Summary of Study ST001107

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR000740. The data can be accessed directly via it's Project DOI: 10.21228/M81D57 This work is supported by NIH grant, U2C- DK119886.


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Study IDST001107
Study TitleNMR Metabolomics of Newborn Heart Tissue Exposed to Excess Maternal Cortisol in Late Gestation (part -II)
Study TypeComparison
Study SummaryCardiac tissue from newborn hearts from animals exposed to excess maternal cortisol in late gestation and untreated was compared via NMR metabolomic analysis
University of Florida
DepartmentBiochemsitry & Molecular Biology
Last NameWalejko
First NameJacquelyn
AddressR3-226 Academic Research Building, Department of Biochemistry and Molecular Biology, PO Box 100245, Gainesville, FL 32610-0245
Submit Date2018-12-04
Num Groups2
Total Subjects36
Raw Data AvailableYes
Raw Data File Type(s)fid
Analysis Type DetailNMR
Release Date2019-03-06
Release Version1
Jacquelyn Walejko Jacquelyn Walejko application/zip

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Project ID:PR000740
Project DOI:doi: 10.21228/M81D57
Project Title:Chronic Maternal Cortisol Excess During Late Gestation Leads to Metabolic Alterations in the Newborn Heart
Project Summary:Our laboratory has previously shown in an ovine model of pregnancy that abnormal elevations in maternal cortisol during late gestation lead to increased fetal cardiac arrhythmias and mortality during peripartum. Furthermore, transcriptomic analysis of the fetal heart suggested alterations in TCA cycle intermediates and lipid metabolites in animals exposed to excess cortisol in utero. Therefore, we utilized a sheep model of pregnancy to determine how chronic increases in maternal cortisol alter maternal and fetal serum prior to birth and neonatal cardiac metabolites and lipids at term. Ewes were either infused with 1 mg/kg/day of cortisol starting at gestational day 115 (n=9), or untreated (n=6). Serum was collected from the mother and fetus (125 d-birth), and hearts were collected following birth. Proton nuclear magnetic resonance (1H-NMR) spectroscopy was conducted to measure metabolic profiles of newborn heart specimens as well as fetal and maternal serum specimens. Mass spectrometry was conducted to measure lipid profiles of newborn heart specimens. We observed alterations in amino acid and TCA cycle metabolism as well as lipid and glycerophospholipid metabolism in newborn hearts after excess maternal cortisol in late gestation. In addition, we observed alterations in amino acid and TCA cycle metabolites in fetal but not in maternal serum during late gestation. These results suggest that fetal exposure to excess maternal cortisol alters placental and fetal metabolism prior to birth and limits normal cardiac metabolic maturation, which may contribute to increased risk of peripartum cardiac arrhythmias observed in these animals, or later life cardiomyopathies.
Institute:University of Florida
Last Name:Keller-Wood
First Name:Maureen
Address:1345 SW Archer Rd, PO 100487, Gainesville, FL, 32610