Summary of Study ST001617
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001039. The data can be accessed directly via it's Project DOI: 10.21228/M8D400 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST001617 |
| Study Title | Unique metabolomic profile of skeletal muscle in chronic limb threatening ischemia (HR-MAS study) |
| Study Summary | This project is focused on a cross-sectional analysis of non-PAD controls and CLTI patients undergoing either a vascular intervention or undergoing limb amputation was performed and involved a detailed assessment of the limb muscle metabolome using HR-MAS and solution state NMR spectroscopy. It was hypothesized that patients undergoing limb amputation would present with altered muscle metaolite features compared with non-PAD controls. |
| Institute | University of Florida |
| Department | Applied Physiology and Kinesiology |
| Laboratory | Rm 42 and Rm 43 |
| Last Name | Ryan |
| First Name | Terence |
| Address | 1864 Stadium Rd, FLG 114, Gainesville, FL, 32611, USA |
| ryant@ufl.edu | |
| Phone | 352-294-1700 |
| Submit Date | 2020-11-30 |
| Num Groups | 3 |
| Total Subjects | 30 |
| Num Males | 25 |
| Num Females | 5 |
| Study Comments | PAD metabolomic study via NMR. A detailed assessment of the limb muscle metabolome using semi-solid and solution state NMR spectroscopy. |
| Publications | Journal of Clinical Medicine |
| Raw Data Available | Yes |
| Raw Data File Type(s) | fid |
| Analysis Type Detail | NMR |
| Release Date | 2022-07-07 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
NMR:
| NMR ID: | NM000189 |
| Analysis ID: | AN002652 |
| Instrument Name: | Bruker Avance 800 MHz with HR-MAS console |
| Instrument Type: | FT-NMR |
| NMR Experiment Type: | 1D-1H |
| Field Frequency Lock: | Deuterium |
| Standard Concentration: | None |
| Spectrometer Frequency: | 800 MHz |
| NMR Probe: | HR-MAS probe |
| NMR Solvent: | For HR-MAS experiments, deuterium oxide was used. |
| NMR Tube Size: | 3.2 mm inside diameter plastic insert was used for HR-MAS experiments. |
| Shimming Method: | Topshim |
| Pulse Sequence: | noesypr1d |
| Water Suppression: | presat |
| Pulse Width: | 90-degree |
| Receiver Gain: | 28.5 |
| Offset Frequency: | 4.96 Hz |
| Chemical Shift Ref Cpd: | None |
| Temperature: | 4 oC |
| Number Of Scans: | 256 |
| Dummy Scans: | 8 |
| Acquisition Time: | 2.0447 s |
| Relaxation Delay: | 2 s |
| Spectral Width: | 8012.8 Hz |
| Num Data Points Acquired: | 16384 points |
| Real Data Points: | 65536 |
| Line Broadening: | 0.5 Hz |
| Zero Filling: | 65,536 points |
| Apodization: | Exponential |
| Baseline Correction Method: | Spline |
| Chemical Shift Ref Std: | Alanine doublet at 1.46 ppm |
