Summary of Study ST002383

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR001533. The data can be accessed directly via it's Project DOI: 10.21228/M8J99C This work is supported by NIH grant, U2C- DK119886.


This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002383
Study TitleMetabolomics of B-cell Acute Lymphoblastic Leukemia in Response to Adipocyte Conditioned Media
Study TypeUntargeted MS
Study SummaryAdipocyte conditioned media (ACM), stromal cell conditioned media (SCM) and unconditioned media (UCM) were added to B-cell Acute Lymphoblastic Leukemia cells (REH and RCH-AcV) either with or without methotrexate (MTX). The metabolomic profiles of the cells was determined by mass spectrometry.
Emory University
LaboratoryJoshua Chandler, PhD
Last NameChandler
First NameJoshua
Address2015 Uppergate Drive NE, Atlanta, GA 30322
Submit Date2022-10-07
Num GroupsTwo sets of two sets of three groups (MTX and no-MTX, REH and RCH-AcV, ACM vs SCM vs UCM)
Total Subjects3 replicates of each group (36 total)
Publicationssubmitted to JNCI
Raw Data AvailableYes
Raw Data File Type(s)mzML
Analysis Type DetailLC-MS
Release Date2023-06-07
Release Version1
Joshua Chandler Joshua Chandler application/zip

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Project ID:PR001533
Project DOI:doi: 10.21228/M8J99C
Project Title:The 'Omics of Obesity in B-cell Acute Lymphoblastic Leukemia
Project Summary:The obesity pandemic currently affects over 70 million Americans and over 650 million individuals worldwide. In addition to increasing susceptibility to pathogenic infections (e.g., SARS-CoV-2 et al.), obesity promotes the development of many cancer subtypes and increases mortality rates in most cases. It has been demonstrated that, in the context of B-cell acute lymphoblastic leukemia (B-ALL), adipocytes promote multi-drug chemoresistance. Furthermore, it has been demonstrated that B-ALL cells exposed to the adipocyte secretome alter their metabolic states to circumvent chemotherapy-mediated cytotoxicity. To better understand how adipocytes impact the function of human B-ALL cells, we used an untargeted metabolomics mass spectroscopy approach to define adipocyte-induced changes in B-cells. These analyses revealed that the adipocyte secretome directly modulates programs in human B-ALL cells which are associated with metabolism. In all, our data increases our understanding of pathways which may promote chemoresistance in human B-ALL.
Institute:Emory University
Laboratory:Joshua Chandler, PhD
Last Name:Chandler
First Name:Joshua
Address:2015 Uppergate Drive NE, Atlanta, GA 30322
Funding Source:This study was supported by funding for the CURE Childhood Cancer Foundation (Grant No. 001006916), Swim Across America (Grant No. 00103163), The Mark Foundation for Cancer Research (Grant No. 18-031-ASP), Emory University School of Medicine Bridge Funding (Grant No. 00098174), The American Cancer Society and Emory University Winship Cancer Institute Institutional Research Grant (Grant No. IRG-21-137-07-IRG) and the TREC Training Course (Grant No. R25CA203650) all awarded to Curtis J Henry. In additional, funding for Joshua Chandler included NIH R01 NR018666, R56 HL150658, and support from CF@LANTA, a component of Emory University and Children’s Healthcare of Atlanta.
Publications:submitted to JNCI
Contributors:Delaney K. Geitgey, Miyoung Lee, Kirsten A. Cottrill, Matthew B. Kilgore, Joshua D. Chandler, Curtis J. Henry