Summary of Study ST001630

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR001042. The data can be accessed directly via it's Project DOI: 10.21228/M80X2Z This work is supported by NIH grant, U2C- DK119886.


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Study IDST001630
Study TitleTissue-specific 1H-NMR metabolomic profiling in mice with adenine-induced chronic kidney disease - aqueous phase Quadricep (part-VII)
Study TypeMetabolomic profiling of the heart, liver, kidney, and skeletal muscles obtained from mice with and without CKD via 1H NMR
Study SummaryThis project is focused on a metabolomic analyses of the heart, liver, kidney, and skeletal muscles obtained from mice with and without CKD. To accomplish this objective, we extracted tissues from mice with CKD induced by long-term (24 week) adenine-supplemented diet as well as their control-diet fed counterparts with normal kidney function. Metabolites were extracted from tissues and 1H nuclear magnetic resonance (NMR) was performed and coupled with multivariate statistical analysis.
University of Florida
DepartmentApplied Physiology and Kinesiology
LaboratoryRm 42 and Rm 43
Last NameRyan
First NameTerence
Address1864 Stadium Rd, FLG 114, Gainesville, FL, 32611, USA
Submit Date2020-12-11
Num Groups2
Total Subjects17
Num MalesAll
Study CommentsCKD metabolomic study via NMR using mice model
Raw Data AvailableYes
Raw Data File Type(s)fid
Analysis Type DetailNMR
Release Date2021-06-30
Release Version1
Terence Ryan Terence Ryan application/zip

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NMR ID:NM000197
Analysis ID:AN002665
Instrument Name:Bruker Avance Neo 600 MHz/54mm console
Instrument Type:FT-NMR
NMR Experiment Type:1D-1H
Field Frequency Lock:Deuterium
Standard Concentration:0.5 mM DSS
Spectrometer Frequency:600.2328273 MHz
NMR Probe:1.7 mm TXI CryoProbe
NMR Solvent:Phosphate buffer (pH 7.2) + 2 mM EDTA + 0.5 mM DSS + 0.2% of sodium azide in deuterated environment
NMR Tube Size:1.7 mm O.D.
Shimming Method:Topshim
Pulse Sequence:noesypr1d
Water Suppression:presat
Pulse Width:90-degree
Receiver Gain:101
Offset Frequency:2827.31 Hz
Chemical Shift Ref Cpd:DSS (4,4-dimethyl-4-silapentane-1-sulfonic acid)
Temperature:25 o C
Number Of Scans:128 scans
Dummy Scans:8
Acquisition Time:4 s
Relaxation Delay:1 s
Spectral Width:7142.9 Hz
Num Data Points Acquired:28571
Real Data Points:65536
Line Broadening:0.22 Hz
Zero Filling:65,536 points
Baseline Correction Method:Spline
Chemical Shift Ref Std:0 ppm for DSS