Summary of Study ST001452
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000998. The data can be accessed directly via it's Project DOI: 10.21228/M8PQ57 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST001452 |
| Study Title | Targeted metabolomic analysis on hexosamine biosynthetic pathway in flies on time restricted feeding |
| Study Type | metabolomic identification |
| Study Summary | The integration of circadian and metabolic signals is essential for maintaining robust circadian rhythms and ensuring efficient metabolism and energy use. Using Drosophila as an animal model, we showed observed strong correlation between daily daily rhythms of protein O-linked N-acetylglucosaminylation (O-GlcNAcylation) and clock-controlled feeding-fasting cycles, suggesting that O-GlcNAcylation rhythms are primarily driven by nutrient input. Interestingly, daily O-GlcNAcylation rhythms were severely dampened when we subjected flies to time-restricted feeding (TRF) at unnatural feeding time. This suggests the presence of a clock-regulated buffering mechanism that prevents excessive O-GlcNAcylation at non-optimal times of the day-night cycle, which could disrupt circadian health. We performed targeted metabolomic analysis on hexosamine biosynthetic pathway (HBP), which produces UDP-GlcNAc (the substrate for O-GlcNAcylation), to evaluate the daily activity of HBP enzymes under TRF conditions. We found glutamine--fructose-6-phosphate amidotransferase (GFAT) mediates this buffering mechanism. |
| Institute | University of California, Davis |
| Department | Department of Entomology and Nematology |
| Laboratory | Chiu lab |
| Last Name | Chiu |
| First Name | Joanna |
| Address | One Shield Ave, Davis CA 95695 |
| jcchiu@ucdavis.edu | |
| Phone | (530) 752-1839 |
| Submit Date | 2020-08-13 |
| Num Groups | 2 |
| Total Subjects | 72 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | wiff |
| Analysis Type Detail | LC-MS |
| Release Date | 2020-09-10 |
| Release Version | 1 |
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Project:
| Project ID: | PR000998 |
| Project DOI: | doi: 10.21228/M8PQ57 |
| Project Title: | Targeted metabolomic analysis on hexosamine biosynthetic pathway in flies on time restricted feeding |
| Project Type: | Targeted analysis by HILIC |
| Project Summary: | The integration of circadian and metabolic signals is essential for maintaining robust circadian rhythms and ensuring efficient metabolism and energy use. Using Drosophila as an animal model, we showed observed strong correlation between daily daily rhythms of protein O- linked N-acetylglucosaminylation (O-GlcNAcylation) and clock-controlled feeding-fasting cycles, suggesting that O-GlcNAcylation rhythms are primarily driven by nutrient input. Interestingly, daily O-GlcNAcylation rhythms were severely dampened when we subjected flies to time- restricted feeding (TRF) at unnatural feeding time. This suggests the presence of a clock- regulated buffering mechanism that prevents excessive O-GlcNAcylation at non-optimal times of the day-night cycle, which could disrupt circadian health. We performed targeted metabolomic analysis on hexosamine biosynthetic pathway (HBP), which produces UDP-GlcNAc (the substrate for O-GlcNAcylation), to evaluate the daily activity of HBP enzymes under TRF conditions. We found glutamine--fructose-6-phosphate amidotransferase (GFAT) mediates this buffering mechanism. |
| Institute: | University of California, Davis |
| Department: | Department of Entomology and Nematology |
| Laboratory: | Chiu Lab |
| Last Name: | Chiu |
| First Name: | Joanna |
| Address: | One Shield Ave, Davis CA 95695 |
| Email: | jcchiu@ucdavis.edu |
| Phone: | 5307521839 |
| Funding Source: | National Institutes of Health grants R01 GM102225 and R01 DK124068 to JCC |
