cpd:0 ppm (TSP)
Number Of Scans:128
Acquisition Time:3
Relaxation Delay:2
Spectral Width:9615.4
Num Data Points Acquired:64000
Real Data Points:57692
Chemical Shift Ref Std:TSP