Summary of Study ST000258

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR000210. The data can be accessed directly via it's Project DOI: 10.21228/M87W2S This work is supported by NIH grant, U2C- DK119886.


This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

Perform statistical analysis  |  Show all samples  |  Show named metabolites  |  Download named metabolite data  
Download mwTab file (text)   |  Download mwTab file(JSON)   |  Download data files (Contains raw data)
Study IDST000258
Study TitleMetabolic contribution of pSymA and pSymB megaplasmid/chromid for multipartite Sinorhizobium meliloti cultured in minimal M9 medium
Study Typemegaplasmid deletion
Study SummaryTo understand the contribution of pSymA and pSymB to the metabolism of S. meliloti, the intracellular metabolome was analyzed at five time points (exponential and stationary growth phases) across the growth curve of strains with or without pSymA and/or pSymB grown in a defined, minimal medium (M9).
McMaster University
DepartmentDepartment of Biology
Last NameFinan
First NameTurlough
AddressDepartment of Biology, McMaster University, Hamilton, Canada L8S4K1
Phone(+1)905-525-9140 ext 22932
Submit Date2015-09-17
Num Groups20
Total Subjects115
Raw Data AvailableYes
Raw Data File Type(s)mzXML
Analysis Type DetailLC-MS
Release Date2015-10-26
Release Version1
Turlough Finan Turlough Finan application/zip

Select appropriate tab below to view additional metadata details:

Sample Preparation:

Sampleprep ID:SP000285
Sampleprep Summary:-
Sampleprep Protocol Filename:Fei,_F.,_Bowdish,_D._M._E.,_&_McCarry,_B._E._(2014)._Analytical_and_Bioanalytical_Chemistry,_406,_3723–3733.
Extraction Method:cell pellet extracted with 2:2:1 MeOH/EtOH/H2O with beadbeating
Extract Storage:-80ºC freezer
Sample Resuspension:cell extracts suspended 60%v/v acetonitrile/water
Sample Spiking:L-methionine-d3, L-tryptophan-d5 as standards for recovery determination ; L-phenylalanine-d8, diphenylalanine and glycine-phenylalanine as internal standards for peak area normalization
Cell Type:Gram negative bacterium