Summary of Study ST000904
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000628. The data can be accessed directly via it's Project DOI: 10.21228/M8H10W This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000904 |
| Study Title | Measurement of 2-hydroxyglutarate for reproducibility project |
| Study Summary | Measurement of D-2-hydroxyglutarate and comparison of peak area with glutamate |
| Institute | Sanford Burnham Prebys Medical Discovery Institute |
| Last Name | Scott |
| First Name | David |
| Address | 10901 N. Torrey Pines Road |
| dscott@sbpdiscovery.org | |
| Phone | 858-646-3100 |
| Submit Date | 2017-11-22 |
| Raw Data File Type(s) | cdf |
| Analysis Type Detail | GC-MS |
| Release Date | 2018-02-07 |
| Release Version | 1 |
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Project:
| Project ID: | PR000628 |
| Project DOI: | doi: 10.21228/M8H10W |
| Project Title: | Measurement of 2-hydroxyglutarate for reproducibility project |
| Project Summary: | The Reproducibility Project: Cancer Biology seeks to address growing concerns about reproducibility in scientific research by conducting replications of selected experiments from a number of high-profile papers in the field of cancer biology. The papers, which were published between 2010 and 2012, were selected on the basis of citations and Altmetric scores (Errington et al., 2014). This Registered Report describes the proposed replication plan of key experiments from IDH mutation impairs histone demethylation and results in a block to cell differentiation by Lu and colleagues, published in Nature in 2012 (Lu et al., 2012). The experiments that will be replicated are those reported in Figures 1B, 2A, 2B, 2D and 4D. Lu and colleagues demonstrated that expression of mutant forms of IDH1 or IDH2 caused global increases in histone methylation and increased levels of 2 hydroxyglutarate (Figure 1B). This was correlated with a block in differentiation (Figures 2A, B and D). This effect appeared to be mediated by the histone demethylase KDM4C (Figure 4D). The Reproducibility Project: Cancer Biology is a collaboration between the Center for Open Scienceand Science Exchange, and the results of the replications will be published by eLife. |
| Institute: | Sanford Burnham Prebys Medical Discovery Institute |
| Last Name: | Scott |
| First Name: | David |
| Address: | 10901 N. Torrey Pines Road, La Jolla, California, 92037, USA |
| Email: | dscott@sbpdiscovery.org |
| Phone: | 858-646-3100 |
